Apoptosis in human hepatoma cell lines by chemotherapeutic drugs via Fas-dependent and Fas-independent pathways

Shunai Jiang, Moon Jung Song, Eui Cheol Shin, Mi Ock Lee, Se Jong Kim, Jeon Han Park

Research output: Contribution to journalArticle

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Abstract

Many chemotherapeutic drugs have been found to exert their mode of action via induction of apoptosis in cancer cells. The mechanisms involved in this process are not clear. Recent studies have shown that the Fas/Fas ligand (FasL) system is a key factor controlling apoptotic cell death. In the present study, the involvement of Fas in chemotherapeutic drug-induced apoptosis in hepatoma cell lines was investigated. Five different human hepatoma cell lines, Hep G2, Hep G2.2.15, Hep 3B, SK-Hep-1, and PLC/PRF/5, were used. It was found that they expressed different levels of Fas. However, all five cell lines were susceptible to apoptosis when treated with chemotherapeutic drugs such as 5-fluorouracil (5-FU) or cisplatin. In Hep G2 that constitutively expressed Fas, 5-FU or cisplatin treatment caused an increase in the expression of Fas before the formation of oligonucleosomal DNA fragments, a typical feature of apoptosis. However, in Hep 3B, where Fas is undetectable, apoptosis could also be induced by 5-FU or cisplatin without induction of Fas. The agonistic anti-Fas antibody (CH-11) was capable of inducing apoptosis by itself and promoted drug-induced apoptosis in Hep G2 but not in Hep 3B. The antagonistic anti-Fas antibody (ZB4) inhibited drug- induced apoptosis in Hep G2. Our results suggest that apoptosis can be induced in hepatoma cell lines via both Fas-dependent and Fas-independent pathways.

Original languageEnglish
Pages (from-to)101-110
Number of pages10
JournalHepatology
Volume29
Issue number1
DOIs
Publication statusPublished - 1999 Jan 16
Externally publishedYes

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Hepatocellular Carcinoma
Apoptosis
Cell Line
Pharmaceutical Preparations
Fluorouracil
Cisplatin
Anti-Idiotypic Antibodies
Fas Ligand Protein
Hep G2 Cells
Cell Death
DNA
Neoplasms

ASJC Scopus subject areas

  • Hepatology

Cite this

Apoptosis in human hepatoma cell lines by chemotherapeutic drugs via Fas-dependent and Fas-independent pathways. / Jiang, Shunai; Song, Moon Jung; Shin, Eui Cheol; Lee, Mi Ock; Kim, Se Jong; Park, Jeon Han.

In: Hepatology, Vol. 29, No. 1, 16.01.1999, p. 101-110.

Research output: Contribution to journalArticle

Jiang, Shunai ; Song, Moon Jung ; Shin, Eui Cheol ; Lee, Mi Ock ; Kim, Se Jong ; Park, Jeon Han. / Apoptosis in human hepatoma cell lines by chemotherapeutic drugs via Fas-dependent and Fas-independent pathways. In: Hepatology. 1999 ; Vol. 29, No. 1. pp. 101-110.
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