Abstract
Defensins, a prominent group of antimicrobial peptides, are an important component of the innate immune response, particularly at mucosal surfaces that are vulnerable to colonization by potential pathogens. The present study was undertaken to investigate the expression of defensins in inferior turbinate mucosa of normal subjects and inferior turbinate mucosa and nasal polyps of patients with chronic sinusitis. Expression of β-defensin 1 and 2 and α-defensin 5 and 6 messenger RNAs (mRNAs) was investigated by reverse transcriptase-polymerase chain reaction, and their expression level was semiquantitatively evaluated by dot blot hybridization. Immunohistochemical analysis was used for detection of α-defensins 1, 2, and 3 in tissue sections. β-Defensin 1 mRNA was expressed in all tissue samples, at levels that did not differ significantly. β-Defensin 2 mRNA was detected in the turbinate mucosa and nasal polyps of patients with chronic sinusitis, but not in normal mucosa. Its expression level was significantly higher in nasal polyps than in turbinate mucosa. α-Defensin 5 and 6 mRNAs were not expressed in any tissues, but α-defensins 1, 2, and 3 were detected in all tissue samples obtained from patients with chronic sinusitis. These results suggest that β-defensin 1 may play a constitutive role in nasal defenses, whereas α-defensins 1, 2, and 3 and β-defensin 2 may be induced in response to local infection or inflammation.
| Original language | English |
|---|---|
| Pages (from-to) | 135-141 |
| Number of pages | 7 |
| Journal | Annals of Otology, Rhinology and Laryngology |
| Volume | 111 |
| Issue number | 2 |
| Publication status | Published - 2002 Feb 18 |
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Keywords
- Defensin
- Inferior turbinate
- Nasal polyp
ASJC Scopus subject areas
- Otorhinolaryngology
Cite this
Antimicrobial defensin peptides of the human nasal mucosa. / Lee, Sang Hag; Jung, Eun Kim; Hyun, Ho Lim; Lee, Heung Man; Jong, Ouck Choi.
In: Annals of Otology, Rhinology and Laryngology, Vol. 111, No. 2, 18.02.2002, p. 135-141.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antimicrobial defensin peptides of the human nasal mucosa
AU - Lee, Sang Hag
AU - Jung, Eun Kim
AU - Hyun, Ho Lim
AU - Lee, Heung Man
AU - Jong, Ouck Choi
PY - 2002/2/18
Y1 - 2002/2/18
N2 - Defensins, a prominent group of antimicrobial peptides, are an important component of the innate immune response, particularly at mucosal surfaces that are vulnerable to colonization by potential pathogens. The present study was undertaken to investigate the expression of defensins in inferior turbinate mucosa of normal subjects and inferior turbinate mucosa and nasal polyps of patients with chronic sinusitis. Expression of β-defensin 1 and 2 and α-defensin 5 and 6 messenger RNAs (mRNAs) was investigated by reverse transcriptase-polymerase chain reaction, and their expression level was semiquantitatively evaluated by dot blot hybridization. Immunohistochemical analysis was used for detection of α-defensins 1, 2, and 3 in tissue sections. β-Defensin 1 mRNA was expressed in all tissue samples, at levels that did not differ significantly. β-Defensin 2 mRNA was detected in the turbinate mucosa and nasal polyps of patients with chronic sinusitis, but not in normal mucosa. Its expression level was significantly higher in nasal polyps than in turbinate mucosa. α-Defensin 5 and 6 mRNAs were not expressed in any tissues, but α-defensins 1, 2, and 3 were detected in all tissue samples obtained from patients with chronic sinusitis. These results suggest that β-defensin 1 may play a constitutive role in nasal defenses, whereas α-defensins 1, 2, and 3 and β-defensin 2 may be induced in response to local infection or inflammation.
AB - Defensins, a prominent group of antimicrobial peptides, are an important component of the innate immune response, particularly at mucosal surfaces that are vulnerable to colonization by potential pathogens. The present study was undertaken to investigate the expression of defensins in inferior turbinate mucosa of normal subjects and inferior turbinate mucosa and nasal polyps of patients with chronic sinusitis. Expression of β-defensin 1 and 2 and α-defensin 5 and 6 messenger RNAs (mRNAs) was investigated by reverse transcriptase-polymerase chain reaction, and their expression level was semiquantitatively evaluated by dot blot hybridization. Immunohistochemical analysis was used for detection of α-defensins 1, 2, and 3 in tissue sections. β-Defensin 1 mRNA was expressed in all tissue samples, at levels that did not differ significantly. β-Defensin 2 mRNA was detected in the turbinate mucosa and nasal polyps of patients with chronic sinusitis, but not in normal mucosa. Its expression level was significantly higher in nasal polyps than in turbinate mucosa. α-Defensin 5 and 6 mRNAs were not expressed in any tissues, but α-defensins 1, 2, and 3 were detected in all tissue samples obtained from patients with chronic sinusitis. These results suggest that β-defensin 1 may play a constitutive role in nasal defenses, whereas α-defensins 1, 2, and 3 and β-defensin 2 may be induced in response to local infection or inflammation.
KW - Defensin
KW - Inferior turbinate
KW - Nasal polyp
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M3 - Article
C2 - 11860065
AN - SCOPUS:0036162234
VL - 111
SP - 135
EP - 141
JO - Annals of Otology, Rhinology and Laryngology
JF - Annals of Otology, Rhinology and Laryngology
SN - 0003-4894
IS - 2
ER -