Antiviral Activities of Trichothecenes Isolated from Trichoderma albolutescens against Pepper Mottle Virus

Seung Mok Ryu; Hae Min Lee; Eun Gyeong Song; Young Hye Seo; Jun Lee; Yuanqiang Guo; Beom Seok Kim; Jae Jin Kim; Jin Sung Hong; Ki Hyun Ryu; Dongho Lee

doi:10.1021/acs.jafc.7b01028

Antiviral Activities of Trichothecenes Isolated from Trichoderma albolutescens against Pepper Mottle Virus

Seung Mok Ryu, Hae Min Lee, Eun Gyeong Song, Young Hye Seo, Jun Lee, Yuanqiang Guo, Beom Seok Kim, Jae Jin Kim, Jin Sung Hong, Ki Hyun Ryu, Dongho Lee

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

A bioassay-guided isolation using a green fluorescence protein (GFP)-tagged pepper mottle virus (PepMoV-GFP) based leaf-disk method to obtain new antiviral agents led to the isolation of trichodermin, 1, and a new compound trichoderminol, 2, from EtOAc extract of Trichoderma albolutescens culture medium. The structures of compounds 1 and 2 were determined by MS and NMR experiments, and the absolute configurations of the compounds were established by experimental and calculated vibrational circular dichroism spectra. Compounds 1 and 2 were evaluated for their anti-PepMoV potential in systemic host plants, such as tobacco and pepper, by PepMoV-GFP based systemic host method. All compounds exhibited inactivation effects against PepMoV. Furthermore, compound 1 showed protective effects against PepMoV.

Original language	English
Pages (from-to)	4273-4279
Number of pages	7
Journal	Journal of agricultural and food chemistry
Volume	65
Issue number	21
DOIs	https://doi.org/10.1021/acs.jafc.7b01028
Publication status	Published - 2017 May 31

Keywords

Capsicum annuum
Nicotiana benthamiana
Trichoderma albolutescens
antiviral agent
pepper mottle virus
trichothecene
vibrational circular dichroism

ASJC Scopus subject areas

Chemistry(all)
Agricultural and Biological Sciences(all)

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10.1021/acs.jafc.7b01028

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title = "Antiviral Activities of Trichothecenes Isolated from Trichoderma albolutescens against Pepper Mottle Virus",

abstract = "A bioassay-guided isolation using a green fluorescence protein (GFP)-tagged pepper mottle virus (PepMoV-GFP) based leaf-disk method to obtain new antiviral agents led to the isolation of trichodermin, 1, and a new compound trichoderminol, 2, from EtOAc extract of Trichoderma albolutescens culture medium. The structures of compounds 1 and 2 were determined by MS and NMR experiments, and the absolute configurations of the compounds were established by experimental and calculated vibrational circular dichroism spectra. Compounds 1 and 2 were evaluated for their anti-PepMoV potential in systemic host plants, such as tobacco and pepper, by PepMoV-GFP based systemic host method. All compounds exhibited inactivation effects against PepMoV. Furthermore, compound 1 showed protective effects against PepMoV.",

keywords = "Capsicum annuum, Nicotiana benthamiana, Trichoderma albolutescens, antiviral agent, pepper mottle virus, trichothecene, vibrational circular dichroism",

author = "Ryu, {Seung Mok} and Lee, {Hae Min} and Song, {Eun Gyeong} and Seo, {Young Hye} and Jun Lee and Yuanqiang Guo and Kim, {Beom Seok} and Kim, {Jae Jin} and Hong, {Jin Sung} and Ryu, {Ki Hyun} and Dongho Lee",

note = "Funding Information: This research was supported by a grant from the iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries, 115090021SB010) and a grant from National Research Foundation of Korea (NRF- 2015R1D1A1A01060321). Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",

year = "2017",

month = may,

day = "31",

doi = "10.1021/acs.jafc.7b01028",

language = "English",

volume = "65",

pages = "4273--4279",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "21",

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T1 - Antiviral Activities of Trichothecenes Isolated from Trichoderma albolutescens against Pepper Mottle Virus

AU - Ryu, Seung Mok

AU - Lee, Hae Min

AU - Song, Eun Gyeong

AU - Seo, Young Hye

AU - Lee, Jun

AU - Guo, Yuanqiang

AU - Kim, Beom Seok

AU - Kim, Jae Jin

AU - Hong, Jin Sung

AU - Ryu, Ki Hyun

AU - Lee, Dongho

N1 - Funding Information: This research was supported by a grant from the iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries, 115090021SB010) and a grant from National Research Foundation of Korea (NRF- 2015R1D1A1A01060321). Publisher Copyright: © 2017 American Chemical Society.

PY - 2017/5/31

Y1 - 2017/5/31

N2 - A bioassay-guided isolation using a green fluorescence protein (GFP)-tagged pepper mottle virus (PepMoV-GFP) based leaf-disk method to obtain new antiviral agents led to the isolation of trichodermin, 1, and a new compound trichoderminol, 2, from EtOAc extract of Trichoderma albolutescens culture medium. The structures of compounds 1 and 2 were determined by MS and NMR experiments, and the absolute configurations of the compounds were established by experimental and calculated vibrational circular dichroism spectra. Compounds 1 and 2 were evaluated for their anti-PepMoV potential in systemic host plants, such as tobacco and pepper, by PepMoV-GFP based systemic host method. All compounds exhibited inactivation effects against PepMoV. Furthermore, compound 1 showed protective effects against PepMoV.

AB - A bioassay-guided isolation using a green fluorescence protein (GFP)-tagged pepper mottle virus (PepMoV-GFP) based leaf-disk method to obtain new antiviral agents led to the isolation of trichodermin, 1, and a new compound trichoderminol, 2, from EtOAc extract of Trichoderma albolutescens culture medium. The structures of compounds 1 and 2 were determined by MS and NMR experiments, and the absolute configurations of the compounds were established by experimental and calculated vibrational circular dichroism spectra. Compounds 1 and 2 were evaluated for their anti-PepMoV potential in systemic host plants, such as tobacco and pepper, by PepMoV-GFP based systemic host method. All compounds exhibited inactivation effects against PepMoV. Furthermore, compound 1 showed protective effects against PepMoV.

KW - Capsicum annuum

KW - Nicotiana benthamiana

KW - Trichoderma albolutescens

KW - antiviral agent

KW - pepper mottle virus

KW - trichothecene

KW - vibrational circular dichroism

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SN - 0021-8561

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Antiviral Activities of Trichothecenes Isolated from Trichoderma albolutescens against Pepper Mottle Virus

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Keywords

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