Antiplatelet effects of rhus verniciflua stokes heartwood and its active constituents -Fisetin, Butein, and Sulfuretin -In Rats

Jun Hyeong Lee, Mikyung Kim, Kyung Hwa Chang, Cheol Yi Hong, Chun Soo Na, Mi Sook Dong, Dongho Lee, Moo Yeol Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Rhus verniciflua stokes (RVS) is known to promote blood circulation by preventing blood stasis, although the active ingredients and the underlying mechanism are unclear. Platelets are the primary cells that regulate circulation and contribute to the development of diverse cardiovascular diseases by aggregation and thrombosis. The study assessed the antiplatelet activity of RVS and sought to identify the active constituents. Pretreatment of washed platelets with RVS heartwood extract blunted the aggregatory response of platelets to collagen. In the subfractions, fisetin, butein, and sulfuretin were identified as effective inhibitors of platelet aggregation by collagen, thrombin, and adenosine-5′-diphosphate. Antiplatelet activities of all three compounds were concentration dependent, and fisetin had longer in vitro duration of action compared with butein or sulfuretin. Extracellular signal-regulated kinase (ERK) mitogen-Activated protein kinase activation by collagen was prevented by fisetin, whereas butein and sulfuretin failed to inhibit ERK and p38 activation was not affected by any of the compounds. Rats orally administered 100mg/(kgday-1) fisetin for 7 days were resistant to arterial thrombosis, although total extract of RVS heartwood exhibited little effect at a dose of 1000mg/(kgday-1). RVS heartwood may have cardiovascular protective activity by inhibiting platelet aggregation. The active constituents are fisetin, butein, and sulfuretin, and fisetin is orally effective against thrombosis.

Original languageEnglish
Pages (from-to)21-30
Number of pages10
JournalJournal of Medicinal Food
Volume18
Issue number1
DOIs
Publication statusPublished - 2015 Jan 1

Keywords

  • Rhus verniciflua stokes
  • antiplatelet
  • butein
  • fisetin
  • sulfuretin

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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