Anti-inflammatory withanolides from the aerial parts of Physalis minima

Wenhui Liu, Han Zhang, Hongxu Wan, Jiantong Hou, Dongho Lee, Jing Xu, Yuanqiang Guo

Research output: Contribution to journalArticlepeer-review

Abstract

Eight undescribed and two known withanolides were obtained from the aerial parts of Physalis minima. The structures of these compounds were defined by spectroscopic methods including 1D and 2D NMR, HRESIMS, and electronic circular dichroism (ECD) data analysis. Physminin E was elucidated to be a rare 13,14-seco-withanolide. Inhibitory effects of these compounds on nitric oxide (NO) production were evaluated by using LPS-activated RAW264.7 macrophages, and physminin C was shown to be the most active with an IC50 value of 3.5 μM. The further mechanistic analysis of NO inhibition was performed by molecular docking and Western blotting.

Original languageEnglish
Article number113301
JournalPhytochemistry
Volume202
DOIs
Publication statusPublished - 2022 Oct

Keywords

  • Molecular docking
  • Nitric oxide inhibitory activities
  • Physalis minima
  • Solanaceae
  • Withanolides

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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