Antioxidative role of selenoprotein W in oxidant-induced mouse embryonic neuronal cell death

Youn Wook Chung, Daewon Jeong, Ok Jeong Noh, Yong Hwan Park, Soo Im Kang, Min Goo Lee, Tae Hoon Lee, Moon Bin Yim, Ick Young Kim

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Abstract

It has been reported that selenoprotein W (SelW) mRNA is highly expressed in the developing central nerve system of rats, and its expression is maintained until the early postnatal stage. We here found that SelW protein significantly increased in mouse brains of postnatal day 8 and 20 relative to embryonic day 15. This was accompanied by increased expression of SOD1 and SOD2. When the expression of SelW in primary cultured cells derived from embryonic cerebral cortex was knocked down with small interfering RNAs (siRNAs), SelW siRNA-transfected neuronal cells were more sensitive to the oxidative stress induced by treatment of H2O2 than control cells. TUNEL assays revealed that H2O2-induced apoptotic cell death occurred at a higher frequency in the siRNA-transfected cells than in the control cells. Taken together, our findings suggest that SelW plays an important role in protection of neurons from oxidative stress during neuronal development.

Original languageEnglish
Pages (from-to)609-613
Number of pages5
JournalMolecules and Cells
Volume27
Issue number5
DOIs
Publication statusPublished - 2009 Jun 12

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Keywords

  • Antioxidant
  • Neuronal cells
  • Oxidative stress
  • Selenium
  • Seleno protein W

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Chung, Y. W., Jeong, D., Noh, O. J., Park, Y. H., Kang, S. I., Lee, M. G., Lee, T. H., Yim, M. B., & Kim, I. Y. (2009). Antioxidative role of selenoprotein W in oxidant-induced mouse embryonic neuronal cell death. Molecules and Cells, 27(5), 609-613. https://doi.org/10.1007/s10059-009-0074-3