Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic escherichia coli O157:H7

L. E.E. Kang-Mu, K. I.M. Wan-Seok, L. I.M. Jeesun, N. A.M. Sunyoung, M. I.N. Youn, N. A.M. Seong-Won, K. I.M. Younghoon, K. I.M. Sae-Hun, Woojun Park, Sungsu Park

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Abstract

The inhibitory effects of green tea polyphenol epigallocatechin gallate (EGCG) on virulence phenotypes and gene expression regulated by quorum sensing (QS) in Escherichia coli O157:H7 were demonstrated at concentrations of 1 to 100 μg/ml, which are lower than the MIC (539 ± 22 μ-g/ml). At 25 μg/ml, the growth rate was not affected, but autoinducer 2 concentration, biofilm formation, and swarm motility decreased to 13.2, 11.8, and 50%, respectively. Survival at 5 days of nematodes (Caenorhabditis elegans) that were fed the pathogen without and with EGCG were 47.1 and 76%, respectively. Real-time PCR data indicated decreased transcriptional level in many quorum sensing-regulated virulence genes at 25 μg/ml. Our results suggest that EGCG at concentrations below its MIC has significant antipathogenic effects against E. coli O157:H7.

Original languageEnglish
Pages (from-to)325-331
Number of pages7
JournalJournal of Food Protection
Volume72
Issue number2
Publication statusPublished - 2009 Feb

ASJC Scopus subject areas

  • Food Science
  • Microbiology

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    Kang-Mu, L. E. E., Wan-Seok, K. I. M., Jeesun, L. I. M., Sunyoung, N. A. M., Youn, M. I. N., Seong-Won, N. A. M., Younghoon, K. I. M., Sae-Hun, K. I. M., Park, W., & Park, S. (2009). Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic escherichia coli O157:H7. Journal of Food Protection, 72(2), 325-331.