Apoptosis of neuronal cells in the brains of postembryonic silkworms Bombyx mori (Lepidoptera: Bombycidae)

Mi Young Kim, Kang Min Kim, Chang Ok Choi, Hwa Young Song, Chang Seok Lee, Bong Woo Kim, Pil Don Kang, Soung Hoo Jeon, Byung Pil Cho, Yeon Jae Bae, Young-Gyu Ko, Bong Hee Lee

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Abstract

The pattern and signal transduction of neuronal apoptosis in the brain of the silk moth, Bombyx mori, during postembryonic life, were characterized. Peak numbers of apoptotic neurons were detected in 4 day old 4th instar larvae, 9 day old 5th instar larvae and 4 day old pupae, indicating three waves of neuronal apoptosis during postembryonic development. Most of the apoptotic neurons were in the lateral portions of the brain. No apoptotic neurons were detected in 1 day old 1st instar larvae or in 7 day old pupae to 1 day old adults. Injection of 20-hydroxyecdysone (20E) into larvae resulted in a substantial increase in the brain in both neuronal apoptosis and cleavage of procaspases-8 and -3 into caspases-8 and -3. However, the injection of larvae with actinomycin D or cycloheximide inhibited death of pre-apoptotic neurons. Both the cleavage of procaspases-8 and -3 and death of pre-apoptotic neurons were inhibited by a general caspase inhibitor and caspase-8 and -3 inhibitors injected into larvae. These results suggest that 20E triggered the synthesis of a new protein that, in turn, induces cleavage of procaspases-8 and -3 into caspases-8 and -3. These caspases are prerequisites for neuronal apoptosis in postembryonic brains.

Original languageEnglish
Pages (from-to)335-345
Number of pages11
JournalEuropean Journal of Entomology
Volume106
Issue number3
Publication statusPublished - 2009 Dec 18

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Keywords

  • Actinomycin
  • Brain
  • Caspase
  • Ecdysone
  • Neuronal apoptosis
  • Postembryonic life
  • Silk moth

ASJC Scopus subject areas

  • Insect Science

Cite this

Kim, M. Y., Kim, K. M., Choi, C. O., Song, H. Y., Lee, C. S., Kim, B. W., Kang, P. D., Jeon, S. H., Cho, B. P., Bae, Y. J., Ko, Y-G., & Lee, B. H. (2009). Apoptosis of neuronal cells in the brains of postembryonic silkworms Bombyx mori (Lepidoptera: Bombycidae). European Journal of Entomology, 106(3), 335-345.