Antioxidant and glycation inhibitory activities of gold kiwifruit, Actinidia chinensis

Yanhouy Lee, Chong Oui Hong, Mi Hyun Nam, Ji Hoon Kim, Yuanyuan Ma, Young Bu Kim, Kwang Won Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Oxidative stress has been postulated to contribute significantly to the accelerated accumulation of advanced glycoxidation endproducts (AGEs) in collagen, which is implicated in the process of skin aging. Effectiveness of Actinidia chinensis, commonly called gold kiwifruit, in counteracting skin aging was investigated. Firstly, primary crude 70% ethanolic extracts of whole A. chinensis, pulp, and rind were screened for their in vitro antioxidant activities and anti-glycation activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP) assay, and bovine serum albumin-derived glycation model. Result indicated that rind portion exhibited significantly (p<0.05) high antioxidant activity as well as high phenolic and flavonoid contents compared to those of pulp and whole A. chinensis. Thus, rind was selected for further fractionated with hexane, chloroform, ethyl acetate, and butanol. Among these solvent fractions, A. chinensis rind ethyl acetate (ACRE-E) had the greatest radical-scavenging activity and reducing power, comparable to standard antioxidant, vitamin C. Immunofluorescence staining was used to determine AGEs distribution in glycated collagen matrix. ACRE-E inhibited formation of 67% AGEs. High Performance Liquid Chromatography analysis revealed phenolic compound of ACRE-E as quercetin-3-rhamnoside. High antioxidant and anti-glycation activities of ACRE-E in glycated collagen model indicate its contribution to anti-aging process. A. chinensis rind, previously considered as a byproduct, may have potential as a low-cost raw material for cosmetic and pharmaceutical industries.

Original languageEnglish
Pages (from-to)460-467
Number of pages8
JournalJournal of Applied Biological Chemistry
Volume54
Issue number3
DOIs
Publication statusPublished - 2011 Jul 25

Fingerprint

Actinidia
Antioxidants
Gold
Collagen
Skin Aging
Aging of materials
Pulp
Skin
Flavonoids
Butanols
Oxidative stress
Cosmetics
Vitamins
Scavenging
Quercetin
Drug Industry
High performance liquid chromatography
Hexanes
Chloroform
Bovine Serum Albumin

Keywords

  • Actinidia chinensis
  • Anti-glycation
  • Antioxidant
  • Collagen
  • Gold kiwi
  • Quercetrin

ASJC Scopus subject areas

  • Organic Chemistry
  • Bioengineering
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Antioxidant and glycation inhibitory activities of gold kiwifruit, Actinidia chinensis. / Lee, Yanhouy; Hong, Chong Oui; Nam, Mi Hyun; Kim, Ji Hoon; Ma, Yuanyuan; Kim, Young Bu; Lee, Kwang Won.

In: Journal of Applied Biological Chemistry, Vol. 54, No. 3, 25.07.2011, p. 460-467.

Research output: Contribution to journalArticle

Lee, Yanhouy ; Hong, Chong Oui ; Nam, Mi Hyun ; Kim, Ji Hoon ; Ma, Yuanyuan ; Kim, Young Bu ; Lee, Kwang Won. / Antioxidant and glycation inhibitory activities of gold kiwifruit, Actinidia chinensis. In: Journal of Applied Biological Chemistry. 2011 ; Vol. 54, No. 3. pp. 460-467.
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abstract = "Oxidative stress has been postulated to contribute significantly to the accelerated accumulation of advanced glycoxidation endproducts (AGEs) in collagen, which is implicated in the process of skin aging. Effectiveness of Actinidia chinensis, commonly called gold kiwifruit, in counteracting skin aging was investigated. Firstly, primary crude 70{\%} ethanolic extracts of whole A. chinensis, pulp, and rind were screened for their in vitro antioxidant activities and anti-glycation activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP) assay, and bovine serum albumin-derived glycation model. Result indicated that rind portion exhibited significantly (p<0.05) high antioxidant activity as well as high phenolic and flavonoid contents compared to those of pulp and whole A. chinensis. Thus, rind was selected for further fractionated with hexane, chloroform, ethyl acetate, and butanol. Among these solvent fractions, A. chinensis rind ethyl acetate (ACRE-E) had the greatest radical-scavenging activity and reducing power, comparable to standard antioxidant, vitamin C. Immunofluorescence staining was used to determine AGEs distribution in glycated collagen matrix. ACRE-E inhibited formation of 67{\%} AGEs. High Performance Liquid Chromatography analysis revealed phenolic compound of ACRE-E as quercetin-3-rhamnoside. High antioxidant and anti-glycation activities of ACRE-E in glycated collagen model indicate its contribution to anti-aging process. A. chinensis rind, previously considered as a byproduct, may have potential as a low-cost raw material for cosmetic and pharmaceutical industries.",
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