Anti-Inflammatory Effects of Stearidonic Acid Mediated by Suppression of NF-κB and MAP-Kinase Pathways in Macrophages

Jeehye Sung, Heemang Jeon, In-Hwan Kim, Heon Sang Jeong, Junsoo Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Stearidonic acid (SDA, 18:4n-3) is an omega-3 polyunsaturated fatty acid present in oils derived from plants of the Boraginaceae family. In this study, we determined the anti-inflammatory effects of SDA isolated from echium oil on lipopolysaccharide (LPS)-induced inflammatory responses in RAW 264.7 macrophages. SDA significantly downregulated the levels of the inducible nitric oxide synthase (iNOS) protein, thereby suppressing the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. In addition, SDA inhibited the nuclear translocation and promoter activity of nuclear factor κB (NFκB) and the phosphorylation of mitogen-activated protein kinases (MAPK) such as extracellular signal regulated kinase 1/2, c-jun N terminal kinase, and p38 in LPS-stimulated RAW 264.7 cells. Our results showed that SDA exerted anti-inflammatory effects by suppressing iNOS-mediated NO production via inactivation of NFκB and MAPK signaling pathways.

Original languageEnglish
Pages (from-to)781-787
Number of pages7
JournalLipids
Volume52
Issue number9
DOIs
Publication statusPublished - 2017 Sep 1

Fingerprint

Macrophages
Lipopolysaccharides
Anti-Inflammatory Agents
Phosphotransferases
Nitric Oxide Synthase Type II
Mitogen-Activated Protein Kinases
Echium
Nitric Oxide
Oils
Mitogen-Activated Protein Kinase 9
Boraginaceae
Phosphorylation
Mitogen-Activated Protein Kinase 3
Plant Oils
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinase 1
Omega-3 Fatty Acids
Unsaturated Fatty Acids
Down-Regulation
stearidonic acid

Keywords

  • Anti-inflammatory
  • Echium oil
  • MAPK (mitogen-activated protein kinases) pathway
  • NFκB (nuclear factor κB)
  • Stearidonic acid

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Cite this

Anti-Inflammatory Effects of Stearidonic Acid Mediated by Suppression of NF-κB and MAP-Kinase Pathways in Macrophages. / Sung, Jeehye; Jeon, Heemang; Kim, In-Hwan; Jeong, Heon Sang; Lee, Junsoo.

In: Lipids, Vol. 52, No. 9, 01.09.2017, p. 781-787.

Research output: Contribution to journalArticle

Sung, Jeehye ; Jeon, Heemang ; Kim, In-Hwan ; Jeong, Heon Sang ; Lee, Junsoo. / Anti-Inflammatory Effects of Stearidonic Acid Mediated by Suppression of NF-κB and MAP-Kinase Pathways in Macrophages. In: Lipids. 2017 ; Vol. 52, No. 9. pp. 781-787.
@article{549c99db64e14b679a1fbbcec0693a47,
title = "Anti-Inflammatory Effects of Stearidonic Acid Mediated by Suppression of NF-κB and MAP-Kinase Pathways in Macrophages",
abstract = "Stearidonic acid (SDA, 18:4n-3) is an omega-3 polyunsaturated fatty acid present in oils derived from plants of the Boraginaceae family. In this study, we determined the anti-inflammatory effects of SDA isolated from echium oil on lipopolysaccharide (LPS)-induced inflammatory responses in RAW 264.7 macrophages. SDA significantly downregulated the levels of the inducible nitric oxide synthase (iNOS) protein, thereby suppressing the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. In addition, SDA inhibited the nuclear translocation and promoter activity of nuclear factor κB (NFκB) and the phosphorylation of mitogen-activated protein kinases (MAPK) such as extracellular signal regulated kinase 1/2, c-jun N terminal kinase, and p38 in LPS-stimulated RAW 264.7 cells. Our results showed that SDA exerted anti-inflammatory effects by suppressing iNOS-mediated NO production via inactivation of NFκB and MAPK signaling pathways.",
keywords = "Anti-inflammatory, Echium oil, MAPK (mitogen-activated protein kinases) pathway, NFκB (nuclear factor κB), Stearidonic acid",
author = "Jeehye Sung and Heemang Jeon and In-Hwan Kim and Jeong, {Heon Sang} and Junsoo Lee",
year = "2017",
month = "9",
day = "1",
doi = "10.1007/s11745-017-4278-6",
language = "English",
volume = "52",
pages = "781--787",
journal = "Lipids",
issn = "0024-4201",
publisher = "Springer Verlag",
number = "9",

}

TY - JOUR

T1 - Anti-Inflammatory Effects of Stearidonic Acid Mediated by Suppression of NF-κB and MAP-Kinase Pathways in Macrophages

AU - Sung, Jeehye

AU - Jeon, Heemang

AU - Kim, In-Hwan

AU - Jeong, Heon Sang

AU - Lee, Junsoo

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Stearidonic acid (SDA, 18:4n-3) is an omega-3 polyunsaturated fatty acid present in oils derived from plants of the Boraginaceae family. In this study, we determined the anti-inflammatory effects of SDA isolated from echium oil on lipopolysaccharide (LPS)-induced inflammatory responses in RAW 264.7 macrophages. SDA significantly downregulated the levels of the inducible nitric oxide synthase (iNOS) protein, thereby suppressing the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. In addition, SDA inhibited the nuclear translocation and promoter activity of nuclear factor κB (NFκB) and the phosphorylation of mitogen-activated protein kinases (MAPK) such as extracellular signal regulated kinase 1/2, c-jun N terminal kinase, and p38 in LPS-stimulated RAW 264.7 cells. Our results showed that SDA exerted anti-inflammatory effects by suppressing iNOS-mediated NO production via inactivation of NFκB and MAPK signaling pathways.

AB - Stearidonic acid (SDA, 18:4n-3) is an omega-3 polyunsaturated fatty acid present in oils derived from plants of the Boraginaceae family. In this study, we determined the anti-inflammatory effects of SDA isolated from echium oil on lipopolysaccharide (LPS)-induced inflammatory responses in RAW 264.7 macrophages. SDA significantly downregulated the levels of the inducible nitric oxide synthase (iNOS) protein, thereby suppressing the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. In addition, SDA inhibited the nuclear translocation and promoter activity of nuclear factor κB (NFκB) and the phosphorylation of mitogen-activated protein kinases (MAPK) such as extracellular signal regulated kinase 1/2, c-jun N terminal kinase, and p38 in LPS-stimulated RAW 264.7 cells. Our results showed that SDA exerted anti-inflammatory effects by suppressing iNOS-mediated NO production via inactivation of NFκB and MAPK signaling pathways.

KW - Anti-inflammatory

KW - Echium oil

KW - MAPK (mitogen-activated protein kinases) pathway

KW - NFκB (nuclear factor κB)

KW - Stearidonic acid

UR - http://www.scopus.com/inward/record.url?scp=85025821810&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85025821810&partnerID=8YFLogxK

U2 - 10.1007/s11745-017-4278-6

DO - 10.1007/s11745-017-4278-6

M3 - Article

C2 - 28744771

AN - SCOPUS:85025821810

VL - 52

SP - 781

EP - 787

JO - Lipids

JF - Lipids

SN - 0024-4201

IS - 9

ER -