Anti-obesity effects of germinated brown rice extract through down-regulation of lipogenic genes in high fat diet-induced obese mice

Jin Nyoung Ho, Mi Eun Son, Won Chul Lim, Seung Taik Lim, Hong Yon Cho

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Lipid accumulation using Oil Red O dye was measured in 3T3-L1 murine adipocytes to examine the anti-obesity effect of four types of germinated rice, including germinated brown rice (GBR), germinated waxy brown rice (GWBR), germinated black rice (GBR), and germinated waxy black rice (GWB-R). GBR methanol extract exhibited the highest suppression of lipid accumulation in the 3T3-L1 cell line and also the anti-obesity effect of GBR on high fat induced-obese mice. The mice were divided into three groups and were administered: ND, a normal diet; HFD control, a high fat diet; and GBR, a high fat diet plus 0.15% GBR methanol extract for 7 weeks. GBR administration significantly decreased body weight gain and lipid accumulation in the liver and epididymal adipose tissue as compared to the HFD control group. In addition, serum triglycerides (TGs) and total cholesterol (TC) levels were significantly decreased by following GBR administration compared with those in the HFD control group, whereas the high-density lipoprotein (HDL) cholesterol level increased. Furthermore, the mRNA levels of adipogenic transcriptional factors, such as CCAAT enhancer binding protein (C/EBP)-α, sterol regulatory element-binding protein (SREBP)-1c, and peroxisome proliferator activated receptors (PPAR)-γ, and related genes (aP2, FAS), decreased significantly. Taken together, GBR administration suppressed body weight gain and lipid accumulation in the liver and epididymal adipocytes, and improved serum lipid profiles, in part, by controlling adipogenesis through a reduction in transcriptional factors. These results suggest that GBR is a potential agent against obesity.

Original languageEnglish
Pages (from-to)1068-1074
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number6
DOIs
Publication statusPublished - 2012 Jun 29

Fingerprint

Obese Mice
High Fat Diet
Nutrition
Down-Regulation
Obesity
Genes
Fats
Lipids
Liver
Methanol
Sterol Regulatory Element Binding Protein 1
CCAAT-Enhancer-Binding Proteins
Peroxisome Proliferator-Activated Receptors
HDL Cholesterol
Triglycerides
Coloring Agents
Adipocytes
Cholesterol
Cells
Oryza

Keywords

  • 3T3-L1
  • Anti-obesity
  • Germinated brown rice
  • High fatinduced obese mice

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Anti-obesity effects of germinated brown rice extract through down-regulation of lipogenic genes in high fat diet-induced obese mice. / Ho, Jin Nyoung; Son, Mi Eun; Lim, Won Chul; Lim, Seung Taik; Cho, Hong Yon.

In: Bioscience, Biotechnology and Biochemistry, Vol. 76, No. 6, 29.06.2012, p. 1068-1074.

Research output: Contribution to journalArticle

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