Apigenin Reduces Survival of Choriocarcinoma Cells by Inducing Apoptosis via the PI3K/AKT and ERK1/2 MAPK Pathways

Whasun Lim, Sunwoo Park, Fuller W. Bazer, Gwonhwa Song

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Apigenin is a flavonoid found in parsley, onions, oranges, tea, chamomile, wheat, and sprouts. It has a variety of biological properties including anti-oxidant, anti-mutagenic, anti-carcinogenic, anti-inflammatory, anti-proliferative, and anti-spasmodic effects. Based on epidemiological and case-control studies, apigenin is regarded as a novel chemotherapeutic agent against various cancer types. However, little is known about the effects of apigenin on choriocarcinoma cells. Therefore, we investigated the anti-cancer effects of apigenin on choriocarcinoma cells (JAR and JEG3) in the present study. Apigenin reduced viability and migratory properties, increased apoptosis, and suppressed mitochondrial membrane potential in both the JAR and JEG3 cells. In addition, apigenin predominantly decreased phosphorylation of AKT, P70RSK, and S6 whereas the phosphorylation of ERK1/2 and P90RSK was increased by apigenin treatment of JAR and JEG3 cells in a dose-dependent manner. Moreover, treatment of JAR and JEG3 cells with both apigenin and pharmacological inhibitors of PI3K/AKT (LY294002) and ERK1/2 (U0126) revealed synergistic anti-proliferative effects. Collectively, these results indicated that the apigenin is an invaluable chemopreventive agent that inhibits progression and metastasis of choriocarcinoma cells through regulation of PI3K/AKT and ERK1/2 MAPK signal transduction mechanism. J. Cell. Physiol. 231: 2690–2699, 2016.

Original languageEnglish
Pages (from-to)2690-2699
Number of pages10
JournalJournal of Cellular Physiology
Volume231
Issue number12
DOIs
Publication statusPublished - 2016 Dec 1

Fingerprint

Apigenin
Choriocarcinoma
MAP Kinase Signaling System
Phosphatidylinositol 3-Kinases
Cell Survival
Apoptosis
Phosphorylation
Chamomile
Petroselinum
S 6
Signal transduction
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Onions
Mitochondrial Membrane Potential
Tea
Flavonoids
Oxidants
Triticum
Case-Control Studies
Signal Transduction

ASJC Scopus subject areas

  • Physiology
  • Medicine(all)
  • Clinical Biochemistry
  • Cell Biology

Cite this

Apigenin Reduces Survival of Choriocarcinoma Cells by Inducing Apoptosis via the PI3K/AKT and ERK1/2 MAPK Pathways. / Lim, Whasun; Park, Sunwoo; Bazer, Fuller W.; Song, Gwonhwa.

In: Journal of Cellular Physiology, Vol. 231, No. 12, 01.12.2016, p. 2690-2699.

Research output: Contribution to journalArticle

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