Anti-Siglec-F antibody inhibits oral egg allergen induced intestinal eosinophilic inflammation in a mouse model

Dae Jin Song, Jae Youn Cho, Marina Miller, Wendy Strangman, Mai Zhang, Ajit Varki, David H. Broide

    Research output: Contribution to journalArticlepeer-review

    57 Citations (Scopus)

    Abstract

    Siglec-F is a sialic acid binding immunoglobulin-superfamily receptor that is highly expressed on eosinophils. We have used a mouse model of oral egg ovalbumin (OVA)-induced eosinophilic inflammation of the gastro-intestinal mucosa associated with diarrhea and weight loss to determine whether administering an anti-Siglec-F antibody would reduce levels of intestinal mucosal eosinophilic inflammation. Mice administered the anti-Siglec-F antibody had significantly lower levels of intestinal eosinophilic inflammation, and this was associated with reduced intestinal permeability changes, normalization of intestinal villous crypt height, and restoration of weight gain. The reduced numbers of intestinal eosinophils in anti-Siglec-F antibody treated mice was associated with significantly reduced numbers of bone marrow and peripheral blood eosinophils, but was not associated with significant changes in the numbers of proliferating or apoptotic jejunal eosinophils. In addition, the anti-Siglec-F Ab reduced Th2 cytokines and IgE levels. Overall, these studies demonstrate that administration of an anti-Siglec-F antibody significantly reduces levels of eosinophilic inflammation in the intestinal mucosa and that this was associated with reduced intestinal permeability changes, normalization of intestinal villous crypt height, and restoration of weight gain.

    Original languageEnglish
    Pages (from-to)157-169
    Number of pages13
    JournalClinical Immunology
    Volume131
    Issue number1
    DOIs
    Publication statusPublished - 2009 Apr

    Keywords

    • Eosinophils
    • Food allergy
    • Inflammation
    • Siglec-F

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

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