Antimicrobial activity of NO-releasing compounds against periodontal pathogens

Ji Suk Shim, Dong Sik Park, Dong Heon Baek, Nayansi Jha, Serkin Park, Hyoung Jin Yun, Won Jong Kim, Jae Jun Ryu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study describes the successful synthesis of nitric oxide (NO)-releasing compounds with biodegradable and injectable properties and demonstrates that the kinetics of NO release vary according to the type of NO donor. The antimicrobial activity of NO-releasing compounds against three common periodontal pathogens, i.e., Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Actinomyces israelii, was investigated using a susceptibility assay. Human gingival fibroblasts were treated with NO-releasing compounds at the minimum concentrations required for bacterial growth and cytotoxicity was evaluated using the MTT cell proliferation assay. Our results suggest that NO-releasing compounds can be used topically to treat both gram-negative and gram-positive periodontal pathogens. Comparison of the antimicrobial activity and cytotoxicity assay results between the NO-releasing compounds revealed that an NO donor comprising a macromolecule without surface charge, a lower instantaneous NO concentration, and an adequate supply of NO were associated with a strong bactericidal effect and low cytotoxicity. NO-releasing compounds with these properties may be suitable for treatment of periodontitis.

Original languageEnglish
Article numbere0199998
JournalPLoS One
Volume13
Issue number10
DOIs
Publication statusPublished - 2018 Oct 1

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Pathogens
nitric oxide
Nitric Oxide
anti-infective agents
pathogens
Cytotoxicity
Assays
Nitric Oxide Donors
cytotoxicity
Actinomyces israelii
Actinobacillus actinomycetemcomitans
Aggregatibacter actinomycetemcomitans
assays
Actinomyces
Porphyromonas gingivalis
Periodontitis
Cell proliferation
Fibroblasts
Surface charge
biodegradability

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Antimicrobial activity of NO-releasing compounds against periodontal pathogens. / Shim, Ji Suk; Park, Dong Sik; Baek, Dong Heon; Jha, Nayansi; Park, Serkin; Yun, Hyoung Jin; Kim, Won Jong; Ryu, Jae Jun.

In: PLoS One, Vol. 13, No. 10, e0199998, 01.10.2018.

Research output: Contribution to journalArticle

Shim, Ji Suk ; Park, Dong Sik ; Baek, Dong Heon ; Jha, Nayansi ; Park, Serkin ; Yun, Hyoung Jin ; Kim, Won Jong ; Ryu, Jae Jun. / Antimicrobial activity of NO-releasing compounds against periodontal pathogens. In: PLoS One. 2018 ; Vol. 13, No. 10.
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