Anti-inflammatory potential of a heat-killed Lactobacillus strain isolated from Kimchi on house dust mite-induced atopic dermatitis in NC/Nga mice

C. Y. Choi, Y. H. Kim, S. Oh, H. J. Lee, J. H. Kim, S. H. Park, H. J. Kim, S. J. Lee, Taehoon Chun

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aims: Atopic dermatitis (AD) is an allergic skin disease driven by the Th2-prone immune response. Therefore, a fundamental approach to restoring the Th1/Th2 balance is needed to treat AD. Methods and Results: Eighteen different Lactobacillus strains isolated from Kimchi were screened to identify those that stimulated immune cells to secret Th1-type or Th2-type cytokines. Lactobacillus brevis NS1401 induced the greatest IFN-γ and IL-12 secretion and the least IL-4 production among the tested Lactobacillus strains. Furthermore, oral administration of heat-killed NS1401 ameliorated the symptoms of dust mite-induced AD in NC/Nga mice by decreasing the serum IgE level and reducing the number of mast cells and eosinophils in lesions. Also, the size and number of cells in the draining lymph nodes of NS1401-administered mice were significantly reduced. In agreement with these results, secretion of a Th1-type cytokine (IFN-γ) and allergen-specific IgG2a were increased, whereas secretion of Th2-type cytokines (IL-4, IL-5, and IL-10) and allergen-specific IgG1 were decreased upon administration of NS1401 in mice. Conclusions: Lactobacillus brevis NS1401 alleviates the symptoms of AD by restoring the Th1/Th2 balance through enhancing Th1-prone immunity. Significance and Impact of the Study: The immunomodulatory function of L. brevis NS1401 may provide effective new therapeutics against AD.

Original languageEnglish
Pages (from-to)535-543
Number of pages9
JournalJournal of Applied Microbiology
Volume123
Issue number2
DOIs
Publication statusPublished - 2017 Aug 1

Fingerprint

Pyroglyphidae
Lactobacillus
Atopic Dermatitis
Anti-Inflammatory Agents
Hot Temperature
Lactobacillus brevis
Th1-Th2 Balance
Cytokines
Interleukin-4
Allergens
Mites
Interleukin-5
Interleukin-12
Dust
Cell Size
Skin Diseases
Eosinophils
Mast Cells
Interleukin-10
Immunoglobulin E

Keywords

  • atopic dermatitis
  • house dust mite
  • Kimchi
  • Lactobacillus
  • NC/Nga mouse

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Anti-inflammatory potential of a heat-killed Lactobacillus strain isolated from Kimchi on house dust mite-induced atopic dermatitis in NC/Nga mice. / Choi, C. Y.; Kim, Y. H.; Oh, S.; Lee, H. J.; Kim, J. H.; Park, S. H.; Kim, H. J.; Lee, S. J.; Chun, Taehoon.

In: Journal of Applied Microbiology, Vol. 123, No. 2, 01.08.2017, p. 535-543.

Research output: Contribution to journalArticle

Choi, C. Y. ; Kim, Y. H. ; Oh, S. ; Lee, H. J. ; Kim, J. H. ; Park, S. H. ; Kim, H. J. ; Lee, S. J. ; Chun, Taehoon. / Anti-inflammatory potential of a heat-killed Lactobacillus strain isolated from Kimchi on house dust mite-induced atopic dermatitis in NC/Nga mice. In: Journal of Applied Microbiology. 2017 ; Vol. 123, No. 2. pp. 535-543.
@article{ed21fd4091864d80be2035798ae5aba0,
title = "Anti-inflammatory potential of a heat-killed Lactobacillus strain isolated from Kimchi on house dust mite-induced atopic dermatitis in NC/Nga mice",
abstract = "Aims: Atopic dermatitis (AD) is an allergic skin disease driven by the Th2-prone immune response. Therefore, a fundamental approach to restoring the Th1/Th2 balance is needed to treat AD. Methods and Results: Eighteen different Lactobacillus strains isolated from Kimchi were screened to identify those that stimulated immune cells to secret Th1-type or Th2-type cytokines. Lactobacillus brevis NS1401 induced the greatest IFN-γ and IL-12 secretion and the least IL-4 production among the tested Lactobacillus strains. Furthermore, oral administration of heat-killed NS1401 ameliorated the symptoms of dust mite-induced AD in NC/Nga mice by decreasing the serum IgE level and reducing the number of mast cells and eosinophils in lesions. Also, the size and number of cells in the draining lymph nodes of NS1401-administered mice were significantly reduced. In agreement with these results, secretion of a Th1-type cytokine (IFN-γ) and allergen-specific IgG2a were increased, whereas secretion of Th2-type cytokines (IL-4, IL-5, and IL-10) and allergen-specific IgG1 were decreased upon administration of NS1401 in mice. Conclusions: Lactobacillus brevis NS1401 alleviates the symptoms of AD by restoring the Th1/Th2 balance through enhancing Th1-prone immunity. Significance and Impact of the Study: The immunomodulatory function of L. brevis NS1401 may provide effective new therapeutics against AD.",
keywords = "atopic dermatitis, house dust mite, Kimchi, Lactobacillus, NC/Nga mouse",
author = "Choi, {C. Y.} and Kim, {Y. H.} and S. Oh and Lee, {H. J.} and Kim, {J. H.} and Park, {S. H.} and Kim, {H. J.} and Lee, {S. J.} and Taehoon Chun",
year = "2017",
month = "8",
day = "1",
doi = "10.1111/jam.13515",
language = "English",
volume = "123",
pages = "535--543",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "2",

