Antifouling membranes employing a 2D planar nanobiocatalyst of crosslinked glucose oxidase aggregates wrapping extra-large graphene oxide

Testaverde S. Kim, Jahyun Nam, Dae Woo Kim, Hee Tae Jung, Kyung Min Yeon, Jungbae Kim

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

This paper presents highly effective antimicrobial surfaces employing a 2D structured nanobiocatalyst composed of graphene oxide (GO) and glucose oxidase (GOD). Enzyme molecules are immobilized onto extra-large GO pieces with a plane dimension of approximately 100 μm via an enzyme adsorption, precipitation, and crosslinking (EAPC) approach. This enables the effective wrapping of extra-large GO pieces by a matrix of crosslinked enzyme aggregates, which improves the enzyme loading. Consequently, the measured GOD activities of the EAPC sample via 50% (w/v) ammonium sulfate precipitation are 4,940 and 3,820 times higher than those of the control samples, i.e, the enzyme adsorption (EA) and enzyme adsorption/crosslinking (EAC) samples, respectively. The preservation of the planar GO geometry with an extra-large surface also allows the effective binding of EAPC onto a commercial membrane filter via a polydopamine coating, thus yielding a biocatalytic EAPC membrane. Compared to the commercial membrane with no bound EAPC, the in situ generation of H2O2 via the EAPC-catalyzed oxidation of glucose on the membrane surface demonstrated enhanced filterability against a mixed bacterial population of activated sludge obtained from a municipal sewage plant as well as two model bacteria: gram-negative Pseudomonas aeruginosa and gram-positive Staphylococcus aureus. The bacterial decontamination of the EAPC-bound membrane surface can also be activated on demand by simply adding glucose to the bulk solution. This newly proposed mechanism of antifouling surfaces employing a localized nanobiocatalytic conversion of nontoxic glucose to bactericidal H2O2 can provide insights for biofouling control via a highly effective and environment-friendly approach.

Original languageEnglish
Article number130343
JournalChemical Engineering Journal
Volume424
DOIs
Publication statusPublished - 2021 Nov 15

Keywords

  • Antimicrobial surface
  • Glucose oxidase
  • Graphene oxide
  • In situ biocide generation
  • Membrane antifouling
  • Planar nanobiocatalyst

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

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