Antitumor effect of in situ cryoablation with systemic immunotherapy on murine renal cell tumor

Dong Sik Shin, Young Hwii Ko, Hoon Choi, Seok Ho Kang, Jae Hyun Bae, Hong Seok Park, Du Geon Moon, Jun Cheon, Duck Ki Yoon

Research output: Contribution to journalArticle

Abstract

Purpose: To investigate synergistic effect of local cryoablation with systemic immunotherapy, the tumor control ability and immunologic responses of combining these two modalities was compared with that of cryoablation, surgical excision, and immunotherapy only group in a tumor re-challenge model. Materials and Methods: Preliminary experiments were performed in two stages. The first stage consisted of 36 Balb/c mice with Renca bearing tumors imbedded in the right thigh, and was treated with interleukin-2 (IL-2) and interferon-alpha (IFN-α) to evaluate the efficacy of immunotherapy and to determine the adequate dosage. The second stage was performed on 10 mice, to evaluate histological changes and efficacy after cryoablation. The main experiment was performed on 48 mice, divided into 6 groups of control with tumor implantation, excision of tumor, excision combined with immunotherapy, cryoablation of tumor, cryoablation with immunotherapy and control without tumor. After treatment, tumor re-challenge was performed with Renca cell, then the growth pattern was evaluated with physical measurements, and immune response was investigated with fluorescent activated cell sorter and cytotoxicity assay. Results: Preliminary studies on immunologic efficacy revealed that IL-2 and IFN-α have a dose dependent inhibition of tumor growth. The main experiment evaluating the efficacy of combination treatment revealed that cryoablation with immunotherapy proved to be most effective in terms of tumor recurrence and tumor growth inhibition, yet the difference was not statistically significant from monotherapy with cryoablation. However, cytotoxicity was significantly increased cryoablation with immunotherapy compared with other groups. Conclusions: Cryoablation on tumor re-challenge mice model showed advantages with immunotherapy most prominently in cytotoxicity.

Original languageEnglish
Pages (from-to)965-973
Number of pages9
JournalKorean Journal of Urology
Volume49
Issue number11
DOIs
Publication statusPublished - 2008 Nov 1

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Cryosurgery
Immunotherapy
Kidney
Neoplasms
Interferon-alpha
Interleukin-2
Growth
Thigh

Keywords

  • Cryoablation
  • Immunotherapy
  • Renal cell carcinoma

ASJC Scopus subject areas

  • Urology

Cite this

Antitumor effect of in situ cryoablation with systemic immunotherapy on murine renal cell tumor. / Shin, Dong Sik; Ko, Young Hwii; Choi, Hoon; Kang, Seok Ho; Bae, Jae Hyun; Park, Hong Seok; Moon, Du Geon; Cheon, Jun; Yoon, Duck Ki.

In: Korean Journal of Urology, Vol. 49, No. 11, 01.11.2008, p. 965-973.

Research output: Contribution to journalArticle

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abstract = "Purpose: To investigate synergistic effect of local cryoablation with systemic immunotherapy, the tumor control ability and immunologic responses of combining these two modalities was compared with that of cryoablation, surgical excision, and immunotherapy only group in a tumor re-challenge model. Materials and Methods: Preliminary experiments were performed in two stages. The first stage consisted of 36 Balb/c mice with Renca bearing tumors imbedded in the right thigh, and was treated with interleukin-2 (IL-2) and interferon-alpha (IFN-α) to evaluate the efficacy of immunotherapy and to determine the adequate dosage. The second stage was performed on 10 mice, to evaluate histological changes and efficacy after cryoablation. The main experiment was performed on 48 mice, divided into 6 groups of control with tumor implantation, excision of tumor, excision combined with immunotherapy, cryoablation of tumor, cryoablation with immunotherapy and control without tumor. After treatment, tumor re-challenge was performed with Renca cell, then the growth pattern was evaluated with physical measurements, and immune response was investigated with fluorescent activated cell sorter and cytotoxicity assay. Results: Preliminary studies on immunologic efficacy revealed that IL-2 and IFN-α have a dose dependent inhibition of tumor growth. The main experiment evaluating the efficacy of combination treatment revealed that cryoablation with immunotherapy proved to be most effective in terms of tumor recurrence and tumor growth inhibition, yet the difference was not statistically significant from monotherapy with cryoablation. However, cytotoxicity was significantly increased cryoablation with immunotherapy compared with other groups. Conclusions: Cryoablation on tumor re-challenge mice model showed advantages with immunotherapy most prominently in cytotoxicity.",
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