Antiviral Activities of Trichothecenes Isolated from Trichoderma albolutescens against Pepper Mottle Virus

Seung Mok Ryu, Hae Min Lee, Eun Gyeong Song, Young Hye Seo, Jun Lee, Yuanqiang Guo, Beom Seok Kim, Jae-Jin Kim, Jin Sung Hong, Ki Hyun Ryu, Dongho Lee

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A bioassay-guided isolation using a green fluorescence protein (GFP)-tagged pepper mottle virus (PepMoV-GFP) based leaf-disk method to obtain new antiviral agents led to the isolation of trichodermin, 1, and a new compound trichoderminol, 2, from EtOAc extract of Trichoderma albolutescens culture medium. The structures of compounds 1 and 2 were determined by MS and NMR experiments, and the absolute configurations of the compounds were established by experimental and calculated vibrational circular dichroism spectra. Compounds 1 and 2 were evaluated for their anti-PepMoV potential in systemic host plants, such as tobacco and pepper, by PepMoV-GFP based systemic host method. All compounds exhibited inactivation effects against PepMoV. Furthermore, compound 1 showed protective effects against PepMoV.

Original languageEnglish
Pages (from-to)4273-4279
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume65
Issue number21
DOIs
Publication statusPublished - 2017 May 31

Fingerprint

Pepper mottle virus
Trichothecenes
Trichoderma
trichothecenes
Viruses
Antiviral Agents
Fluorescence
Trichodermin
Capsicum
Proteins
Tobacco
Bioassay
fluorescence
Circular Dichroism
Biological Assay
trichodermin
Culture Media
Nuclear magnetic resonance
antiviral agents
circular dichroism spectroscopy

Keywords

  • antiviral agent
  • Capsicum annuum
  • Nicotiana benthamiana
  • pepper mottle virus
  • Trichoderma albolutescens
  • trichothecene
  • vibrational circular dichroism

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Antiviral Activities of Trichothecenes Isolated from Trichoderma albolutescens against Pepper Mottle Virus. / Ryu, Seung Mok; Lee, Hae Min; Song, Eun Gyeong; Seo, Young Hye; Lee, Jun; Guo, Yuanqiang; Kim, Beom Seok; Kim, Jae-Jin; Hong, Jin Sung; Ryu, Ki Hyun; Lee, Dongho.

In: Journal of Agricultural and Food Chemistry, Vol. 65, No. 21, 31.05.2017, p. 4273-4279.

Research output: Contribution to journalArticle

Ryu, Seung Mok ; Lee, Hae Min ; Song, Eun Gyeong ; Seo, Young Hye ; Lee, Jun ; Guo, Yuanqiang ; Kim, Beom Seok ; Kim, Jae-Jin ; Hong, Jin Sung ; Ryu, Ki Hyun ; Lee, Dongho. / Antiviral Activities of Trichothecenes Isolated from Trichoderma albolutescens against Pepper Mottle Virus. In: Journal of Agricultural and Food Chemistry. 2017 ; Vol. 65, No. 21. pp. 4273-4279.
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