Apolipoprotein AI and high-density lipoprotein have anti-inflammatory effects on adipocytes via cholesterol transporters

ATP-binding cassette A-1, ATP-binding cassette G-1, and scavenger receptor B-1

Tomio Umemoto, Chang Yeop Han, Poulami Mitra, Michelle M. Averill, Chongren Tang, Leela Goodspeed, Mohamed Omer, Savitha Subramanian, Shari Wang, Laura J. Den Hartigh, Hao Wei, Eung Ju Kim, Jinkyu Kim, Kevin D. O'Brien, Alan Chait

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Rationale: Macrophage accumulation in adipose tissue associates with insulin resistance and increased cardiovascular disease risk. We previously have shown that generation of reactive oxygen species and monocyte chemotactic factors after exposure of adipocytes to saturated fatty acids, such as palmitate, occurs via translocation of NADPH oxidase 4 into lipid rafts (LRs). The anti-inflammatory effects of apolipoprotein AI (apoAI) and high-density lipoprotein (HDL) on macrophages and endothelial cells seem to occur via cholesterol depletion of LRs. However, little is known concerning anti-inflammatory effects of HDL and apoAI on adipocytes. Objective: To determine whether apoAI and HDL inhibit inflammation in adipocytes and adipose tissue, and whether this is dependent on LRs. Methods and Results: In 3T3L-1 adipocytes, apoAI, HDL, and methyl-β-cyclodextrin inhibited chemotactic factor expression. ApoAI and HDL also disrupted LRs, reduced plasma membrane cholesterol content, inhibited NADPH oxidase 4 translocation into LRs, and reduced palmitate-induced reactive oxygen species generation and monocyte chemotactic factor expression. Silencing ATP-binding cassette A-1 abrogated the effect of apoAI, but not HDL, whereas silencing ATP-binding cassette G-1 or scavenger receptor B-1 abrogated the effect of HDL but not apoAI. In vivo, apoAI transgenic mice fed a high-fat, high-sucrose, cholesterol-containing diet showed reduced chemotactic factor and proinflammatory cytokine expression and reduced macrophage accumulation in adipose tissue. Conclusions: ApoAI and HDL have anti-inflammatory effects in adipocytes and adipose tissue similar to their effects in other cell types. These effects are consistent with disruption and removal of cholesterol from LRs, which are regulated by cholesterol transporters, such as ATP-binding cassette A-1, ATP-binding cassette G-1, and scavenger receptor B-1.

Original languageEnglish
Pages (from-to)1345-1354
Number of pages10
JournalCirculation Research
Volume112
Issue number10
DOIs
Publication statusPublished - 2013 Mar 25
Externally publishedYes

Fingerprint

ATP Binding Cassette Transporter 1
Scavenger Receptors
Apolipoproteins
HDL Lipoproteins
Adipocytes
Apolipoprotein A-I
Anti-Inflammatory Agents
Adenosine Triphosphate
Cholesterol
Chemotactic Factors
Lipids
Adipose Tissue
NADPH Oxidase
Palmitates
Macrophages
Monocytes
Reactive Oxygen Species
Cyclodextrins
Transgenic Mice
Sucrose

Keywords

  • ABC transporters
  • Adipocytes
  • Apolipoprotein AI
  • Cholesterol
  • HDL

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Apolipoprotein AI and high-density lipoprotein have anti-inflammatory effects on adipocytes via cholesterol transporters : ATP-binding cassette A-1, ATP-binding cassette G-1, and scavenger receptor B-1. / Umemoto, Tomio; Han, Chang Yeop; Mitra, Poulami; Averill, Michelle M.; Tang, Chongren; Goodspeed, Leela; Omer, Mohamed; Subramanian, Savitha; Wang, Shari; Den Hartigh, Laura J.; Wei, Hao; Kim, Eung Ju; Kim, Jinkyu; O'Brien, Kevin D.; Chait, Alan.

In: Circulation Research, Vol. 112, No. 10, 25.03.2013, p. 1345-1354.

