Antimicrobial activity of NO-releasing compounds against periodontal pathogens

Ji Suk Shim, Dong Sik Park, Dong Heon Baek, Nayansi Jha, Serkin Park, Hyoung Jin Yun, Won Jong Kim, Jae Jun Ryu

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3 Citations (Scopus)


This study describes the successful synthesis of nitric oxide (NO)-releasing compounds with biodegradable and injectable properties and demonstrates that the kinetics of NO release vary according to the type of NO donor. The antimicrobial activity of NO-releasing compounds against three common periodontal pathogens, i.e., Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Actinomyces israelii, was investigated using a susceptibility assay. Human gingival fibroblasts were treated with NO-releasing compounds at the minimum concentrations required for bacterial growth and cytotoxicity was evaluated using the MTT cell proliferation assay. Our results suggest that NO-releasing compounds can be used topically to treat both gram-negative and gram-positive periodontal pathogens. Comparison of the antimicrobial activity and cytotoxicity assay results between the NO-releasing compounds revealed that an NO donor comprising a macromolecule without surface charge, a lower instantaneous NO concentration, and an adequate supply of NO were associated with a strong bactericidal effect and low cytotoxicity. NO-releasing compounds with these properties may be suitable for treatment of periodontitis.

Original languageEnglish
Article numbere0199998
JournalPLoS One
Issue number10
Publication statusPublished - 2018 Oct 1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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  • Cite this

    Shim, J. S., Park, D. S., Baek, D. H., Jha, N., Park, S., Yun, H. J., Kim, W. J., & Ryu, J. J. (2018). Antimicrobial activity of NO-releasing compounds against periodontal pathogens. PLoS One, 13(10), [e0199998].