Antioxidant propolis attenuates kainate-induced neurotoxicity via adenosine A1 receptor modulation in the rat

Yong Soo Kwon, Dae Hun Park, Eun Joo Shin, Myung Sang Kwon, Kwang Ho Ko, Won-Ki Kim, Jin Hyeong Jhoo, Wang Kee Jhoo, Myung Bok Wie, Bae Dong Jung, Hyoung Chun Kim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We examined the effects of the antioxidant propolis on seizures induced by kainic acid (KA). Sprague-Dawley rats received propolis (75 and 150 mg/kg, p.o.) five times at 12 h intervals. KA (10 mg/kg, i.p.) was injected 1 h after the last propolis treatment. Pretreatment with propolis significantly attenuated KA-induced seizures and KA-induced increases in hippocampal AP-1 DNA binding activity in a dose-dependent manner. KA induced increases in the levels of malondialdehyde and protein carbonyl, and a decrease in the ratio of GSH/GSSG. These oxidative stresses and neuronal degenerations were significantly attenuated by pretreatment with propolis. The neuroprotective effects of propolis appeared to be counteracted by adenosine receptor antagonists [A 1 antagonist, 8-cyclopentyl-1,3-dimethylxanthine (25 or 50 μg/kg) ; A2A antagonist, 1,3,7-trimethyl-8-(3-chlorostyryl)xanthine (0.5 or 1 mg/kg); and A2B antagonist, alloxazine (1.5 or 3.0 mg/kg)]. However, this counteraction was most pronounced in the presence of the A 1 antagonist. Our results suggest that the protective effect of propolis against KA-induced neurotoxic oxidative damage is, at least in part, via adenosine A1 receptor modulation.

Original languageEnglish
Pages (from-to)231-235
Number of pages5
JournalNeuroscience Letters
Volume355
Issue number3
DOIs
Publication statusPublished - 2004 Jan 30
Externally publishedYes

Fingerprint

Propolis
Adenosine A1 Receptors
Kainic Acid
Antioxidants
Seizures
Purinergic P1 Receptor Antagonists
Glutathione Disulfide
Transcription Factor AP-1
Neuroprotective Agents
Malondialdehyde
Sprague Dawley Rats
Oxidative Stress
DNA

Keywords

  • Adenosine receptors
  • AP-1 DNA binding activity
  • Hippocampus
  • Kainate
  • Oxidative stress
  • Propolis
  • Ratio of GSH/GSSG

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Antioxidant propolis attenuates kainate-induced neurotoxicity via adenosine A1 receptor modulation in the rat. / Kwon, Yong Soo; Park, Dae Hun; Shin, Eun Joo; Kwon, Myung Sang; Ko, Kwang Ho; Kim, Won-Ki; Jhoo, Jin Hyeong; Jhoo, Wang Kee; Wie, Myung Bok; Jung, Bae Dong; Kim, Hyoung Chun.

In: Neuroscience Letters, Vol. 355, No. 3, 30.01.2004, p. 231-235.

Research output: Contribution to journalArticle

Kwon, YS, Park, DH, Shin, EJ, Kwon, MS, Ko, KH, Kim, W-K, Jhoo, JH, Jhoo, WK, Wie, MB, Jung, BD & Kim, HC 2004, 'Antioxidant propolis attenuates kainate-induced neurotoxicity via adenosine A1 receptor modulation in the rat', Neuroscience Letters, vol. 355, no. 3, pp. 231-235. https://doi.org/10.1016/j.neulet.2003.10.075
Kwon, Yong Soo ; Park, Dae Hun ; Shin, Eun Joo ; Kwon, Myung Sang ; Ko, Kwang Ho ; Kim, Won-Ki ; Jhoo, Jin Hyeong ; Jhoo, Wang Kee ; Wie, Myung Bok ; Jung, Bae Dong ; Kim, Hyoung Chun. / Antioxidant propolis attenuates kainate-induced neurotoxicity via adenosine A1 receptor modulation in the rat. In: Neuroscience Letters. 2004 ; Vol. 355, No. 3. pp. 231-235.
@article{8aaf4a8a319a4c7b9f30099b0f99a245,
title = "Antioxidant propolis attenuates kainate-induced neurotoxicity via adenosine A1 receptor modulation in the rat",
abstract = "We examined the effects of the antioxidant propolis on seizures induced by kainic acid (KA). Sprague-Dawley rats received propolis (75 and 150 mg/kg, p.o.) five times at 12 h intervals. KA (10 mg/kg, i.p.) was injected 1 h after the last propolis treatment. Pretreatment with propolis significantly attenuated KA-induced seizures and KA-induced increases in hippocampal AP-1 DNA binding activity in a dose-dependent manner. KA induced increases in the levels of malondialdehyde and protein carbonyl, and a decrease in the ratio of GSH/GSSG. These oxidative stresses and neuronal degenerations were significantly attenuated by pretreatment with propolis. The neuroprotective effects of propolis appeared to be counteracted by adenosine receptor antagonists [A 1 antagonist, 8-cyclopentyl-1,3-dimethylxanthine (25 or 50 μg/kg) ; A2A antagonist, 1,3,7-trimethyl-8-(3-chlorostyryl)xanthine (0.5 or 1 mg/kg); and A2B antagonist, alloxazine (1.5 or 3.0 mg/kg)]. However, this counteraction was most pronounced in the presence of the A 1 antagonist. Our results suggest that the protective effect of propolis against KA-induced neurotoxic oxidative damage is, at least in part, via adenosine A1 receptor modulation.",
keywords = "Adenosine receptors, AP-1 DNA binding activity, Hippocampus, Kainate, Oxidative stress, Propolis, Ratio of GSH/GSSG",
author = "Kwon, {Yong Soo} and Park, {Dae Hun} and Shin, {Eun Joo} and Kwon, {Myung Sang} and Ko, {Kwang Ho} and Won-Ki Kim and Jhoo, {Jin Hyeong} and Jhoo, {Wang Kee} and Wie, {Myung Bok} and Jung, {Bae Dong} and Kim, {Hyoung Chun}",
year = "2004",
month = "1",
day = "30",
doi = "10.1016/j.neulet.2003.10.075",
language = "English",
volume = "355",
pages = "231--235",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Antioxidant propolis attenuates kainate-induced neurotoxicity via adenosine A1 receptor modulation in the rat

