Anti-obesity effects of isoacteoside on 3T3-L1 adipocytes

Chang Geun Choi, Deok Jae Lee, Namhyun Chung, Yong Hoon Joo

Research output: Contribution to journalArticlepeer-review

Abstract

Isoacteoside is a caffeoyl phenylethanoide glycoside found in various plant parts, such as the flower of Magnolia denudata. In particular, magnolia has been studied for its anti-obesity, anticancer, and anti-inflammatory effects. However, isoacteoside has not been extensively studied, except for its anti-inflammatory effects. In this study, the anti-obesity effects of isoacteoside were investigated in 3T3-L1 mouse pre-adipocytes. Isoacteoside treatment did not induce cytotoxicity in 3T3-L1 cells up to a concentration of 1000 μM. The anti-obesity effects on 3T3-L1 cells were confirmed using oil red O staining. In addition, the expression of obesity-related proteins and genes, such as peroxisome proliferator-activated acceptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), and perilipin (PLIN1), was determined by western blotting and qRT-PCR assays to confirm the anti-obesity effects of isoacteoside. The results of this study suggest that isoacteoside, a natural substance isolated from plant extracts, is not highly toxic to normal cells, such as pre-adipocytes, and displays anti-obesity effects in vitro.

Original languageEnglish
Article number33
JournalApplied Biological Chemistry
Volume65
Issue number1
DOIs
Publication statusPublished - 2022 Dec

Keywords

  • 3T3-L1 cells
  • Anti-obesity
  • Isoacteoside
  • Oil Red O assay
  • Phenylethanoid glycosides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Organic Chemistry

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