Antidepressant effect of stem cell-derived monoaminergic grafts

Miles G. Cunningham, Rachael A. Donalds, William A. Carlezon, Sunghoi Hong, Dae-Sung Kim, Dong Wook Kim, Kwang Soo Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this study, we demonstrate that embryonic stem cells can be engineered to differentiate into high percentages of serotonergic and dopaminergic neurons. In vitro, these cells release serotonin and dopamine in response to membrane depolarization. Upon engraftment into the medial prefrontal cortex in rats, the homolog of the human anterior cingulate cortex, the cells assumed neuronal morphologies, expressed monoaminergic-specific proteins, and seemed to functionally integrate, as assessed by the upregulation of the immediate-early gene, cfos. Furthermore, the transplanted animals performed in a manner similar to that of animals that received the antidepressant, citalopram, when administered the forced swim test, a validated model of human depression. These results suggest that transplantation of customized stem cells might perhaps be useful in the study treatment of psychiatric disorders.

Original languageEnglish
Pages (from-to)1663-1667
Number of pages5
JournalNeuroReport
Volume18
Issue number16
DOIs
Publication statusPublished - 2007 Oct 1
Externally publishedYes

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Keywords

  • Depression
  • Dopamine
  • Embryonic stemcells
  • Forced swim test
  • Immunofluorescence
  • Neural transplantation
  • Serotonin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cunningham, M. G., Donalds, R. A., Carlezon, W. A., Hong, S., Kim, D-S., Kim, D. W., & Kim, K. S. (2007). Antidepressant effect of stem cell-derived monoaminergic grafts. NeuroReport, 18(16), 1663-1667. https://doi.org/10.1097/WNR.0b013e3282f0eb1c