Antioxidant propolis attenuates kainate-induced neurotoxicity via adenosine A1 receptor modulation in the rat

Yong Soo Kwon, Dae Hun Park, Eun Joo Shin, Myung Sang Kwon, Kwang Ho Ko, Won-Ki Kim, Jin Hyeong Jhoo, Wang Kee Jhoo, Myung Bok Wie, Bae Dong Jung, Hyoung Chun Kim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We examined the effects of the antioxidant propolis on seizures induced by kainic acid (KA). Sprague-Dawley rats received propolis (75 and 150 mg/kg, p.o.) five times at 12 h intervals. KA (10 mg/kg, i.p.) was injected 1 h after the last propolis treatment. Pretreatment with propolis significantly attenuated KA-induced seizures and KA-induced increases in hippocampal AP-1 DNA binding activity in a dose-dependent manner. KA induced increases in the levels of malondialdehyde and protein carbonyl, and a decrease in the ratio of GSH/GSSG. These oxidative stresses and neuronal degenerations were significantly attenuated by pretreatment with propolis. The neuroprotective effects of propolis appeared to be counteracted by adenosine receptor antagonists [A 1 antagonist, 8-cyclopentyl-1,3-dimethylxanthine (25 or 50 μg/kg) ; A2A antagonist, 1,3,7-trimethyl-8-(3-chlorostyryl)xanthine (0.5 or 1 mg/kg); and A2B antagonist, alloxazine (1.5 or 3.0 mg/kg)]. However, this counteraction was most pronounced in the presence of the A 1 antagonist. Our results suggest that the protective effect of propolis against KA-induced neurotoxic oxidative damage is, at least in part, via adenosine A1 receptor modulation.

Original languageEnglish
Pages (from-to)231-235
Number of pages5
JournalNeuroscience Letters
Volume355
Issue number3
DOIs
Publication statusPublished - 2004 Jan 30
Externally publishedYes

    Fingerprint

Keywords

  • Adenosine receptors
  • AP-1 DNA binding activity
  • Hippocampus
  • Kainate
  • Oxidative stress
  • Propolis
  • Ratio of GSH/GSSG

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kwon, Y. S., Park, D. H., Shin, E. J., Kwon, M. S., Ko, K. H., Kim, W-K., Jhoo, J. H., Jhoo, W. K., Wie, M. B., Jung, B. D., & Kim, H. C. (2004). Antioxidant propolis attenuates kainate-induced neurotoxicity via adenosine A1 receptor modulation in the rat. Neuroscience Letters, 355(3), 231-235. https://doi.org/10.1016/j.neulet.2003.10.075