Anti-inflammatory activity of p-coumaryl alcohol-γ-O-methyl ether is mediated through modulation of interferon-γ production in Th cells

E. S. Yu, H. J. Min, K. Lee, M. S. Lee, J. W. Nam, E. K. Seo, Jeong-Ho Hong, E. S. Hwang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background and purpose: p-Coumaryl alcohol-γ-O-methyl ether (CAME) was isolated from Alpinia galanga and shown to contain a phenylpropanoid structure similar to p-coumaryl diacetate (CDA). CDA is known to have antioxidant and anti-inflammatory activity, but the biochemical activities of CAME are unknown. Inflammation is mediated by inflammatory cytokine production, in particular, by CD4+ T helper cells (Th cells), but it is unclear whether phenylpropanoids affect cytokine production in Th cells. In this study, we decided to investigate the functions of CAME and CDA in CD4+ Th cells. Experimental approach: Mouse CD4+ Th cells were isolated from C57BL6 mice and stimulated with an antibody against T cell receptors in the presence of phenylpropanoids. Cytokine production was measured by elisa and intracellular cytokine staining. Gene knockout mice and tetracycline-inducible transgenic mice were used to examine the molecular mechanisms of phenylpropanoids on modulation of cytokine production. Key results: CAME potently reduced intracellular reactive oxygen species in Th cells, as does CDA. However, although CDA was cytotoxic, CAME selectively and potently suppresses interferon-γ (IFNγ) production in CD4+ Th cells, without toxicity. This effect was caused by attenuated expression of the transcription factor, T-box protein expressed in T cells (T-bet), and T-bet was essential for CAME to inhibit IFNγ production in CD4+ Th cells. Conclusions and implications: CAME selectively and substantially suppresses IFNγ production in CD4+ Th cells by decreasing T-bet expression. As increased IFNγ production by CD4+ Th cells can mediate inflammatory immune responses, a selective IFNγ suppressor, such as CAME may be an effective, naturally occurring, compound for modulating inflammatory immune disorders.

Original languageEnglish
Pages (from-to)1107-1114
Number of pages8
JournalBritish Journal of Pharmacology
Volume156
Issue number7
DOIs
Publication statusPublished - 2009 Apr 1

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Methyl Ethers
Helper-Inducer T-Lymphocytes
Interferons
Anti-Inflammatory Agents
Alcohols
Cytokines
T-Lymphocytes
Alpinia
trans-3-(4'-hydroxyphenyl)-2-propenoic acid
Gene Knockout Techniques
Proteins
Immune System Diseases
T-Cell Antigen Receptor
Tetracycline
Knockout Mice
Transgenic Mice
Reactive Oxygen Species
Transcription Factors
Antioxidants
Staining and Labeling

Keywords

  • Anti-inflammatory activity
  • Antioxidant
  • CD4+ Th cells
  • IFNγ
  • P-coumaryl alcohol-γ-O-methyl ether
  • P-coumaryl diacetate
  • Phenylpropanoids
  • T-bet

ASJC Scopus subject areas

  • Pharmacology

Cite this

Anti-inflammatory activity of p-coumaryl alcohol-γ-O-methyl ether is mediated through modulation of interferon-γ production in Th cells. / Yu, E. S.; Min, H. J.; Lee, K.; Lee, M. S.; Nam, J. W.; Seo, E. K.; Hong, Jeong-Ho; Hwang, E. S.

In: British Journal of Pharmacology, Vol. 156, No. 7, 01.04.2009, p. 1107-1114.

Research output: Contribution to journalArticle

Yu, E. S. ; Min, H. J. ; Lee, K. ; Lee, M. S. ; Nam, J. W. ; Seo, E. K. ; Hong, Jeong-Ho ; Hwang, E. S. / Anti-inflammatory activity of p-coumaryl alcohol-γ-O-methyl ether is mediated through modulation of interferon-γ production in Th cells. In: British Journal of Pharmacology. 2009 ; Vol. 156, No. 7. pp. 1107-1114.
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AU - Lee, M. S.

AU - Nam, J. W.

AU - Seo, E. K.

AU - Hong, Jeong-Ho

AU - Hwang, E. S.

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KW - T-bet

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