Abstract
Stearidonic acid (SDA, 18:4n-3) is an omega-3 polyunsaturated fatty acid present in oils derived from plants of the Boraginaceae family. In this study, we determined the anti-inflammatory effects of SDA isolated from echium oil on lipopolysaccharide (LPS)-induced inflammatory responses in RAW 264.7 macrophages. SDA significantly downregulated the levels of the inducible nitric oxide synthase (iNOS) protein, thereby suppressing the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. In addition, SDA inhibited the nuclear translocation and promoter activity of nuclear factor κB (NFκB) and the phosphorylation of mitogen-activated protein kinases (MAPK) such as extracellular signal regulated kinase 1/2, c-jun N terminal kinase, and p38 in LPS-stimulated RAW 264.7 cells. Our results showed that SDA exerted anti-inflammatory effects by suppressing iNOS-mediated NO production via inactivation of NFκB and MAPK signaling pathways.
| Original language | English |
|---|---|
| Pages (from-to) | 781-787 |
| Number of pages | 7 |
| Journal | Lipids |
| Volume | 52 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2017 Sep 1 |
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Keywords
- Anti-inflammatory
- Echium oil
- MAPK (mitogen-activated protein kinases) pathway
- NFκB (nuclear factor κB)
- Stearidonic acid
ASJC Scopus subject areas
- Biochemistry
- Organic Chemistry
- Cell Biology
Cite this
Anti-Inflammatory Effects of Stearidonic Acid Mediated by Suppression of NF-κB and MAP-Kinase Pathways in Macrophages. / Sung, Jeehye; Jeon, Heemang; Kim, In-Hwan; Jeong, Heon Sang; Lee, Junsoo.
In: Lipids, Vol. 52, No. 9, 01.09.2017, p. 781-787.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anti-Inflammatory Effects of Stearidonic Acid Mediated by Suppression of NF-κB and MAP-Kinase Pathways in Macrophages
AU - Sung, Jeehye
AU - Jeon, Heemang
AU - Kim, In-Hwan
AU - Jeong, Heon Sang
AU - Lee, Junsoo
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Stearidonic acid (SDA, 18:4n-3) is an omega-3 polyunsaturated fatty acid present in oils derived from plants of the Boraginaceae family. In this study, we determined the anti-inflammatory effects of SDA isolated from echium oil on lipopolysaccharide (LPS)-induced inflammatory responses in RAW 264.7 macrophages. SDA significantly downregulated the levels of the inducible nitric oxide synthase (iNOS) protein, thereby suppressing the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. In addition, SDA inhibited the nuclear translocation and promoter activity of nuclear factor κB (NFκB) and the phosphorylation of mitogen-activated protein kinases (MAPK) such as extracellular signal regulated kinase 1/2, c-jun N terminal kinase, and p38 in LPS-stimulated RAW 264.7 cells. Our results showed that SDA exerted anti-inflammatory effects by suppressing iNOS-mediated NO production via inactivation of NFκB and MAPK signaling pathways.
AB - Stearidonic acid (SDA, 18:4n-3) is an omega-3 polyunsaturated fatty acid present in oils derived from plants of the Boraginaceae family. In this study, we determined the anti-inflammatory effects of SDA isolated from echium oil on lipopolysaccharide (LPS)-induced inflammatory responses in RAW 264.7 macrophages. SDA significantly downregulated the levels of the inducible nitric oxide synthase (iNOS) protein, thereby suppressing the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. In addition, SDA inhibited the nuclear translocation and promoter activity of nuclear factor κB (NFκB) and the phosphorylation of mitogen-activated protein kinases (MAPK) such as extracellular signal regulated kinase 1/2, c-jun N terminal kinase, and p38 in LPS-stimulated RAW 264.7 cells. Our results showed that SDA exerted anti-inflammatory effects by suppressing iNOS-mediated NO production via inactivation of NFκB and MAPK signaling pathways.
KW - Anti-inflammatory
KW - Echium oil
KW - MAPK (mitogen-activated protein kinases) pathway
KW - NFκB (nuclear factor κB)
KW - Stearidonic acid
UR - http://www.scopus.com/inward/record.url?scp=85025821810&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85025821810&partnerID=8YFLogxK
U2 - 10.1007/s11745-017-4278-6
DO - 10.1007/s11745-017-4278-6
M3 - Article
C2 - 28744771
AN - SCOPUS:85025821810
VL - 52
SP - 781
EP - 787
JO - Lipids
JF - Lipids
SN - 0024-4201
IS - 9
ER -