Abstract
Oxidative stress has been postulated to contribute significantly to the accelerated accumulation of advanced glycoxidation endproducts (AGEs) in collagen, which is implicated in the process of skin aging. Effectiveness of Actinidia chinensis, commonly called gold kiwifruit, in counteracting skin aging was investigated. Firstly, primary crude 70% ethanolic extracts of whole A. chinensis, pulp, and rind were screened for their in vitro antioxidant activities and anti-glycation activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP) assay, and bovine serum albumin-derived glycation model. Result indicated that rind portion exhibited significantly (p<0.05) high antioxidant activity as well as high phenolic and flavonoid contents compared to those of pulp and whole A. chinensis. Thus, rind was selected for further fractionated with hexane, chloroform, ethyl acetate, and butanol. Among these solvent fractions, A. chinensis rind ethyl acetate (ACRE-E) had the greatest radical-scavenging activity and reducing power, comparable to standard antioxidant, vitamin C. Immunofluorescence staining was used to determine AGEs distribution in glycated collagen matrix. ACRE-E inhibited formation of 67% AGEs. High Performance Liquid Chromatography analysis revealed phenolic compound of ACRE-E as quercetin-3-rhamnoside. High antioxidant and anti-glycation activities of ACRE-E in glycated collagen model indicate its contribution to anti-aging process. A. chinensis rind, previously considered as a byproduct, may have potential as a low-cost raw material for cosmetic and pharmaceutical industries.
| Original language | English |
|---|---|
| Pages (from-to) | 460-467 |
| Number of pages | 8 |
| Journal | Journal of Applied Biological Chemistry |
| Volume | 54 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2011 Jul 25 |
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Keywords
- Actinidia chinensis
- Anti-glycation
- Antioxidant
- Collagen
- Gold kiwi
- Quercetrin
ASJC Scopus subject areas
- Organic Chemistry
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Antioxidant and glycation inhibitory activities of gold kiwifruit, Actinidia chinensis. / Lee, Yanhouy; Hong, Chong Oui; Nam, Mi Hyun; Kim, Ji Hoon; Ma, Yuanyuan; Kim, Young Bu; Lee, Kwang Won.
In: Journal of Applied Biological Chemistry, Vol. 54, No. 3, 25.07.2011, p. 460-467.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antioxidant and glycation inhibitory activities of gold kiwifruit, Actinidia chinensis
AU - Lee, Yanhouy
AU - Hong, Chong Oui
AU - Nam, Mi Hyun
AU - Kim, Ji Hoon
AU - Ma, Yuanyuan
AU - Kim, Young Bu
AU - Lee, Kwang Won
PY - 2011/7/25
Y1 - 2011/7/25
N2 - Oxidative stress has been postulated to contribute significantly to the accelerated accumulation of advanced glycoxidation endproducts (AGEs) in collagen, which is implicated in the process of skin aging. Effectiveness of Actinidia chinensis, commonly called gold kiwifruit, in counteracting skin aging was investigated. Firstly, primary crude 70% ethanolic extracts of whole A. chinensis, pulp, and rind were screened for their in vitro antioxidant activities and anti-glycation activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP) assay, and bovine serum albumin-derived glycation model. Result indicated that rind portion exhibited significantly (p<0.05) high antioxidant activity as well as high phenolic and flavonoid contents compared to those of pulp and whole A. chinensis. Thus, rind was selected for further fractionated with hexane, chloroform, ethyl acetate, and butanol. Among these solvent fractions, A. chinensis rind ethyl acetate (ACRE-E) had the greatest radical-scavenging activity and reducing power, comparable to standard antioxidant, vitamin C. Immunofluorescence staining was used to determine AGEs distribution in glycated collagen matrix. ACRE-E inhibited formation of 67% AGEs. High Performance Liquid Chromatography analysis revealed phenolic compound of ACRE-E as quercetin-3-rhamnoside. High antioxidant and anti-glycation activities of ACRE-E in glycated collagen model indicate its contribution to anti-aging process. A. chinensis rind, previously considered as a byproduct, may have potential as a low-cost raw material for cosmetic and pharmaceutical industries.
AB - Oxidative stress has been postulated to contribute significantly to the accelerated accumulation of advanced glycoxidation endproducts (AGEs) in collagen, which is implicated in the process of skin aging. Effectiveness of Actinidia chinensis, commonly called gold kiwifruit, in counteracting skin aging was investigated. Firstly, primary crude 70% ethanolic extracts of whole A. chinensis, pulp, and rind were screened for their in vitro antioxidant activities and anti-glycation activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP) assay, and bovine serum albumin-derived glycation model. Result indicated that rind portion exhibited significantly (p<0.05) high antioxidant activity as well as high phenolic and flavonoid contents compared to those of pulp and whole A. chinensis. Thus, rind was selected for further fractionated with hexane, chloroform, ethyl acetate, and butanol. Among these solvent fractions, A. chinensis rind ethyl acetate (ACRE-E) had the greatest radical-scavenging activity and reducing power, comparable to standard antioxidant, vitamin C. Immunofluorescence staining was used to determine AGEs distribution in glycated collagen matrix. ACRE-E inhibited formation of 67% AGEs. High Performance Liquid Chromatography analysis revealed phenolic compound of ACRE-E as quercetin-3-rhamnoside. High antioxidant and anti-glycation activities of ACRE-E in glycated collagen model indicate its contribution to anti-aging process. A. chinensis rind, previously considered as a byproduct, may have potential as a low-cost raw material for cosmetic and pharmaceutical industries.
KW - Actinidia chinensis
KW - Anti-glycation
KW - Antioxidant
KW - Collagen
KW - Gold kiwi
KW - Quercetrin
UR - http://www.scopus.com/inward/record.url?scp=79960500977&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79960500977&partnerID=8YFLogxK
U2 - 10.3839/jksabc.2011.071
DO - 10.3839/jksabc.2011.071
M3 - Article
AN - SCOPUS:79960500977
VL - 54
SP - 460
EP - 467
JO - Journal of Applied Biological Chemistry
JF - Journal of Applied Biological Chemistry
SN - 1976-0442
IS - 3
ER -