}

TY - JOUR

T1 - Anti-inflammatory potential of a heat-killed Lactobacillus strain isolated from Kimchi on house dust mite-induced atopic dermatitis in NC/Nga mice

AU - Choi, C. Y.

AU - Kim, Y. H.

AU - Oh, S.

AU - Lee, H. J.

AU - Kim, J. H.

AU - Park, S. H.

AU - Kim, H. J.

AU - Lee, S. J.

AU - Chun, Taehoon

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Aims: Atopic dermatitis (AD) is an allergic skin disease driven by the Th2-prone immune response. Therefore, a fundamental approach to restoring the Th1/Th2 balance is needed to treat AD. Methods and Results: Eighteen different Lactobacillus strains isolated from Kimchi were screened to identify those that stimulated immune cells to secret Th1-type or Th2-type cytokines. Lactobacillus brevis NS1401 induced the greatest IFN-γ and IL-12 secretion and the least IL-4 production among the tested Lactobacillus strains. Furthermore, oral administration of heat-killed NS1401 ameliorated the symptoms of dust mite-induced AD in NC/Nga mice by decreasing the serum IgE level and reducing the number of mast cells and eosinophils in lesions. Also, the size and number of cells in the draining lymph nodes of NS1401-administered mice were significantly reduced. In agreement with these results, secretion of a Th1-type cytokine (IFN-γ) and allergen-specific IgG2a were increased, whereas secretion of Th2-type cytokines (IL-4, IL-5, and IL-10) and allergen-specific IgG1 were decreased upon administration of NS1401 in mice. Conclusions: Lactobacillus brevis NS1401 alleviates the symptoms of AD by restoring the Th1/Th2 balance through enhancing Th1-prone immunity. Significance and Impact of the Study: The immunomodulatory function of L. brevis NS1401 may provide effective new therapeutics against AD.

AB - Aims: Atopic dermatitis (AD) is an allergic skin disease driven by the Th2-prone immune response. Therefore, a fundamental approach to restoring the Th1/Th2 balance is needed to treat AD. Methods and Results: Eighteen different Lactobacillus strains isolated from Kimchi were screened to identify those that stimulated immune cells to secret Th1-type or Th2-type cytokines. Lactobacillus brevis NS1401 induced the greatest IFN-γ and IL-12 secretion and the least IL-4 production among the tested Lactobacillus strains. Furthermore, oral administration of heat-killed NS1401 ameliorated the symptoms of dust mite-induced AD in NC/Nga mice by decreasing the serum IgE level and reducing the number of mast cells and eosinophils in lesions. Also, the size and number of cells in the draining lymph nodes of NS1401-administered mice were significantly reduced. In agreement with these results, secretion of a Th1-type cytokine (IFN-γ) and allergen-specific IgG2a were increased, whereas secretion of Th2-type cytokines (IL-4, IL-5, and IL-10) and allergen-specific IgG1 were decreased upon administration of NS1401 in mice. Conclusions: Lactobacillus brevis NS1401 alleviates the symptoms of AD by restoring the Th1/Th2 balance through enhancing Th1-prone immunity. Significance and Impact of the Study: The immunomodulatory function of L. brevis NS1401 may provide effective new therapeutics against AD.

KW - atopic dermatitis

KW - house dust mite

KW - Kimchi

KW - Lactobacillus

KW - NC/Nga mouse

UR - http://www.scopus.com/inward/record.url?scp=85023203774&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85023203774&partnerID=8YFLogxK

U2 - 10.1111/jam.13515

DO - 10.1111/jam.13515

M3 - Article

VL - 123

SP - 535

EP - 543

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 2

ER -