Anti-tumor immunostimulatory effect of heat-killed tumor cells

Joon Yoon Taek, Yeon Kim Ji, Hyojeong Kim, Changwan Hong, Hyunji Lee, Chang Kwon Lee, Ho Lee Kwang, Seokmann Hong, Se-Ho Park

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

As a part of our ongoing search for a safe and efficient anti-tumor vaccine, we attempted to determine whether the molecular nature of certain tumor antigens would influence immune responses against tumor cells. As compared with freeze-thawed or form-aldehyde-fixed tumor antigens, heat-denatured tumor antigens elicited profound anti-tumor immune responses and greatly inhibited the growth of live tumor cells. The heat-denatured tumor antigens induced a substantial increase in the anti-tumor CTL response in the absence of any adjuvant material. This response appears to be initiated by strong activation of the antigen-presenting cells, which may recognize heat-denatured protein antigens. Upon recognition of the heat-denatured tumor antigens, macrophages and dendritic cells were found to acutely upregulate the expression of co-stimulatory molecules such as B7.2, as well as the secretion of inflammatory cytokines such as IL-12 and TNF-α. The results of this study indicate that heat-denatured tumor extracts might elicit protective anti-tumor adaptive immune responses and also raise the possibility that a safe and efficient adjuvant-free tumor vaccine might be developed in conjunction with a dendritic cell-based tumor vaccine.

Original languageEnglish
Pages (from-to)130-144
Number of pages15
JournalExperimental and Molecular Medicine
Volume40
Issue number1
DOIs
Publication statusPublished - 2008 Feb 29

Fingerprint

Neoplasm Antigens
Tumors
Hot Temperature
Cells
Cancer Vaccines
Neoplasms
Dendritic Cells
Macrophages
Adaptive Immunity
Antigen-Presenting Cells
Interleukin-12
Aldehydes
Up-Regulation
Chemical activation
Cytokines
Antigens
Molecules
Growth
Proteins

Keywords

  • Cancer vaccines
  • Dendritic cells
  • Immunotherapy, active
  • Interleukin 12
  • Mice

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Anti-tumor immunostimulatory effect of heat-killed tumor cells. / Taek, Joon Yoon; Ji, Yeon Kim; Kim, Hyojeong; Hong, Changwan; Lee, Hyunji; Lee, Chang Kwon; Kwang, Ho Lee; Hong, Seokmann; Park, Se-Ho.

In: Experimental and Molecular Medicine, Vol. 40, No. 1, 29.02.2008, p. 130-144.

Research output: Contribution to journalArticle

Taek, JY, Ji, YK, Kim, H, Hong, C, Lee, H, Lee, CK, Kwang, HL, Hong, S & Park, S-H 2008, 'Anti-tumor immunostimulatory effect of heat-killed tumor cells', Experimental and Molecular Medicine, vol. 40, no. 1, pp. 130-144. https://doi.org/10.3858/emm.2008.40.1.130
Taek, Joon Yoon ; Ji, Yeon Kim ; Kim, Hyojeong ; Hong, Changwan ; Lee, Hyunji ; Lee, Chang Kwon ; Kwang, Ho Lee ; Hong, Seokmann ; Park, Se-Ho. / Anti-tumor immunostimulatory effect of heat-killed tumor cells. In: Experimental and Molecular Medicine. 2008 ; Vol. 40, No. 1. pp. 130-144.
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