Antiobesity effect of PEGylated conjugated linoleic acid on high-fat diet-induced obese C57BL/6J (ob/ob) mice: attenuation of insulin resistance and enhancement of antioxidant defenses

Hyun-Seuk Moon, Hong Gu Lee, Ji Hye Seo, Chung Soo Chung, Tae Gyu Kim, Yun Jaie Choi, Chong Su Cho

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

This study was designed to test that dietary conjugated linoleic acids (CLA) used in a mixture of cis-9,trans-11 and trans-10, cis-12 isomers (40% each in weight) coupled to poly(ethylene glycol) (PEG) as PEGylated CLA (PCLA) act as mediators inducing or inhibiting specific metabolic pathways in high-fat (HF)-fed obese C57BL/6J (ob/ob) mice. After an acclimatization period of 7 days, animals were given a normal (control) or HF diet, the latter being added either alone (HF) or with CLA, PEG or PCLA for 6 weeks. Although the food intakes were not different among the dietary groups, final body weights were significantly lower in the HF-PCLA group than in the HF group. Also the HF-PCLA diet strongly prevented the dramatic increase in blood low-density lipoprotein cholesterol observed with the HF diet, with no difference in high-density lipoprotein cholesterol between control, HF and HF-PCLA treatments. Furthermore, homeostasis model assessment levels showed a marked decrease in HF-PCLA-fed mice, preventing the increase found in mice fed the HF diet, and suggesting that PCLA lowered insulin resistance in HF-mice. The liver steatosis observed in mice fed the HF diet was also prevented by PCLA. Interestingly, the activity of mitochondrial glutathione peroxidase was increased by PCLA, which may enhance antioxidant defenses. Overall, PCLA exerted its beneficial effects through reduction of lipid accumulation and attenuation of insulin resistance induced by the HF diet in obese C57BL/6J (ob/ob) mice, which might confer to these products antiobesity properties in other species.

Original languageEnglish
Pages (from-to)187-194
Number of pages8
JournalJournal of Nutritional Biochemistry
Volume20
Issue number3
DOIs
Publication statusPublished - 2009 Mar 1
Externally publishedYes

Fingerprint

Conjugated Linoleic Acids
High Fat Diet
Nutrition
Insulin Resistance
Antioxidants
Fats
Insulin
Polyethylene glycols
Ethylene Glycol
Acclimatization
Fatty Liver
Glutathione Peroxidase
Metabolic Networks and Pathways
LDL Cholesterol
HDL Cholesterol
Homeostasis
Eating
Body Weight
Diet
Lipids

Keywords

  • Anti-oxidant defense
  • C57BL/6J (ob/ob) mouse
  • Insulin resistance
  • Lipid reduction
  • PEGylated conjugated linoleic acid

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Antiobesity effect of PEGylated conjugated linoleic acid on high-fat diet-induced obese C57BL/6J (ob/ob) mice : attenuation of insulin resistance and enhancement of antioxidant defenses. / Moon, Hyun-Seuk; Lee, Hong Gu; Seo, Ji Hye; Chung, Chung Soo; Kim, Tae Gyu; Choi, Yun Jaie; Cho, Chong Su.

In: Journal of Nutritional Biochemistry, Vol. 20, No. 3, 01.03.2009, p. 187-194.

Research output: Contribution to journalArticle

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abstract = "This study was designed to test that dietary conjugated linoleic acids (CLA) used in a mixture of cis-9,trans-11 and trans-10, cis-12 isomers (40{\%} each in weight) coupled to poly(ethylene glycol) (PEG) as PEGylated CLA (PCLA) act as mediators inducing or inhibiting specific metabolic pathways in high-fat (HF)-fed obese C57BL/6J (ob/ob) mice. After an acclimatization period of 7 days, animals were given a normal (control) or HF diet, the latter being added either alone (HF) or with CLA, PEG or PCLA for 6 weeks. Although the food intakes were not different among the dietary groups, final body weights were significantly lower in the HF-PCLA group than in the HF group. Also the HF-PCLA diet strongly prevented the dramatic increase in blood low-density lipoprotein cholesterol observed with the HF diet, with no difference in high-density lipoprotein cholesterol between control, HF and HF-PCLA treatments. Furthermore, homeostasis model assessment levels showed a marked decrease in HF-PCLA-fed mice, preventing the increase found in mice fed the HF diet, and suggesting that PCLA lowered insulin resistance in HF-mice. The liver steatosis observed in mice fed the HF diet was also prevented by PCLA. Interestingly, the activity of mitochondrial glutathione peroxidase was increased by PCLA, which may enhance antioxidant defenses. Overall, PCLA exerted its beneficial effects through reduction of lipid accumulation and attenuation of insulin resistance induced by the HF diet in obese C57BL/6J (ob/ob) mice, which might confer to these products antiobesity properties in other species.",
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