Abstract
Thymosin-βs (Tβs) are water-soluble peptides abundantly present in the cytoplasm and extracellular compartment. The functions of Tβs appear to be pleiotrophic, including actin-remodeling, wound healing, angiogenesis, etc. In the present study, we present the evidence that Tβs have anti-apoptotic activity on developing chick motoneurons (MNs) in vivo. Using in ovo electroporation, we introduced three isoforms of Tβ (Tβ4, Tβ10, and Tβ15) and found the significantly diminished normal and limb bud removal (LBR)-induced programmed cell death. Such anti-apoptotic activity is independent of Tβ's actin remodeling activity. On the other hand, overexpression of Tβs substantially reduced early cell death initiation signal, such as phosphorylation of c-Jun. Collectively, these results suggest that Tβs may prevent apoptosis of neurons via blockade of early apoptogenic signals independent of actin remodeling action.
| Original language | English |
|---|---|
| Pages (from-to) | 872-878 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 346 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2006 Aug 4 |
| Externally published | Yes |
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Keywords
- Chick embryo
- Jun
- Motoneuron
- Programmed cell death
- Thymosin-β
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Anti-apoptotic function of thymosin-β in developing chick spinal motoneurons. / Choi, So Yoen; Kim, Dong Ki; Eun, Bokkee; Kim, Kyungjin; Sun, Woong; Kim, Hyun.
In: Biochemical and Biophysical Research Communications, Vol. 346, No. 3, 04.08.2006, p. 872-878.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anti-apoptotic function of thymosin-β in developing chick spinal motoneurons
AU - Choi, So Yoen
AU - Kim, Dong Ki
AU - Eun, Bokkee
AU - Kim, Kyungjin
AU - Sun, Woong
AU - Kim, Hyun
PY - 2006/8/4
Y1 - 2006/8/4
N2 - Thymosin-βs (Tβs) are water-soluble peptides abundantly present in the cytoplasm and extracellular compartment. The functions of Tβs appear to be pleiotrophic, including actin-remodeling, wound healing, angiogenesis, etc. In the present study, we present the evidence that Tβs have anti-apoptotic activity on developing chick motoneurons (MNs) in vivo. Using in ovo electroporation, we introduced three isoforms of Tβ (Tβ4, Tβ10, and Tβ15) and found the significantly diminished normal and limb bud removal (LBR)-induced programmed cell death. Such anti-apoptotic activity is independent of Tβ's actin remodeling activity. On the other hand, overexpression of Tβs substantially reduced early cell death initiation signal, such as phosphorylation of c-Jun. Collectively, these results suggest that Tβs may prevent apoptosis of neurons via blockade of early apoptogenic signals independent of actin remodeling action.
AB - Thymosin-βs (Tβs) are water-soluble peptides abundantly present in the cytoplasm and extracellular compartment. The functions of Tβs appear to be pleiotrophic, including actin-remodeling, wound healing, angiogenesis, etc. In the present study, we present the evidence that Tβs have anti-apoptotic activity on developing chick motoneurons (MNs) in vivo. Using in ovo electroporation, we introduced three isoforms of Tβ (Tβ4, Tβ10, and Tβ15) and found the significantly diminished normal and limb bud removal (LBR)-induced programmed cell death. Such anti-apoptotic activity is independent of Tβ's actin remodeling activity. On the other hand, overexpression of Tβs substantially reduced early cell death initiation signal, such as phosphorylation of c-Jun. Collectively, these results suggest that Tβs may prevent apoptosis of neurons via blockade of early apoptogenic signals independent of actin remodeling action.
KW - Chick embryo
KW - Jun
KW - Motoneuron
KW - Programmed cell death
KW - Thymosin-β
UR - http://www.scopus.com/inward/record.url?scp=33745209636&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745209636&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2006.05.207
DO - 10.1016/j.bbrc.2006.05.207
M3 - Article
C2 - 16782066
AN - SCOPUS:33745209636
VL - 346
SP - 872
EP - 878
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 3
ER -