Abstract
Human noroviruses are the causative agents of non-bacterial gastroenteritis worldwide. The rapid onset and resolution of disease symptoms suggest that innate immune responses are critical for controlling norovirus infection; however, no effective antivirals are yet available. The present study was conducted to examine the antiviral activities of Schizonepeta tenuifolia Briquet extract (STE) against noroviruses. Treatment of human norovirus replicon-bearing HG23 cells with STE at 5 and 10 mg/ml concentrations resulted in the reduction in the viral RNA levels by 77.2% and 85.9%, respectively. STE had no cytotoxic effects on HG23 cells. Treatment of RAW 264.7 cells infected with murine norovirus 1 (MNV-1), a surrogate virus of human noroviruses, with STE at 10 and 20 µg/ml concentrations resulted in the reduction of viral replication by 58.5% and 84.9%, respectively. STE treatment induced the expression of mRNAs for type I and type II interferons in HG23 cells and upregulated the transcription of interferon-β in infected RAW 264.7 cells via increased phosphorylation of interferon regulatory factor 3, a critical transcription regulator for type I interferon production. These results suggest that STE inhibits norovirus replication through the induction of antiviral interferon production during virus replication and may serve as a candidate antiviral substance for treatment against noroviruses.
| Original language | English |
|---|---|
| Pages (from-to) | 683-689 |
| Number of pages | 7 |
| Journal | Journal of Microbiology |
| Volume | 56 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2018 Sep 1 |
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Keywords
- antiviral activity
- interferons
- natural phytochemicals
- norovirus
- Schizonepeta tenuifolia Briquet (ST)
ASJC Scopus subject areas
- Microbiology
- Applied Microbiology and Biotechnology
Cite this
Antiviral activity of Schizonepeta tenuifolia Briquet against noroviruses via induction of antiviral interferons. / Ng, Yee Ching; Kim, Ye Won; Lee, Jeong Su; Lee, Sung-Joon; Song, Moon Jung.
In: Journal of Microbiology, Vol. 56, No. 9, 01.09.2018, p. 683-689.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antiviral activity of Schizonepeta tenuifolia Briquet against noroviruses via induction of antiviral interferons
AU - Ng, Yee Ching
AU - Kim, Ye Won
AU - Lee, Jeong Su
AU - Lee, Sung-Joon
AU - Song, Moon Jung
PY - 2018/9/1
Y1 - 2018/9/1
N2 - Human noroviruses are the causative agents of non-bacterial gastroenteritis worldwide. The rapid onset and resolution of disease symptoms suggest that innate immune responses are critical for controlling norovirus infection; however, no effective antivirals are yet available. The present study was conducted to examine the antiviral activities of Schizonepeta tenuifolia Briquet extract (STE) against noroviruses. Treatment of human norovirus replicon-bearing HG23 cells with STE at 5 and 10 mg/ml concentrations resulted in the reduction in the viral RNA levels by 77.2% and 85.9%, respectively. STE had no cytotoxic effects on HG23 cells. Treatment of RAW 264.7 cells infected with murine norovirus 1 (MNV-1), a surrogate virus of human noroviruses, with STE at 10 and 20 µg/ml concentrations resulted in the reduction of viral replication by 58.5% and 84.9%, respectively. STE treatment induced the expression of mRNAs for type I and type II interferons in HG23 cells and upregulated the transcription of interferon-β in infected RAW 264.7 cells via increased phosphorylation of interferon regulatory factor 3, a critical transcription regulator for type I interferon production. These results suggest that STE inhibits norovirus replication through the induction of antiviral interferon production during virus replication and may serve as a candidate antiviral substance for treatment against noroviruses.
AB - Human noroviruses are the causative agents of non-bacterial gastroenteritis worldwide. The rapid onset and resolution of disease symptoms suggest that innate immune responses are critical for controlling norovirus infection; however, no effective antivirals are yet available. The present study was conducted to examine the antiviral activities of Schizonepeta tenuifolia Briquet extract (STE) against noroviruses. Treatment of human norovirus replicon-bearing HG23 cells with STE at 5 and 10 mg/ml concentrations resulted in the reduction in the viral RNA levels by 77.2% and 85.9%, respectively. STE had no cytotoxic effects on HG23 cells. Treatment of RAW 264.7 cells infected with murine norovirus 1 (MNV-1), a surrogate virus of human noroviruses, with STE at 10 and 20 µg/ml concentrations resulted in the reduction of viral replication by 58.5% and 84.9%, respectively. STE treatment induced the expression of mRNAs for type I and type II interferons in HG23 cells and upregulated the transcription of interferon-β in infected RAW 264.7 cells via increased phosphorylation of interferon regulatory factor 3, a critical transcription regulator for type I interferon production. These results suggest that STE inhibits norovirus replication through the induction of antiviral interferon production during virus replication and may serve as a candidate antiviral substance for treatment against noroviruses.
KW - antiviral activity
KW - interferons
KW - natural phytochemicals
KW - norovirus
KW - Schizonepeta tenuifolia Briquet (ST)
UR - http://www.scopus.com/inward/record.url?scp=85052237003&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85052237003&partnerID=8YFLogxK
U2 - 10.1007/s12275-018-8228-7
DO - 10.1007/s12275-018-8228-7
M3 - Article
C2 - 30141161
AN - SCOPUS:85052237003
VL - 56
SP - 683
EP - 689
JO - Journal of Microbiology
JF - Journal of Microbiology
SN - 1225-8873
IS - 9
ER -