Research output: Contribution to journalArticle

Umemoto, T, Han, CY, Mitra, P, Averill, MM, Tang, C, Goodspeed, L, Omer, M, Subramanian, S, Wang, S, Den Hartigh, LJ, Wei, H, Kim, EJ, Kim, J, O'Brien, KD & Chait, A 2013, 'Apolipoprotein AI and high-density lipoprotein have anti-inflammatory effects on adipocytes via cholesterol transporters: ATP-binding cassette A-1, ATP-binding cassette G-1, and scavenger receptor B-1', Circulation Research, vol. 112, no. 10, pp. 1345-1354. https://doi.org/10.1161/CIRCRESAHA.111.300581
Umemoto, Tomio ; Han, Chang Yeop ; Mitra, Poulami ; Averill, Michelle M. ; Tang, Chongren ; Goodspeed, Leela ; Omer, Mohamed ; Subramanian, Savitha ; Wang, Shari ; Den Hartigh, Laura J. ; Wei, Hao ; Kim, Eung Ju ; Kim, Jinkyu ; O'Brien, Kevin D. ; Chait, Alan. / Apolipoprotein AI and high-density lipoprotein have anti-inflammatory effects on adipocytes via cholesterol transporters : ATP-binding cassette A-1, ATP-binding cassette G-1, and scavenger receptor B-1. In: Circulation Research. 2013 ; Vol. 112, No. 10. pp. 1345-1354.
@article{bc1ef369480c45aa89db5d5ac47a800b,
title = "Apolipoprotein AI and high-density lipoprotein have anti-inflammatory effects on adipocytes via cholesterol transporters: ATP-binding cassette A-1, ATP-binding cassette G-1, and scavenger receptor B-1",
abstract = "Rationale: Macrophage accumulation in adipose tissue associates with insulin resistance and increased cardiovascular disease risk. We previously have shown that generation of reactive oxygen species and monocyte chemotactic factors after exposure of adipocytes to saturated fatty acids, such as palmitate, occurs via translocation of NADPH oxidase 4 into lipid rafts (LRs). The anti-inflammatory effects of apolipoprotein AI (apoAI) and high-density lipoprotein (HDL) on macrophages and endothelial cells seem to occur via cholesterol depletion of LRs. However, little is known concerning anti-inflammatory effects of HDL and apoAI on adipocytes. Objective: To determine whether apoAI and HDL inhibit inflammation in adipocytes and adipose tissue, and whether this is dependent on LRs. Methods and Results: In 3T3L-1 adipocytes, apoAI, HDL, and methyl-β-cyclodextrin inhibited chemotactic factor expression. ApoAI and HDL also disrupted LRs, reduced plasma membrane cholesterol content, inhibited NADPH oxidase 4 translocation into LRs, and reduced palmitate-induced reactive oxygen species generation and monocyte chemotactic factor expression. Silencing ATP-binding cassette A-1 abrogated the effect of apoAI, but not HDL, whereas silencing ATP-binding cassette G-1 or scavenger receptor B-1 abrogated the effect of HDL but not apoAI. In vivo, apoAI transgenic mice fed a high-fat, high-sucrose, cholesterol-containing diet showed reduced chemotactic factor and proinflammatory cytokine expression and reduced macrophage accumulation in adipose tissue. Conclusions: ApoAI and HDL have anti-inflammatory effects in adipocytes and adipose tissue similar to their effects in other cell types. These effects are consistent with disruption and removal of cholesterol from LRs, which are regulated by cholesterol transporters, such as ATP-binding cassette A-1, ATP-binding cassette G-1, and scavenger receptor B-1.",
keywords = "ABC transporters, Adipocytes, Apolipoprotein AI, Cholesterol, HDL",
author = "Tomio Umemoto and Han, {Chang Yeop} and Poulami Mitra and Averill, {Michelle M.} and Chongren Tang and Leela Goodspeed and Mohamed Omer and Savitha Subramanian and Shari Wang and {Den Hartigh}, {Laura J.} and Hao Wei and Kim, {Eung Ju} and Jinkyu Kim and O'Brien, {Kevin D.} and Alan Chait",
year = "2013",
month = "3",
day = "25",
doi = "10.1161/CIRCRESAHA.111.300581",
language = "English",
volume = "112",
pages = "1345--1354",
journal = "Circulation Research",
issn = "0009-7330",
publisher = "Lippincott Williams and Wilkins",
number = "10",

}

TY - JOUR

T1 - Apolipoprotein AI and high-density lipoprotein have anti-inflammatory effects on adipocytes via cholesterol transporters

T2 - ATP-binding cassette A-1, ATP-binding cassette G-1, and scavenger receptor B-1

AU - Umemoto, Tomio

AU - Han, Chang Yeop

AU - Mitra, Poulami

AU - Averill, Michelle M.

AU - Tang, Chongren

AU - Goodspeed, Leela

AU - Omer, Mohamed

AU - Subramanian, Savitha

AU - Wang, Shari

AU - Den Hartigh, Laura J.

