Antioxidant activities and melanogenesis inhibitory effects of terminalia chebula in B16/F10 melanoma cells

Hyun Sun Lee, Hye Jin Cho, Kwang Won Lee, Sung Sun Park, Ho Chan Seo, Hyung Joo Suh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To examine the potential of Terminalia chebula as a whitening agent, we measured antioxidant activity using DPPH· , ABTS·+ assays and ferric-reducing antioxidant power (FRAP) assays, and depigmenting activity using B16F10 melanoma cells. The intracellular reactive oxygen species (ROS) level was monitored by H2DCFDA fluorescence labeling, and melanin contents in B16F10 melanoma cells by 960 J/m2 dose of UVA-induced oxidative stress. The radical-scavenging activities of T. chebula extract (TCE) were measured in terms of EC50 values using DPPH·, ABTS·+ assays and FRAP value were 280.0 μg/mL, 42.2 μg/mL and 113.1 μmol FeSO4 7H 2O/g, respectively. We found that ROS and melanin concentrations were reduced by TCE treatments of 25 μg/mL under UVA-induced oxidative stress. Tyrosinase activity and melanin contents in a-melanocyte stimulating hormone (MSH)-induced melanoma cells both decreased dose-dependently in the treatment groups. TCE similarly reduced melanogenesis in B16F10 melanoma cells stimulated by a-MSH as compared to arbutin as a positive control. T. chebula may prove to be a useful therapeutic agent for hyperpigmentation and an effective component in skin whitening andor lightening cosmetics.

Original languageEnglish
Pages (from-to)213-220
Number of pages8
JournalJournal of Food Science and Nutrition
Volume15
Issue number3
DOIs
Publication statusPublished - 2010 Dec 1

Fingerprint

Terminalia chebula
Terminalia
melanogenesis
Experimental Melanomas
melanoma
Melanoma
Melanins
Antioxidants
antioxidant activity
Melanocyte-Stimulating Hormones
melanin
melanocyte-stimulating hormone
Reactive Oxygen Species
Oxidative Stress
Arbutin
Bleaching Agents
cells
reactive oxygen species
extracts
assays

Keywords

  • B16F10 melanoma cell
  • Melanogenesis
  • Skin whitening
  • Terminalia chebula
  • Ultraviolet

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Antioxidant activities and melanogenesis inhibitory effects of terminalia chebula in B16/F10 melanoma cells. / Lee, Hyun Sun; Cho, Hye Jin; Lee, Kwang Won; Park, Sung Sun; Seo, Ho Chan; Suh, Hyung Joo.

In: Journal of Food Science and Nutrition, Vol. 15, No. 3, 01.12.2010, p. 213-220.

Research output: Contribution to journalArticle

@article{34d2f67793f2483c94210d91af7e6752,
title = "Antioxidant activities and melanogenesis inhibitory effects of terminalia chebula in B16/F10 melanoma cells",
abstract = "To examine the potential of Terminalia chebula as a whitening agent, we measured antioxidant activity using DPPH· , ABTS·+ assays and ferric-reducing antioxidant power (FRAP) assays, and depigmenting activity using B16F10 melanoma cells. The intracellular reactive oxygen species (ROS) level was monitored by H2DCFDA fluorescence labeling, and melanin contents in B16F10 melanoma cells by 960 J/m2 dose of UVA-induced oxidative stress. The radical-scavenging activities of T. chebula extract (TCE) were measured in terms of EC50 values using DPPH·, ABTS·+ assays and FRAP value were 280.0 μg/mL, 42.2 μg/mL and 113.1 μmol FeSO4 7H 2O/g, respectively. We found that ROS and melanin concentrations were reduced by TCE treatments of 25 μg/mL under UVA-induced oxidative stress. Tyrosinase activity and melanin contents in a-melanocyte stimulating hormone (MSH)-induced melanoma cells both decreased dose-dependently in the treatment groups. TCE similarly reduced melanogenesis in B16F10 melanoma cells stimulated by a-MSH as compared to arbutin as a positive control. T. chebula may prove to be a useful therapeutic agent for hyperpigmentation and an effective component in skin whitening andor lightening cosmetics.",
keywords = "B16F10 melanoma cell, Melanogenesis, Skin whitening, Terminalia chebula, Ultraviolet",
author = "Lee, {Hyun Sun} and Cho, {Hye Jin} and Lee, {Kwang Won} and Park, {Sung Sun} and Seo, {Ho Chan} and Suh, {Hyung Joo}",
year = "2010",
month = "12",
day = "1",
doi = "10.3746/jfn.2010.15.3.213",
language = "English",
volume = "15",
pages = "213--220",
journal = "Preventive Nutrition and Food Science",
issn = "2287-1098",
publisher = "Korean Society of Food Science and Nutrition",
number = "3",

