Antigenicity of the region encoded by exon8 of the human serine protease, HtrA2/Omi, is associated with its protein solubility

Hyo Jin Park, Ju Youn Choi, Young Mo Seong, Chul Han, Sung Joo Kim Yoon, Seong Man Kang, Hyangshuk Rhim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

HtrA2/Omi, a mitochondrial serine protease, is pivotal in regulating apoptotic cell death. To determine the location of antigenic determinants in HtrA2/Omi, we expressed a series of the N-terminally truncated HtrA2/Omi as GST fusion proteins in E. coli. We assessed protein solubility and antigenic reactivity of various N-terminally truncated HtrA2/Omi proteins by binding to glutathione beads and immunoblot analyses, respectively. We identified that the region encoded by exon8 of HtrA2/Omi was expressed as a highly soluble form and contains an antigenic determinant specifically recognized by a polyclonal serum against HtrA2/Omi. Our data provide evidence that protein solubility of the specific region in target proteins may contribute to the antigenicity.

Original languageEnglish
Pages (from-to)1597-1603
Number of pages7
JournalBiotechnology Letters
Volume25
Issue number19
DOIs
Publication statusPublished - 2003 Oct 1

Fingerprint

Serine Proteases
Solubility
Proteins
Epitopes
Escherichia coli Proteins
Protein Binding
Glutathione
Cell Death
Cell death
Escherichia coli
Serum
Fusion reactions
Omi serine protease
Peptide Hydrolases

Keywords

  • Antigenic determinant
  • Exon8
  • pGEX expression
  • Protein solubility

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Antigenicity of the region encoded by exon8 of the human serine protease, HtrA2/Omi, is associated with its protein solubility. / Park, Hyo Jin; Choi, Ju Youn; Seong, Young Mo; Han, Chul; Yoon, Sung Joo Kim; Kang, Seong Man; Rhim, Hyangshuk.

In: Biotechnology Letters, Vol. 25, No. 19, 01.10.2003, p. 1597-1603.

Research output: Contribution to journalArticle

Park, Hyo Jin ; Choi, Ju Youn ; Seong, Young Mo ; Han, Chul ; Yoon, Sung Joo Kim ; Kang, Seong Man ; Rhim, Hyangshuk. / Antigenicity of the region encoded by exon8 of the human serine protease, HtrA2/Omi, is associated with its protein solubility. In: Biotechnology Letters. 2003 ; Vol. 25, No. 19. pp. 1597-1603.
@article{1b31397e1ec443b7b682733a7d267bbe,
title = "Antigenicity of the region encoded by exon8 of the human serine protease, HtrA2/Omi, is associated with its protein solubility",
abstract = "HtrA2/Omi, a mitochondrial serine protease, is pivotal in regulating apoptotic cell death. To determine the location of antigenic determinants in HtrA2/Omi, we expressed a series of the N-terminally truncated HtrA2/Omi as GST fusion proteins in E. coli. We assessed protein solubility and antigenic reactivity of various N-terminally truncated HtrA2/Omi proteins by binding to glutathione beads and immunoblot analyses, respectively. We identified that the region encoded by exon8 of HtrA2/Omi was expressed as a highly soluble form and contains an antigenic determinant specifically recognized by a polyclonal serum against HtrA2/Omi. Our data provide evidence that protein solubility of the specific region in target proteins may contribute to the antigenicity.",
keywords = "Antigenic determinant, Exon8, pGEX expression, Protein solubility",
author = "Park, {Hyo Jin} and Choi, {Ju Youn} and Seong, {Young Mo} and Chul Han and Yoon, {Sung Joo Kim} and Kang, {Seong Man} and Hyangshuk Rhim",
year = "2003",
month = "10",
day = "1",
doi = "10.1023/A:1025630013679",
language = "English",
volume = "25",
pages = "1597--1603",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "19",

}

TY - JOUR

T1 - Antigenicity of the region encoded by exon8 of the human serine protease, HtrA2/Omi, is associated with its protein solubility

AU - Park, Hyo Jin

AU - Choi, Ju Youn

AU - Seong, Young Mo

AU - Han, Chul

AU - Yoon, Sung Joo Kim

AU - Kang, Seong Man

AU - Rhim, Hyangshuk

PY - 2003/10/1

Y1 - 2003/10/1

N2 - HtrA2/Omi, a mitochondrial serine protease, is pivotal in regulating apoptotic cell death. To determine the location of antigenic determinants in HtrA2/Omi, we expressed a series of the N-terminally truncated HtrA2/Omi as GST fusion proteins in E. coli. We assessed protein solubility and antigenic reactivity of various N-terminally truncated HtrA2/Omi proteins by binding to glutathione beads and immunoblot analyses, respectively. We identified that the region encoded by exon8 of HtrA2/Omi was expressed as a highly soluble form and contains an antigenic determinant specifically recognized by a polyclonal serum against HtrA2/Omi. Our data provide evidence that protein solubility of the specific region in target proteins may contribute to the antigenicity.

AB - HtrA2/Omi, a mitochondrial serine protease, is pivotal in regulating apoptotic cell death. To determine the location of antigenic determinants in HtrA2/Omi, we expressed a series of the N-terminally truncated HtrA2/Omi as GST fusion proteins in E. coli. We assessed protein solubility and antigenic reactivity of various N-terminally truncated HtrA2/Omi proteins by binding to glutathione beads and immunoblot analyses, respectively. We identified that the region encoded by exon8 of HtrA2/Omi was expressed as a highly soluble form and contains an antigenic determinant specifically recognized by a polyclonal serum against HtrA2/Omi. Our data provide evidence that protein solubility of the specific region in target proteins may contribute to the antigenicity.

KW - Antigenic determinant

KW - Exon8

KW - pGEX expression

KW - Protein solubility

UR - http://www.scopus.com/inward/record.url?scp=0141638591&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141638591&partnerID=8YFLogxK

U2 - 10.1023/A:1025630013679

DO - 10.1023/A:1025630013679

M3 - Article

C2 - 14584913

AN - SCOPUS:0141638591

VL - 25

SP - 1597

EP - 1603

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 19

ER -