Antigenicity of the region encoded by exon8 of the human serine protease, HtrA2/Omi, is associated with its protein solubility

Hyo Jin Park, Ju Youn Choi, Young Mo Seong, Chul Han, Sung Joo Kim Yoon, Seongman Kang, Hyangshuk Rhim

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

HtrA2/Omi, a mitochondrial serine protease, is pivotal in regulating apoptotic cell death. To determine the location of antigenic determinants in HtrA2/Omi, we expressed a series of the N-terminally truncated HtrA2/Omi as GST fusion proteins in E. coli. We assessed protein solubility and antigenic reactivity of various N-terminally truncated HtrA2/Omi proteins by binding to glutathione beads and immunoblot analyses, respectively. We identified that the region encoded by exon8 of HtrA2/Omi was expressed as a highly soluble form and contains an antigenic determinant specifically recognized by a polyclonal serum against HtrA2/Omi. Our data provide evidence that protein solubility of the specific region in target proteins may contribute to the antigenicity.

Original languageEnglish
Pages (from-to)1597-1603
Number of pages7
JournalBiotechnology letters
Volume25
Issue number19
DOIs
Publication statusPublished - 2003 Oct

Keywords

  • Antigenic determinant
  • Exon8
  • Protein solubility
  • pGEX expression

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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