}

TY - JOUR

T1 - Antioxidant activities and melanogenesis inhibitory effects of terminalia chebula in B16/F10 melanoma cells

AU - Lee, Hyun Sun

AU - Cho, Hye Jin

AU - Lee, Kwang Won

AU - Park, Sung Sun

AU - Seo, Ho Chan

AU - Suh, Hyung Joo

PY - 2010/12/1

Y1 - 2010/12/1

N2 - To examine the potential of Terminalia chebula as a whitening agent, we measured antioxidant activity using DPPH· , ABTS·+ assays and ferric-reducing antioxidant power (FRAP) assays, and depigmenting activity using B16F10 melanoma cells. The intracellular reactive oxygen species (ROS) level was monitored by H2DCFDA fluorescence labeling, and melanin contents in B16F10 melanoma cells by 960 J/m2 dose of UVA-induced oxidative stress. The radical-scavenging activities of T. chebula extract (TCE) were measured in terms of EC50 values using DPPH·, ABTS·+ assays and FRAP value were 280.0 μg/mL, 42.2 μg/mL and 113.1 μmol FeSO4 7H 2O/g, respectively. We found that ROS and melanin concentrations were reduced by TCE treatments of 25 μg/mL under UVA-induced oxidative stress. Tyrosinase activity and melanin contents in a-melanocyte stimulating hormone (MSH)-induced melanoma cells both decreased dose-dependently in the treatment groups. TCE similarly reduced melanogenesis in B16F10 melanoma cells stimulated by a-MSH as compared to arbutin as a positive control. T. chebula may prove to be a useful therapeutic agent for hyperpigmentation and an effective component in skin whitening andor lightening cosmetics.

AB - To examine the potential of Terminalia chebula as a whitening agent, we measured antioxidant activity using DPPH· , ABTS·+ assays and ferric-reducing antioxidant power (FRAP) assays, and depigmenting activity using B16F10 melanoma cells. The intracellular reactive oxygen species (ROS) level was monitored by H2DCFDA fluorescence labeling, and melanin contents in B16F10 melanoma cells by 960 J/m2 dose of UVA-induced oxidative stress. The radical-scavenging activities of T. chebula extract (TCE) were measured in terms of EC50 values using DPPH·, ABTS·+ assays and FRAP value were 280.0 μg/mL, 42.2 μg/mL and 113.1 μmol FeSO4 7H 2O/g, respectively. We found that ROS and melanin concentrations were reduced by TCE treatments of 25 μg/mL under UVA-induced oxidative stress. Tyrosinase activity and melanin contents in a-melanocyte stimulating hormone (MSH)-induced melanoma cells both decreased dose-dependently in the treatment groups. TCE similarly reduced melanogenesis in B16F10 melanoma cells stimulated by a-MSH as compared to arbutin as a positive control. T. chebula may prove to be a useful therapeutic agent for hyperpigmentation and an effective component in skin whitening andor lightening cosmetics.

KW - B16F10 melanoma cell

KW - Melanogenesis

KW - Skin whitening

KW - Terminalia chebula

KW - Ultraviolet

UR - http://www.scopus.com/inward/record.url?scp=84866492801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866492801&partnerID=8YFLogxK

U2 - 10.3746/jfn.2010.15.3.213

DO - 10.3746/jfn.2010.15.3.213

M3 - Article

VL - 15

SP - 213

EP - 220

JO - Preventive Nutrition and Food Science

JF - Preventive Nutrition and Food Science

SN - 2287-1098

IS - 3

ER -