Antitumor effect of a transducible fusogenic peptide releasing multiple proapoptotic peptides by caspase-3

Mi Kyung Kwon, Ju Ock Nam, Rang Woon Park, Byung Heon Lee, Jae Yong Park, Young Ro Byun, Sang Yoon Kim, Ick Chan Kwon, In San Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We have designed a novel peptide, TK3, composed of three functional domains, a protein transduction domain, a TAT followed by three tandem repeats of a proapoptotic peptide, and a caspase-3 cleavage site, (KLAKLAK) 2-DEVD. TK3 was able to transduce into cells and then activate caspase-3, which in turn cleaved TK3 to release additional (KLAKLAK)2 peptides. (KLAKLAK)2 was well transduced by TAT into tumor cells and was able to induce apoptosis in vitro and in vivo. TK3 also induced apoptosis and inhibited angiogenesis in endothelial cells. Further, direct injection of TK3 into established B16F10 melanoma tumors in C57BL/6 mice resulted in almost complete inhibition of the tumor growth. These results suggest that TK3 could be beneficial for the treatment of accessible tumors and used as an adjuvant for cancer therapy.

Original languageEnglish
Pages (from-to)1514-1522
Number of pages9
JournalMolecular cancer therapeutics
Volume7
Issue number6
DOIs
Publication statusPublished - 2008

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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