AU - Kwon, Yong Soo

AU - Park, Dae Hun

AU - Shin, Eun Joo

AU - Kwon, Myung Sang

AU - Ko, Kwang Ho

AU - Kim, Won-Ki

AU - Jhoo, Jin Hyeong

AU - Jhoo, Wang Kee

AU - Wie, Myung Bok

AU - Jung, Bae Dong

AU - Kim, Hyoung Chun

PY - 2004/1/30

Y1 - 2004/1/30

N2 - We examined the effects of the antioxidant propolis on seizures induced by kainic acid (KA). Sprague-Dawley rats received propolis (75 and 150 mg/kg, p.o.) five times at 12 h intervals. KA (10 mg/kg, i.p.) was injected 1 h after the last propolis treatment. Pretreatment with propolis significantly attenuated KA-induced seizures and KA-induced increases in hippocampal AP-1 DNA binding activity in a dose-dependent manner. KA induced increases in the levels of malondialdehyde and protein carbonyl, and a decrease in the ratio of GSH/GSSG. These oxidative stresses and neuronal degenerations were significantly attenuated by pretreatment with propolis. The neuroprotective effects of propolis appeared to be counteracted by adenosine receptor antagonists [A 1 antagonist, 8-cyclopentyl-1,3-dimethylxanthine (25 or 50 μg/kg) ; A2A antagonist, 1,3,7-trimethyl-8-(3-chlorostyryl)xanthine (0.5 or 1 mg/kg); and A2B antagonist, alloxazine (1.5 or 3.0 mg/kg)]. However, this counteraction was most pronounced in the presence of the A 1 antagonist. Our results suggest that the protective effect of propolis against KA-induced neurotoxic oxidative damage is, at least in part, via adenosine A1 receptor modulation.

AB - We examined the effects of the antioxidant propolis on seizures induced by kainic acid (KA). Sprague-Dawley rats received propolis (75 and 150 mg/kg, p.o.) five times at 12 h intervals. KA (10 mg/kg, i.p.) was injected 1 h after the last propolis treatment. Pretreatment with propolis significantly attenuated KA-induced seizures and KA-induced increases in hippocampal AP-1 DNA binding activity in a dose-dependent manner. KA induced increases in the levels of malondialdehyde and protein carbonyl, and a decrease in the ratio of GSH/GSSG. These oxidative stresses and neuronal degenerations were significantly attenuated by pretreatment with propolis. The neuroprotective effects of propolis appeared to be counteracted by adenosine receptor antagonists [A 1 antagonist, 8-cyclopentyl-1,3-dimethylxanthine (25 or 50 μg/kg) ; A2A antagonist, 1,3,7-trimethyl-8-(3-chlorostyryl)xanthine (0.5 or 1 mg/kg); and A2B antagonist, alloxazine (1.5 or 3.0 mg/kg)]. However, this counteraction was most pronounced in the presence of the A 1 antagonist. Our results suggest that the protective effect of propolis against KA-induced neurotoxic oxidative damage is, at least in part, via adenosine A1 receptor modulation.

KW - Adenosine receptors

KW - AP-1 DNA binding activity

KW - Hippocampus

KW - Kainate

KW - Oxidative stress

KW - Propolis

KW - Ratio of GSH/GSSG

UR - http://www.scopus.com/inward/record.url?scp=12144288041&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12144288041&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2003.10.075

DO - 10.1016/j.neulet.2003.10.075

M3 - Article

C2 - 14732473

AN - SCOPUS:12144288041

VL - 355

SP - 231

EP - 235

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -