Antioxidant response of Arabidopsis plants to gamma irradiation: Genome-wide expression profiling of the ROS scavenging and signal transduction pathways

Dong Sub Kim, Jin Baek Kim, Eun Jeong Goh, Wook Jin Kim, Sang Hoon Kim, Yong Weon Seo, Cheol Seong Jang, Si Yong Kang

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Arabidopsis presumably has few sensors for gamma-rays and few signal transduction systems that respond to them. In an effort to assess their radiation sensitivity, wild-type (Ler) Arabidopsis plants were irradiated with various doses of gamma-rays at the vegetative (VE) and reproductive (RE) stages 100 Gy treatment induced the higher production of siliques during both the VE and RE stages compared with non-irradiation. Treatments at doses over 200 Gy repressed shoot growth, and the plants perished under 800 Gy treatment. The results of physiological analysis using electron spin resonance (ESR) and transmission electron microscopy (TEM) revealed that increased doses of gamma-rays induce greater ROS generation. To establish the gene expression profiles after gamma irradiation and for an analysis of the antioxidant response, we employed an oligonucleotide microarray system. Different responses of genes related with ROS scavenging and signal transduction pathways by a gamma irradiation were observed. At least 33 and 42 out of all genes with significantly altered expression were associated with ROS scavenging and signal transduction pathways having an induction or repression ratio cutoff of at least 2-fold, respectively. CAT3 (At1g20620), Ferritin1 (At5g01600), Blue copper binding protein (At5g20230), and AOX putative (At1g32350) were up-regulated regardless of dosage at the VE stage. Reactive oxygen species signaling genes encoding phospholipase, zinc finger protein, WRKY DNA-binding protein, and calcium binding protein were highly expressed, evidencing changes greater than 2-fold. Our transcriptomic profile of the responses of Arabidopsis genes to gamma irradiation showed that plants evidenced altered expressions of many signal transduction and antioxidant genes, as have been seen with other environmental stresses.

Original languageEnglish
Pages (from-to)1960-1971
Number of pages12
JournalJournal of Plant Physiology
Volume168
Issue number16
DOIs
Publication statusPublished - 2011 Nov 1

Fingerprint

Arabidopsis
gamma radiation
signal transduction
Signal Transduction
Antioxidants
Genome
Gamma Rays
antioxidants
genome
Genes
dosage
genes
Plant Shoots
Calcium-Binding Proteins
Phospholipases
Radiation Tolerance
Zinc Fingers
Electron Spin Resonance Spectroscopy
DNA-Binding Proteins
calcium-binding proteins

Keywords

  • Antioxidant response
  • Gamma irradiation
  • ROS scavenging
  • Signal transduction
  • Transcriptome

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

Antioxidant response of Arabidopsis plants to gamma irradiation : Genome-wide expression profiling of the ROS scavenging and signal transduction pathways. / Kim, Dong Sub; Kim, Jin Baek; Goh, Eun Jeong; Kim, Wook Jin; Kim, Sang Hoon; Seo, Yong Weon; Jang, Cheol Seong; Kang, Si Yong.

In: Journal of Plant Physiology, Vol. 168, No. 16, 01.11.2011, p. 1960-1971.

Research output: Contribution to journalArticle

Kim, Dong Sub ; Kim, Jin Baek ; Goh, Eun Jeong ; Kim, Wook Jin ; Kim, Sang Hoon ; Seo, Yong Weon ; Jang, Cheol Seong ; Kang, Si Yong. / Antioxidant response of Arabidopsis plants to gamma irradiation : Genome-wide expression profiling of the ROS scavenging and signal transduction pathways. In: Journal of Plant Physiology. 2011 ; Vol. 168, No. 16. pp. 1960-1971.
@article{9958a74a34704435972050c42d964cbf,
title = "Antioxidant response of Arabidopsis plants to gamma irradiation: Genome-wide expression profiling of the ROS scavenging and signal transduction pathways",
abstract = "Arabidopsis presumably has few sensors for gamma-rays and few signal transduction systems that respond to them. In an effort to assess their radiation sensitivity, wild-type (Ler) Arabidopsis plants were irradiated with various doses of gamma-rays at the vegetative (VE) and reproductive (RE) stages 100 Gy treatment induced the higher production of siliques during both the VE and RE stages compared with non-irradiation. Treatments at doses over 200 Gy repressed shoot growth, and the plants perished under 800 Gy treatment. The results of physiological analysis using electron spin resonance (ESR) and transmission electron microscopy (TEM) revealed that increased doses of gamma-rays induce greater ROS generation. To establish the gene expression profiles after gamma irradiation and for an analysis of the antioxidant response, we employed an oligonucleotide microarray system. Different responses of genes related with ROS scavenging and signal transduction pathways by a gamma irradiation were observed. At least 33 and 42 out of all genes with significantly altered expression were associated with ROS scavenging and signal transduction pathways having an induction or repression ratio cutoff of at least 2-fold, respectively. CAT3 (At1g20620), Ferritin1 (At5g01600), Blue copper binding protein (At5g20230), and AOX putative (At1g32350) were up-regulated regardless of dosage at the VE stage. Reactive oxygen species signaling genes encoding phospholipase, zinc finger protein, WRKY DNA-binding protein, and calcium binding protein were highly expressed, evidencing changes greater than 2-fold. Our transcriptomic profile of the responses of Arabidopsis genes to gamma irradiation showed that plants evidenced altered expressions of many signal transduction and antioxidant genes, as have been seen with other environmental stresses.",
keywords = "Antioxidant response, Gamma irradiation, ROS scavenging, Signal transduction, Transcriptome",
author = "Kim, {Dong Sub} and Kim, {Jin Baek} and Goh, {Eun Jeong} and Kim, {Wook Jin} and Kim, {Sang Hoon} and Seo, {Yong Weon} and Jang, {Cheol Seong} and Kang, {Si Yong}",
year = "2011",
month = "11",
day = "1",
doi = "10.1016/j.jplph.2011.05.008",
language = "English",
volume = "168",
pages = "1960--1971",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "16",

}

TY - JOUR

T1 - Antioxidant response of Arabidopsis plants to gamma irradiation

T2 - Genome-wide expression profiling of the ROS scavenging and signal transduction pathways

AU - Kim, Dong Sub

AU - Kim, Jin Baek

AU - Goh, Eun Jeong

AU - Kim, Wook Jin

AU - Kim, Sang Hoon

AU - Seo, Yong Weon

AU - Jang, Cheol Seong

AU - Kang, Si Yong

PY - 2011/11/1

Y1 - 2011/11/1

N2 - Arabidopsis presumably has few sensors for gamma-rays and few signal transduction systems that respond to them. In an effort to assess their radiation sensitivity, wild-type (Ler) Arabidopsis plants were irradiated with various doses of gamma-rays at the vegetative (VE) and reproductive (RE) stages 100 Gy treatment induced the higher production of siliques during both the VE and RE stages compared with non-irradiation. Treatments at doses over 200 Gy repressed shoot growth, and the plants perished under 800 Gy treatment. The results of physiological analysis using electron spin resonance (ESR) and transmission electron microscopy (TEM) revealed that increased doses of gamma-rays induce greater ROS generation. To establish the gene expression profiles after gamma irradiation and for an analysis of the antioxidant response, we employed an oligonucleotide microarray system. Different responses of genes related with ROS scavenging and signal transduction pathways by a gamma irradiation were observed. At least 33 and 42 out of all genes with significantly altered expression were associated with ROS scavenging and signal transduction pathways having an induction or repression ratio cutoff of at least 2-fold, respectively. CAT3 (At1g20620), Ferritin1 (At5g01600), Blue copper binding protein (At5g20230), and AOX putative (At1g32350) were up-regulated regardless of dosage at the VE stage. Reactive oxygen species signaling genes encoding phospholipase, zinc finger protein, WRKY DNA-binding protein, and calcium binding protein were highly expressed, evidencing changes greater than 2-fold. Our transcriptomic profile of the responses of Arabidopsis genes to gamma irradiation showed that plants evidenced altered expressions of many signal transduction and antioxidant genes, as have been seen with other environmental stresses.

AB - Arabidopsis presumably has few sensors for gamma-rays and few signal transduction systems that respond to them. In an effort to assess their radiation sensitivity, wild-type (Ler) Arabidopsis plants were irradiated with various doses of gamma-rays at the vegetative (VE) and reproductive (RE) stages 100 Gy treatment induced the higher production of siliques during both the VE and RE stages compared with non-irradiation. Treatments at doses over 200 Gy repressed shoot growth, and the plants perished under 800 Gy treatment. The results of physiological analysis using electron spin resonance (ESR) and transmission electron microscopy (TEM) revealed that increased doses of gamma-rays induce greater ROS generation. To establish the gene expression profiles after gamma irradiation and for an analysis of the antioxidant response, we employed an oligonucleotide microarray system. Different responses of genes related with ROS scavenging and signal transduction pathways by a gamma irradiation were observed. At least 33 and 42 out of all genes with significantly altered expression were associated with ROS scavenging and signal transduction pathways having an induction or repression ratio cutoff of at least 2-fold, respectively. CAT3 (At1g20620), Ferritin1 (At5g01600), Blue copper binding protein (At5g20230), and AOX putative (At1g32350) were up-regulated regardless of dosage at the VE stage. Reactive oxygen species signaling genes encoding phospholipase, zinc finger protein, WRKY DNA-binding protein, and calcium binding protein were highly expressed, evidencing changes greater than 2-fold. Our transcriptomic profile of the responses of Arabidopsis genes to gamma irradiation showed that plants evidenced altered expressions of many signal transduction and antioxidant genes, as have been seen with other environmental stresses.

KW - Antioxidant response

KW - Gamma irradiation

KW - ROS scavenging

KW - Signal transduction

KW - Transcriptome

UR - http://www.scopus.com/inward/record.url?scp=80052922547&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052922547&partnerID=8YFLogxK

U2 - 10.1016/j.jplph.2011.05.008

DO - 10.1016/j.jplph.2011.05.008

M3 - Article

C2 - 21665324

AN - SCOPUS:80052922547

VL - 168

SP - 1960

EP - 1971

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

IS - 16

ER -