Anti-inflammatory effects of mesenchymal stem cell-derived exosomal microRNA-146a-5p and microRNA-548e-5p on human trophoblast cells

Changwon Yang, Whasun Lim, Junghyun Park, Sunwoo Park, Seungkwon You, Gwonhwa Song

Research output: Contribution to journalArticle

Abstract

Human umbilical cord mesenchymal stem cells (MSCs) have been reported to improve the migration and invasion of trophoblast cells; however, little is known about whether MSC-derived exosomes and exosomal miRNAs can regulate trophoblast cell properties. In this study, we investigated whether exosomal miRNAs from amniotic fluid-derived MSC (AF-MSC) could regulate the inflammatory response of the human trophoblast cell line HTR8/SVneo. We verified the anti-inflammatory effects of AF-MSCs on lipopolysaccharide (LPS)-induced inflammatory trophoblast cells and found that miR-146a-5p and miR-548e-5p in the AF-MSC-derived exosomes regulate nuclear factor κB, AKT and mitogen-activated protein kinase protein phosphorylation. Furthermore, we found that the transfection of human trophoblast cells with miR-146a-5p and miR-548e-5p inhibitors reduced trophoblast migration (P < 0.05 vs control) and the expression of proliferating cell nuclear antigen, a protein essential for cell proliferation (P < 0.01 vs control). In particular, the miR-548e-5p inhibitor induced apoptosis, while tumor necrosis factor receptor-associated factor 6, a predicted target of miR-146a-5p and miR-548e-5p, was involved in the regulation of oxidative stress in the human trophoblast cells. In a mouse model of LPS-induced preterm birth (PB), miR-146a-5p expression was found to be relatively low in the group in which the effect of AF-MSCs was insignificant. However, this study is limited in that the changes in the expression of some genes in response to AF-MSCs differ between the cell line and mouse model. Collectively, these data show that exosomal miR-146a-5p and miR-548e-5p from AF-MSCs have anti-inflammatory effects on human trophoblast cells and may be novel targets for treating inflammatory diseases and associated problems that occur during pregnancy, such as PB.

Original languageEnglish
Pages (from-to)755-771
Number of pages17
JournalMolecular human reproduction
Volume25
Issue number11
DOIs
Publication statusPublished - 2019 Nov 30

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Trophoblasts
MicroRNAs
Mesenchymal Stromal Cells
Anti-Inflammatory Agents
Exosomes
Premature Birth
Amniotic Fluid
Lipopolysaccharides
Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
Cell Line
Umbilical Cord
Proliferating Cell Nuclear Antigen
Mitogen-Activated Protein Kinases
Transfection
Proteins
Oxidative Stress
Phosphorylation
Cell Proliferation
Apoptosis
Gene Expression

Keywords

  • exosome
  • inflammation
  • lipopolysaccharide
  • migration
  • nuclear factor κB
  • preterm birth
  • trophoblast
  • tumor necrosis factor receptor associated factor 6

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Cell Biology

Cite this

Anti-inflammatory effects of mesenchymal stem cell-derived exosomal microRNA-146a-5p and microRNA-548e-5p on human trophoblast cells. / Yang, Changwon; Lim, Whasun; Park, Junghyun; Park, Sunwoo; You, Seungkwon; Song, Gwonhwa.

In: Molecular human reproduction, Vol. 25, No. 11, 30.11.2019, p. 755-771.

Research output: Contribution to journalArticle

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