Antitumor effects of herbal mixture extract in the pancreatic adenocarcinoma cell line PANC1

Pyo June Pak, Beob Hwa Kang, Sung Hyo Park, Ji Hyun Sung, Yong Hoon Joo, Seung Hyun Jung, Namhyun Chung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A recent study showned that complementary medicine is gradually gaining wide acceptance. In the present study, the herbal mixture extract (H3) composed of 3 oriental herbal plants was investigated for anticancer activity in vitro and in vivo. H3 inhibited PANC1 cell growth by promoting G0/G1 arrest (11% increase) and apoptotic cell death (9% increase). H3 also suppressed stem cell-like side population cells (4% decrease) and migration activity (24% decrease). In contrast, gemcitabine decreased side population cells and migration activity by 3 and 11%, respectively. These effects of H3 and gemcitabine were further studied by examining the expression of apoptosis-associated genes (CXCR4, JAK2 and XIAP) and stem cell-associated genes (ABCG2, POU5F1 and SOX2). We also found that H3 suppressed tumor growth by 46% in a PANC1-xenograft model, while gemcitabine caused a 36% decrease. The antitumor effects of H3 were confirmed by western blot analysis for COX-2 and cytochrome c expression. Furthermore, necrotic cell death and erythrocyte-containing cavities were detected in tumor tissue by hematoxylin and eosin (HandE) staining. Notably, the combinatorial therapy (H3 and gemcitabine) increased tumor growth compared to that in the control. In conclusion, the present study shows that H3 has promise as a therapeutic agent against pancreatic cancer and its cancer stem cells.

Original languageEnglish
Pages (from-to)2875-2883
Number of pages9
JournalOncology Reports
Volume36
Issue number5
DOIs
Publication statusPublished - 2016 Nov 1

Fingerprint

gemcitabine
Pancreatic Extracts
Adenocarcinoma
Side-Population Cells
Cell Line
Cell Death
Stem Cells
Growth
Neoplasms
Neoplastic Stem Cells
Hematoxylin
Eosine Yellowish-(YS)
Complementary Therapies
Cytochromes c
Pancreatic Neoplasms
Heterografts
Genes
Cell Movement
Erythrocytes
Western Blotting

Keywords

  • Cancer stem cell
  • Pancreatic cancer
  • Side population
  • Traditional medicinal herb
  • Xenograft model

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Pak, P. J., Kang, B. H., Park, S. H., Sung, J. H., Joo, Y. H., Jung, S. H., & Chung, N. (2016). Antitumor effects of herbal mixture extract in the pancreatic adenocarcinoma cell line PANC1. Oncology Reports, 36(5), 2875-2883. https://doi.org/10.3892/or.2016.5067

Antitumor effects of herbal mixture extract in the pancreatic adenocarcinoma cell line PANC1. / Pak, Pyo June; Kang, Beob Hwa; Park, Sung Hyo; Sung, Ji Hyun; Joo, Yong Hoon; Jung, Seung Hyun; Chung, Namhyun.

In: Oncology Reports, Vol. 36, No. 5, 01.11.2016, p. 2875-2883.

Research output: Contribution to journalArticle

Pak, Pyo June ; Kang, Beob Hwa ; Park, Sung Hyo ; Sung, Ji Hyun ; Joo, Yong Hoon ; Jung, Seung Hyun ; Chung, Namhyun. / Antitumor effects of herbal mixture extract in the pancreatic adenocarcinoma cell line PANC1. In: Oncology Reports. 2016 ; Vol. 36, No. 5. pp. 2875-2883.
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