Anti-apoptotic function of thymosin-β in developing chick spinal motoneurons

So Yoen Choi, Dong Ki Kim, Bokkee Eun, Kyungjin Kim, Woong Sun, Hyun Kim

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Thymosin-βs (Tβs) are water-soluble peptides abundantly present in the cytoplasm and extracellular compartment. The functions of Tβs appear to be pleiotrophic, including actin-remodeling, wound healing, angiogenesis, etc. In the present study, we present the evidence that Tβs have anti-apoptotic activity on developing chick motoneurons (MNs) in vivo. Using in ovo electroporation, we introduced three isoforms of Tβ (Tβ4, Tβ10, and Tβ15) and found the significantly diminished normal and limb bud removal (LBR)-induced programmed cell death. Such anti-apoptotic activity is independent of Tβ's actin remodeling activity. On the other hand, overexpression of Tβs substantially reduced early cell death initiation signal, such as phosphorylation of c-Jun. Collectively, these results suggest that Tβs may prevent apoptosis of neurons via blockade of early apoptogenic signals independent of actin remodeling action.

Original languageEnglish
Pages (from-to)872-878
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume346
Issue number3
DOIs
Publication statusPublished - 2006 Aug 4
Externally publishedYes

Fingerprint

Thymosin
Motor Neurons
Actins
Cell death
Cell Death
Limb Buds
Phosphorylation
Electroporation
Wound Healing
Neurons
Protein Isoforms
Cytoplasm
Apoptosis
Peptides
Water

Keywords

  • Chick embryo
  • Jun
  • Motoneuron
  • Programmed cell death
  • Thymosin-β

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Anti-apoptotic function of thymosin-β in developing chick spinal motoneurons. / Choi, So Yoen; Kim, Dong Ki; Eun, Bokkee; Kim, Kyungjin; Sun, Woong; Kim, Hyun.

In: Biochemical and Biophysical Research Communications, Vol. 346, No. 3, 04.08.2006, p. 872-878.

Research output: Contribution to journalArticle

Choi, So Yoen ; Kim, Dong Ki ; Eun, Bokkee ; Kim, Kyungjin ; Sun, Woong ; Kim, Hyun. / Anti-apoptotic function of thymosin-β in developing chick spinal motoneurons. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 346, No. 3. pp. 872-878.
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