Anti-oxidative effect of some plant extracts against nitric oxide-induced oxidative stress on neuronal cell

Uk Koo, Hak Ju Lee, Dong Ho Lee, Hyun Jung Lee, Ah Rom Ham, Eun Young Cho, Woongchon Mar

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The objective of this study is screening the anti-oxidative effects of several plant MeOH extracts against oxidative stress in Neuroblastoma cell. Oxidative stress has been implicated in the pathogenesis of many neurotoxicity, neurodegenerative disorders and cell death. This oxidative stress is generated by ROS (Reactive Oxygen Species) such as nitric oxide, nitrogen dioxide, peroxyl, superoxide (O2-), hydroxyl, alkoxyl. So, in the present study, we induced oxidative stress by treatment of sodium nitroprusside (2.5 mM) in human neuroblastoma SH-SY5Y cell which was treated samples before 24hr, and cell viability was measured by MTT reduction assay. Of those tested, the extracts of Paeonia japonica (roots), Eucommia ulmoides ()(barks), Paeonia japonica ()(roots), Phyllostachys bambusoides (stems), Polygala tenuifolia ()(roots), Paeonia japonica (roots), Polygala tenuifolia (roots), Machilus thunbergii (barks), Mallotus japonicus (leaves), Poria cocos (whole), Sophora flavescens (roots), Angelica tenuissima (roots), Angelica gigas ()(roots) showed anti-oxidative effects[EC50<15.20 μg/ml(Carnosine:Positive control)]in dose dependent manner.

Original languageEnglish
Pages (from-to)290-294
Number of pages5
JournalKorean Journal of Pharmacognosy
Volume39
Issue number4
Publication statusPublished - 2008 Dec 1

Keywords

  • Anti-oxidative effects
  • EC
  • MTT reduction assay
  • Oxidative stress
  • Sodium nitroprusside

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery

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