Anti-complementary properties of polysaccharides isolated from fruit bodies of mushroom Pleurotus ostreatus

Mee Hyang Kweon, Hyo Jang, Wang J. Lim, Hyo-Ihl Chang, Chan Wha Kim, Han Chul Yang, Han Joon Hwang, Ha Chin Sung

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A high molecular-weight water-soluble fraction (PO) obtained by the ethanol precipitation of 0.1 N NaOH extracts of the mushroom Pleurotus ostreatus showed 82% anti-complementary activity for complement consumption hemolysis. The PO consisted of 42% carbohydrate (w/w), 50% protein (w/w), and 3% uronic acid (w/w). Fifty-eight percent of the anti-complementary activity decreased by periodate oxidation and 22% by protease digestion, suggesting that the sugar and protein moieties are essential for this activity. Two polysaccharide fractions, PO-IIIa-1 and PO-IIIa-2, with anti-complementary activity were isolated from the PO using DEAE-Sepharose FF followed by Sephadex G75 and Sepharose CL-6B gel permeation chromatographies. The PO- IIIa-2 was found by HPLC to be nearly homogeneous, with the molecular mass of 531 kDa, and showed 96% ITCH50 (inhibition against the total complement hemolysis of deionized water as the control) at a concentration of 1 mg/ml. This fraction contained galactose, mannose, fucose, and glucose with molar ratios of 1.75:1:0.65 and 0.59, respectively. The majority of galactose and mannose units in the PO-IIIa-2 were composed of TGalpl→, →6Galpl→, →2,6Galpl→, and →Manpl→. The PO-IIIa-1 (molecular mass of 2000 kDa), exhibiting higher activity than the PO-IIIa-2, was further purified into two fractions, unbound proteoglycan (PO-IIIa-1A) and bound glucomannan (PO-IIIa- 1B), by affinity chromatography using ConA-Sepharose CL-4B. The anti- complementary activity of each affinity purified fraction decreased as compared to that of the native PO-IIIa-1 fraction, indicating that the formation of complex between both polysaccharide fractions was necessary for full anticomplementary activity.

Original languageEnglish
Pages (from-to)450-456
Number of pages7
JournalJournal of Microbiology and Biotechnology
Volume9
Issue number4
Publication statusPublished - 1999 Aug 1

Fingerprint

Mushroom Bodies
Pleurotus
Molecular mass
Polysaccharides
Mannose
Fruits
Galactose
Fruit
Proteins
Uronic Acids
Affinity chromatography
Fucose
Deionized water
Gel permeation chromatography
Proteoglycans
Carbohydrates
Hemolysis
Sugars
Sepharose
Glucose

Keywords

  • Aggregation
  • Anti-complementary
  • Pleurotus ostreatus
  • Polysaccharides

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Anti-complementary properties of polysaccharides isolated from fruit bodies of mushroom Pleurotus ostreatus. / Kweon, Mee Hyang; Jang, Hyo; Lim, Wang J.; Chang, Hyo-Ihl; Kim, Chan Wha; Yang, Han Chul; Hwang, Han Joon; Sung, Ha Chin.

In: Journal of Microbiology and Biotechnology, Vol. 9, No. 4, 01.08.1999, p. 450-456.

Research output: Contribution to journalArticle

Kweon, Mee Hyang ; Jang, Hyo ; Lim, Wang J. ; Chang, Hyo-Ihl ; Kim, Chan Wha ; Yang, Han Chul ; Hwang, Han Joon ; Sung, Ha Chin. / Anti-complementary properties of polysaccharides isolated from fruit bodies of mushroom Pleurotus ostreatus. In: Journal of Microbiology and Biotechnology. 1999 ; Vol. 9, No. 4. pp. 450-456.
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AU - Chang, Hyo-Ihl

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AU - Yang, Han Chul

AU - Hwang, Han Joon

AU - Sung, Ha Chin

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N2 - A high molecular-weight water-soluble fraction (PO) obtained by the ethanol precipitation of 0.1 N NaOH extracts of the mushroom Pleurotus ostreatus showed 82% anti-complementary activity for complement consumption hemolysis. The PO consisted of 42% carbohydrate (w/w), 50% protein (w/w), and 3% uronic acid (w/w). Fifty-eight percent of the anti-complementary activity decreased by periodate oxidation and 22% by protease digestion, suggesting that the sugar and protein moieties are essential for this activity. Two polysaccharide fractions, PO-IIIa-1 and PO-IIIa-2, with anti-complementary activity were isolated from the PO using DEAE-Sepharose FF followed by Sephadex G75 and Sepharose CL-6B gel permeation chromatographies. The PO- IIIa-2 was found by HPLC to be nearly homogeneous, with the molecular mass of 531 kDa, and showed 96% ITCH50 (inhibition against the total complement hemolysis of deionized water as the control) at a concentration of 1 mg/ml. This fraction contained galactose, mannose, fucose, and glucose with molar ratios of 1.75:1:0.65 and 0.59, respectively. The majority of galactose and mannose units in the PO-IIIa-2 were composed of TGalpl→, →6Galpl→, →2,6Galpl→, and →Manpl→. The PO-IIIa-1 (molecular mass of 2000 kDa), exhibiting higher activity than the PO-IIIa-2, was further purified into two fractions, unbound proteoglycan (PO-IIIa-1A) and bound glucomannan (PO-IIIa- 1B), by affinity chromatography using ConA-Sepharose CL-4B. The anti- complementary activity of each affinity purified fraction decreased as compared to that of the native PO-IIIa-1 fraction, indicating that the formation of complex between both polysaccharide fractions was necessary for full anticomplementary activity.

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