AU - Wei, Hao

AU - Kim, Eung Ju

AU - Kim, Jinkyu

AU - O'Brien, Kevin D.

AU - Chait, Alan

PY - 2013/3/25

Y1 - 2013/3/25

N2 - Rationale: Macrophage accumulation in adipose tissue associates with insulin resistance and increased cardiovascular disease risk. We previously have shown that generation of reactive oxygen species and monocyte chemotactic factors after exposure of adipocytes to saturated fatty acids, such as palmitate, occurs via translocation of NADPH oxidase 4 into lipid rafts (LRs). The anti-inflammatory effects of apolipoprotein AI (apoAI) and high-density lipoprotein (HDL) on macrophages and endothelial cells seem to occur via cholesterol depletion of LRs. However, little is known concerning anti-inflammatory effects of HDL and apoAI on adipocytes. Objective: To determine whether apoAI and HDL inhibit inflammation in adipocytes and adipose tissue, and whether this is dependent on LRs. Methods and Results: In 3T3L-1 adipocytes, apoAI, HDL, and methyl-β-cyclodextrin inhibited chemotactic factor expression. ApoAI and HDL also disrupted LRs, reduced plasma membrane cholesterol content, inhibited NADPH oxidase 4 translocation into LRs, and reduced palmitate-induced reactive oxygen species generation and monocyte chemotactic factor expression. Silencing ATP-binding cassette A-1 abrogated the effect of apoAI, but not HDL, whereas silencing ATP-binding cassette G-1 or scavenger receptor B-1 abrogated the effect of HDL but not apoAI. In vivo, apoAI transgenic mice fed a high-fat, high-sucrose, cholesterol-containing diet showed reduced chemotactic factor and proinflammatory cytokine expression and reduced macrophage accumulation in adipose tissue. Conclusions: ApoAI and HDL have anti-inflammatory effects in adipocytes and adipose tissue similar to their effects in other cell types. These effects are consistent with disruption and removal of cholesterol from LRs, which are regulated by cholesterol transporters, such as ATP-binding cassette A-1, ATP-binding cassette G-1, and scavenger receptor B-1.

AB - Rationale: Macrophage accumulation in adipose tissue associates with insulin resistance and increased cardiovascular disease risk. We previously have shown that generation of reactive oxygen species and monocyte chemotactic factors after exposure of adipocytes to saturated fatty acids, such as palmitate, occurs via translocation of NADPH oxidase 4 into lipid rafts (LRs). The anti-inflammatory effects of apolipoprotein AI (apoAI) and high-density lipoprotein (HDL) on macrophages and endothelial cells seem to occur via cholesterol depletion of LRs. However, little is known concerning anti-inflammatory effects of HDL and apoAI on adipocytes. Objective: To determine whether apoAI and HDL inhibit inflammation in adipocytes and adipose tissue, and whether this is dependent on LRs. Methods and Results: In 3T3L-1 adipocytes, apoAI, HDL, and methyl-β-cyclodextrin inhibited chemotactic factor expression. ApoAI and HDL also disrupted LRs, reduced plasma membrane cholesterol content, inhibited NADPH oxidase 4 translocation into LRs, and reduced palmitate-induced reactive oxygen species generation and monocyte chemotactic factor expression. Silencing ATP-binding cassette A-1 abrogated the effect of apoAI, but not HDL, whereas silencing ATP-binding cassette G-1 or scavenger receptor B-1 abrogated the effect of HDL but not apoAI. In vivo, apoAI transgenic mice fed a high-fat, high-sucrose, cholesterol-containing diet showed reduced chemotactic factor and proinflammatory cytokine expression and reduced macrophage accumulation in adipose tissue. Conclusions: ApoAI and HDL have anti-inflammatory effects in adipocytes and adipose tissue similar to their effects in other cell types. These effects are consistent with disruption and removal of cholesterol from LRs, which are regulated by cholesterol transporters, such as ATP-binding cassette A-1, ATP-binding cassette G-1, and scavenger receptor B-1.

KW - ABC transporters

KW - Adipocytes

KW - Apolipoprotein AI

KW - Cholesterol

KW - HDL

UR - http://www.scopus.com/inward/record.url?scp=84879809218&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879809218&partnerID=8YFLogxK

U2 - 10.1161/CIRCRESAHA.111.300581

DO - 10.1161/CIRCRESAHA.111.300581

M3 - Article

VL - 112

SP - 1345

EP - 1354

JO - Circulation Research

JF - Circulation Research

SN - 0009-7330

IS - 10

ER -