Anti-hyperglycemic activity of polyphenols isolated from barnyard millet (Echinochloa utilis L.) and their role inhibiting α-glucosidase

Kyung Hye Seo, Ji Eun Ra, Sung-Joon Lee, Ji Hae Lee, Sun Rim Kim, Jin Hwan Lee, Woo Duck Seo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The extracts and chemical compounds isolated from barnyard millet (Echinochloa utilis) grains were investigated as part of a search for naturally derived anti-hyperglycemic medicinal plants. Among the five different solvent extracts, the ethyl acetate extract showed the lowest IC<inf>50</inf> value against Saccharomyces cerevisiae α-glucosidase (70.2 μg/mL). Chromatography yielded eight phenolic compounds that may have been responsible for this effect. Among them, N-p-coumaroyl serotonin (1, CS), feruloyl serotonin (2, FS), and luteolin (5) potently inhibited α-glucosidase with IC<inf>50</inf> values of 1.3–17.8 μM compared with those of deoxynojirimycin (DNJ, IC<inf>50</inf> = 2.5 ± 0.1 μM) and acarbose (IC<inf>50</inf> = 255.1 ± 15.6 μM). Additionally, for the first time, we found that CS and FS were significantly inhibited mammalian rat intestinal sucrase (IC<inf>50</inf> of 3.0 and 8.2 μM) and reduced glucose content (73 and 52 % at 0.1 mg/wells) in Caco-2 (human intestinal epithelial) cells. Furthermore, oral glucose tolerance test revealed improved glucose tolerance following treatment with the barnyard millet grains extract by retarding the postprandial rise in blood glucose in vivo. These results suggest that barnyard millet grain can be used as a natural functional medicine to prevent and alleviate type-2 diabetes.

Original languageEnglish
Pages (from-to)571-579
Number of pages9
JournalJournal of the Korean Society for Applied Biological Chemistry
Volume58
Issue number4
DOIs
Publication statusPublished - 2015 Aug 30

Fingerprint

Echinochloa
Glucosidases
Polyphenols
Inhibitory Concentration 50
Glucose
Acarbose
Sucrase
Luteolin
Chemical compounds
Medical problems
Chromatography
Yeast
Medicine
Blood Glucose
Rats
Serotonin
Medicinal Plants
Glucose Tolerance Test
Type 2 Diabetes Mellitus
Saccharomyces cerevisiae

Keywords

  • Anti-hyperglycemic
  • Barnyard millet grains
  • Caco-2 cells
  • Serotonin
  • α-Glucosidase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Organic Chemistry

Cite this

Anti-hyperglycemic activity of polyphenols isolated from barnyard millet (Echinochloa utilis L.) and their role inhibiting α-glucosidase. / Seo, Kyung Hye; Ra, Ji Eun; Lee, Sung-Joon; Lee, Ji Hae; Kim, Sun Rim; Lee, Jin Hwan; Seo, Woo Duck.

In: Journal of the Korean Society for Applied Biological Chemistry, Vol. 58, No. 4, 30.08.2015, p. 571-579.

Research output: Contribution to journalArticle

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abstract = "The extracts and chemical compounds isolated from barnyard millet (Echinochloa utilis) grains were investigated as part of a search for naturally derived anti-hyperglycemic medicinal plants. Among the five different solvent extracts, the ethyl acetate extract showed the lowest IC50 value against Saccharomyces cerevisiae α-glucosidase (70.2 μg/mL). Chromatography yielded eight phenolic compounds that may have been responsible for this effect. Among them, N-p-coumaroyl serotonin (1, CS), feruloyl serotonin (2, FS), and luteolin (5) potently inhibited α-glucosidase with IC50 values of 1.3–17.8 μM compared with those of deoxynojirimycin (DNJ, IC50 = 2.5 ± 0.1 μM) and acarbose (IC50 = 255.1 ± 15.6 μM). Additionally, for the first time, we found that CS and FS were significantly inhibited mammalian rat intestinal sucrase (IC50 of 3.0 and 8.2 μM) and reduced glucose content (73 and 52 {\%} at 0.1 mg/wells) in Caco-2 (human intestinal epithelial) cells. Furthermore, oral glucose tolerance test revealed improved glucose tolerance following treatment with the barnyard millet grains extract by retarding the postprandial rise in blood glucose in vivo. These results suggest that barnyard millet grain can be used as a natural functional medicine to prevent and alleviate type-2 diabetes.",
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