Abstract
Introduction: Lengthened immobilization may prevent muscle shortening, and help maintain normal muscle length. However, its apoptotic effects remain unclear. We evaluated the effects of long-term immobilization on apoptotic proteins. Methods: Rat soleus muscles were immobilized by casting in a neutral (NEUT) or lengthened (LENG) position for 21 days. We evaluated dynamic weight load and muscle atrophy following the 21-day period using hematoxylin and eosin staining. We measured Bax (pro-apoptotic Bcl-2 family member), MyoD (myogenic differentiation factor D), MYH (myosin heavy chain), and cleaved poly(ADP-ribose)polymerase levels and examined apoptotic nucleus expression. Results: Decreased dynamic weight load and muscle atrophy changes were observed in LENG. Both NEUT and LENG showed significantly reduced levels of MYH. LENG showed a significant increase in Bax and MyoD expression as well as in the number of apoptotic nuclei. Conclusions: Long-term lengthened immobilization may increase apoptotic changes and decrease muscle formation proteins in muscle. Muscle Nerve, 2018.
| Original language | English |
|---|---|
| Journal | Muscle and Nerve |
| DOIs | |
| Publication status | Accepted/In press - 2018 Jan 1 |
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Keywords
- apoptosis
- Bax
- casting
- lengthened immobilization
- lengthening
- soleus
ASJC Scopus subject areas
- Physiology
- Clinical Neurology
- Cellular and Molecular Neuroscience
- Physiology (medical)
Cite this
Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle. / Suh, Hye Rim; Park, Eui Ho; Moon, Sun Wook; Kim, Ji Won; Cho, Hwi Young; Han, Hee Chul.
In: Muscle and Nerve, 01.01.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle
AU - Suh, Hye Rim
AU - Park, Eui Ho
AU - Moon, Sun Wook
AU - Kim, Ji Won
AU - Cho, Hwi Young
AU - Han, Hee Chul
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Introduction: Lengthened immobilization may prevent muscle shortening, and help maintain normal muscle length. However, its apoptotic effects remain unclear. We evaluated the effects of long-term immobilization on apoptotic proteins. Methods: Rat soleus muscles were immobilized by casting in a neutral (NEUT) or lengthened (LENG) position for 21 days. We evaluated dynamic weight load and muscle atrophy following the 21-day period using hematoxylin and eosin staining. We measured Bax (pro-apoptotic Bcl-2 family member), MyoD (myogenic differentiation factor D), MYH (myosin heavy chain), and cleaved poly(ADP-ribose)polymerase levels and examined apoptotic nucleus expression. Results: Decreased dynamic weight load and muscle atrophy changes were observed in LENG. Both NEUT and LENG showed significantly reduced levels of MYH. LENG showed a significant increase in Bax and MyoD expression as well as in the number of apoptotic nuclei. Conclusions: Long-term lengthened immobilization may increase apoptotic changes and decrease muscle formation proteins in muscle. Muscle Nerve, 2018.
AB - Introduction: Lengthened immobilization may prevent muscle shortening, and help maintain normal muscle length. However, its apoptotic effects remain unclear. We evaluated the effects of long-term immobilization on apoptotic proteins. Methods: Rat soleus muscles were immobilized by casting in a neutral (NEUT) or lengthened (LENG) position for 21 days. We evaluated dynamic weight load and muscle atrophy following the 21-day period using hematoxylin and eosin staining. We measured Bax (pro-apoptotic Bcl-2 family member), MyoD (myogenic differentiation factor D), MYH (myosin heavy chain), and cleaved poly(ADP-ribose)polymerase levels and examined apoptotic nucleus expression. Results: Decreased dynamic weight load and muscle atrophy changes were observed in LENG. Both NEUT and LENG showed significantly reduced levels of MYH. LENG showed a significant increase in Bax and MyoD expression as well as in the number of apoptotic nuclei. Conclusions: Long-term lengthened immobilization may increase apoptotic changes and decrease muscle formation proteins in muscle. Muscle Nerve, 2018.
KW - apoptosis
KW - Bax
KW - casting
KW - lengthened immobilization
KW - lengthening
KW - soleus
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UR - http://www.scopus.com/inward/citedby.url?scp=85058777077&partnerID=8YFLogxK
U2 - 10.1002/mus.26359
DO - 10.1002/mus.26359
M3 - Article
C2 - 30338859
AN - SCOPUS:85058777077
JO - Muscle and Nerve
JF - Muscle and Nerve
SN - 0148-639X
ER -