Abstract
Apolipoprotein E (APOE) plays a critical role in lipoprotein metabolism by binding to both low-density lipoprotein and APOE receptors. The APOE gene has three allelic forms, ε2, ε3, and ε4, which encode different isoforms of the APOE protein. In this study, we have developed a new genotyping method for APOE. Our multiplex tetra-primer amplification refractory mutation system (multiplex T-ARMS) polymerase chain reaction (PCR) was performed in a single reaction tube with six primers consisting of two common primers and two specific primers for each of two single nucleotide polymorphism (SNP) sites. We obtained definitive electropherograms that showed three (ε2/ε2, ε3/ε3, and ε4/ε4), four (ε2/ε3 and ε3/ε4), and five (ε2/ε4) amplicons by multiplex T-ARMS PCR in a single reaction tube. Multiplex T-ARMS PCR for APOE genotyping is a simple and accurate method that requires only a single PCR reaction, without any another treatments or expensive instrumentation, to simultaneously identify two sites of single nucleotide polymorphisms.
| Original language | English |
|---|---|
| Pages (from-to) | 106-110 |
| Number of pages | 5 |
| Journal | Journal of Biotechnology |
| Volume | 131 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2007 Aug 31 |
Fingerprint
Keywords
- Amplification refractory mutation system
- Apolipoprotein E
- Polymerase chain reaction
- Single nucleotide polymorphism
- Tetra-primer
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Apolipoprotein E genotyping by multiplex tetra-primer amplification refractory mutation system PCR in single reaction tube. / Yang, Young Geun; Kim, Jong Yeol; Park, Su Jeong; Kim, Suhng Wook; Jeon, Ok Hee; Kim, Doo Sik.
In: Journal of Biotechnology, Vol. 131, No. 2, 31.08.2007, p. 106-110.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Apolipoprotein E genotyping by multiplex tetra-primer amplification refractory mutation system PCR in single reaction tube
AU - Yang, Young Geun
AU - Kim, Jong Yeol
AU - Park, Su Jeong
AU - Kim, Suhng Wook
AU - Jeon, Ok Hee
AU - Kim, Doo Sik
PY - 2007/8/31
Y1 - 2007/8/31
N2 - Apolipoprotein E (APOE) plays a critical role in lipoprotein metabolism by binding to both low-density lipoprotein and APOE receptors. The APOE gene has three allelic forms, ε2, ε3, and ε4, which encode different isoforms of the APOE protein. In this study, we have developed a new genotyping method for APOE. Our multiplex tetra-primer amplification refractory mutation system (multiplex T-ARMS) polymerase chain reaction (PCR) was performed in a single reaction tube with six primers consisting of two common primers and two specific primers for each of two single nucleotide polymorphism (SNP) sites. We obtained definitive electropherograms that showed three (ε2/ε2, ε3/ε3, and ε4/ε4), four (ε2/ε3 and ε3/ε4), and five (ε2/ε4) amplicons by multiplex T-ARMS PCR in a single reaction tube. Multiplex T-ARMS PCR for APOE genotyping is a simple and accurate method that requires only a single PCR reaction, without any another treatments or expensive instrumentation, to simultaneously identify two sites of single nucleotide polymorphisms.
AB - Apolipoprotein E (APOE) plays a critical role in lipoprotein metabolism by binding to both low-density lipoprotein and APOE receptors. The APOE gene has three allelic forms, ε2, ε3, and ε4, which encode different isoforms of the APOE protein. In this study, we have developed a new genotyping method for APOE. Our multiplex tetra-primer amplification refractory mutation system (multiplex T-ARMS) polymerase chain reaction (PCR) was performed in a single reaction tube with six primers consisting of two common primers and two specific primers for each of two single nucleotide polymorphism (SNP) sites. We obtained definitive electropherograms that showed three (ε2/ε2, ε3/ε3, and ε4/ε4), four (ε2/ε3 and ε3/ε4), and five (ε2/ε4) amplicons by multiplex T-ARMS PCR in a single reaction tube. Multiplex T-ARMS PCR for APOE genotyping is a simple and accurate method that requires only a single PCR reaction, without any another treatments or expensive instrumentation, to simultaneously identify two sites of single nucleotide polymorphisms.
KW - Amplification refractory mutation system
KW - Apolipoprotein E
KW - Polymerase chain reaction
KW - Single nucleotide polymorphism
KW - Tetra-primer
UR - http://www.scopus.com/inward/record.url?scp=34547543614&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34547543614&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2007.06.001
DO - 10.1016/j.jbiotec.2007.06.001
M3 - Article
C2 - 17643539
AN - SCOPUS:34547543614
VL - 131
SP - 106
EP - 110
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
IS - 2
ER -