Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic escherichia coli O157: H7

L. E E Kang-Mu, K. I M Wan-Seok, L. I M Jeesun, N. A M Sunyoung, M. I N Youn, N. A M Seong-Won, K. I M Younghoon, Sae Hun Kim, Woojun Park, Sungsu Park

Research output: Contribution to journalArticle

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Abstract

The inhibitory effects of green tea polyphenol epigallocatechin gallate (EGCG) on virulence phenotypes and gene expression regulated by quorum sensing (QS) in Escherichia coli O157:H7 were demonstrated at concentrations of 1 to 100 μg/ml, which are lower than the MIC (539 ± 22 μ-g/ml). At 25 μg/ml, the growth rate was not affected, but autoinducer 2 concentration, biofilm formation, and swarm motility decreased to 13.2, 11.8, and 50%, respectively. Survival at 5 days of nematodes (Caenorhabditis elegans) that were fed the pathogen without and with EGCG were 47.1 and 76%, respectively. Real-time PCR data indicated decreased transcriptional level in many quorum sensing-regulated virulence genes at 25 μg/ml. Our results suggest that EGCG at concentrations below its MIC has significant antipathogenic effects against E. coli O157:H7.

Original languageEnglish
Pages (from-to)325-331
Number of pages7
JournalJournal of Food Protection
Volume72
Issue number2
Publication statusPublished - 2009 Feb 1

Fingerprint

Enterohemorrhagic Escherichia coli
enterohemorrhagic Escherichia coli
Escherichia coli O157
epigallocatechin
Polyphenols
green tea
Tea
Quorum Sensing
polyphenols
quorum sensing
Virulence
virulence
swarms
Caenorhabditis elegans
Biofilms
biofilm
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
Nematoda
Phenotype

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Kang-Mu, L. E. E., Wan-Seok, K. I. M., Jeesun, L. I. M., Sunyoung, N. A. M., Youn, M. I. N., Seong-Won, N. A. M., ... Park, S. (2009). Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic escherichia coli O157: H7. Journal of Food Protection, 72(2), 325-331.

Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic escherichia coli O157 : H7. / Kang-Mu, L. E E; Wan-Seok, K. I M; Jeesun, L. I M; Sunyoung, N. A M; Youn, M. I N; Seong-Won, N. A M; Younghoon, K. I M; Kim, Sae Hun; Park, Woojun; Park, Sungsu.

In: Journal of Food Protection, Vol. 72, No. 2, 01.02.2009, p. 325-331.

Research output: Contribution to journalArticle

Kang-Mu, LEE, Wan-Seok, KIM, Jeesun, LIM, Sunyoung, NAM, Youn, MIN, Seong-Won, NAM, Younghoon, KIM, Kim, SH, Park, W & Park, S 2009, 'Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic escherichia coli O157: H7', Journal of Food Protection, vol. 72, no. 2, pp. 325-331.
Kang-Mu, L. E E ; Wan-Seok, K. I M ; Jeesun, L. I M ; Sunyoung, N. A M ; Youn, M. I N ; Seong-Won, N. A M ; Younghoon, K. I M ; Kim, Sae Hun ; Park, Woojun ; Park, Sungsu. / Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic escherichia coli O157 : H7. In: Journal of Food Protection. 2009 ; Vol. 72, No. 2. pp. 325-331.
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