TY - JOUR
T1 - Anti-apoptotic function of thymosin-β in developing chick spinal motoneurons
AU - Choi, So Yoen
AU - Kim, Dong Ki
AU - Eun, Bokkee
AU - Kim, Kyungjin
AU - Sun, Woong
AU - Kim, Hyun
N1 - Funding Information:
This work was supported by Korean Ministry of Science and Technology Grants M10412000078-04N1200-07810 (W.S.) and M103KV010018-03K2201-01820 (H.K.) through the 21C frontiers BRC program. We thank Dr. Ronald W Oppenheim (Wake Forest University) and Dr. Dongho Geum (Korea University) for valuable comments.
PY - 2006/8/4
Y1 - 2006/8/4
N2 - Thymosin-βs (Tβs) are water-soluble peptides abundantly present in the cytoplasm and extracellular compartment. The functions of Tβs appear to be pleiotrophic, including actin-remodeling, wound healing, angiogenesis, etc. In the present study, we present the evidence that Tβs have anti-apoptotic activity on developing chick motoneurons (MNs) in vivo. Using in ovo electroporation, we introduced three isoforms of Tβ (Tβ4, Tβ10, and Tβ15) and found the significantly diminished normal and limb bud removal (LBR)-induced programmed cell death. Such anti-apoptotic activity is independent of Tβ's actin remodeling activity. On the other hand, overexpression of Tβs substantially reduced early cell death initiation signal, such as phosphorylation of c-Jun. Collectively, these results suggest that Tβs may prevent apoptosis of neurons via blockade of early apoptogenic signals independent of actin remodeling action.
AB - Thymosin-βs (Tβs) are water-soluble peptides abundantly present in the cytoplasm and extracellular compartment. The functions of Tβs appear to be pleiotrophic, including actin-remodeling, wound healing, angiogenesis, etc. In the present study, we present the evidence that Tβs have anti-apoptotic activity on developing chick motoneurons (MNs) in vivo. Using in ovo electroporation, we introduced three isoforms of Tβ (Tβ4, Tβ10, and Tβ15) and found the significantly diminished normal and limb bud removal (LBR)-induced programmed cell death. Such anti-apoptotic activity is independent of Tβ's actin remodeling activity. On the other hand, overexpression of Tβs substantially reduced early cell death initiation signal, such as phosphorylation of c-Jun. Collectively, these results suggest that Tβs may prevent apoptosis of neurons via blockade of early apoptogenic signals independent of actin remodeling action.
KW - Chick embryo
KW - Jun
KW - Motoneuron
KW - Programmed cell death
KW - Thymosin-β
UR - http://www.scopus.com/inward/record.url?scp=33745209636&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2006.05.207
DO - 10.1016/j.bbrc.2006.05.207
M3 - Article
C2 - 16782066
AN - SCOPUS:33745209636
VL - 346
SP - 872
EP - 878
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -