Anticancer activity of lactoferrin isolated from caprine colostrum on human cancer cell lines

Younghoon Kim, Mae Jin Kim, Kyoung Sik Han, Jee Young Imm, Sejong Oh, Sae Hun Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In the present study, lactoferrin was purified from caprine colostrum and its anticancer effects were investigated on various cancer cell lines, including lung, colon, cervix, stomach and breast cancer cells. The caprine lactoferrin (CLF) was purified by ultrafiltration and sequential chromatography using a CM-Sephadex C-50-120 ion-exchange column and affinity chromatography on Hi-TrapTM Heparin HP. The purified CLF exhibited antiproliferative effects on the five tested cancer cell lines in a dose-dependent manner, with 20-30% survival as compared to the control. Interestingly, the most intense inhibition of cell growth was observed on the ZR-75-1 breast cancer cells (IC50 = 27.5 μg/mL). Our findings suggest, for the first time, that purified CLF from caprine colostrum may constitute a novel anticancer agent for the food industry.

Original languageEnglish
Pages (from-to)277-281
Number of pages5
JournalInternational Journal of Dairy Technology
Volume62
Issue number2
DOIs
Publication statusPublished - 2009 May 1

Keywords

  • Anticancer activity
  • Caprine lactoferrin
  • Chromatography
  • Human cancer cell lines
  • Purification

ASJC Scopus subject areas

  • Food Science
  • Bioengineering
  • Process Chemistry and Technology

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