Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle

Hye Rim Suh; Eui Ho Park; Sun Wook Moon; Ji Won Kim; Hwi Young Cho; Hee Chul Han

doi:10.1002/mus.26359

Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle

Hye Rim Suh, Eui Ho Park, Sun Wook Moon, Ji Won Kim, Hwi Young Cho, Hee Chul Han

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Introduction: Lengthened immobilization may prevent muscle shortening, and help maintain normal muscle length. However, its apoptotic effects remain unclear. We evaluated the effects of long-term immobilization on apoptotic proteins. Methods: Rat soleus muscles were immobilized by casting in a neutral (NEUT) or lengthened (LENG) position for 21 days. We evaluated dynamic weight load and muscle atrophy following the 21-day period using hematoxylin and eosin staining. We measured Bax (pro-apoptotic Bcl-2 family member), MyoD (myogenic differentiation factor D), MYH (myosin heavy chain), and cleaved poly(ADP-ribose)polymerase levels and examined apoptotic nucleus expression. Results: Decreased dynamic weight load and muscle atrophy changes were observed in LENG. Both NEUT and LENG showed significantly reduced levels of MYH. LENG showed a significant increase in Bax and MyoD expression as well as in the number of apoptotic nuclei. Conclusions: Long-term lengthened immobilization may increase apoptotic changes and decrease muscle formation proteins in muscle. Muscle Nerve 59:263–269, 2019.

Original language	English
Pages (from-to)	263-269
Number of pages	7
Journal	Muscle and Nerve
Volume	59
Issue number	2
DOIs	https://doi.org/10.1002/mus.26359
Publication status	Published - 2019 Feb 1

Keywords

Bax
apoptosis
casting
lengthened immobilization
lengthening
soleus

ASJC Scopus subject areas

Physiology
Clinical Neurology
Cellular and Molecular Neuroscience
Physiology (medical)

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10.1002/mus.26359

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title = "Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle",

abstract = "Introduction: Lengthened immobilization may prevent muscle shortening, and help maintain normal muscle length. However, its apoptotic effects remain unclear. We evaluated the effects of long-term immobilization on apoptotic proteins. Methods: Rat soleus muscles were immobilized by casting in a neutral (NEUT) or lengthened (LENG) position for 21 days. We evaluated dynamic weight load and muscle atrophy following the 21-day period using hematoxylin and eosin staining. We measured Bax (pro-apoptotic Bcl-2 family member), MyoD (myogenic differentiation factor D), MYH (myosin heavy chain), and cleaved poly(ADP-ribose)polymerase levels and examined apoptotic nucleus expression. Results: Decreased dynamic weight load and muscle atrophy changes were observed in LENG. Both NEUT and LENG showed significantly reduced levels of MYH. LENG showed a significant increase in Bax and MyoD expression as well as in the number of apoptotic nuclei. Conclusions: Long-term lengthened immobilization may increase apoptotic changes and decrease muscle formation proteins in muscle. Muscle Nerve 59:263–269, 2019.",

keywords = "Bax, apoptosis, casting, lengthened immobilization, lengthening, soleus",

author = "Suh, {Hye Rim} and Park, {Eui Ho} and Moon, {Sun Wook} and Kim, {Ji Won} and Cho, {Hwi Young} and Han, {Hee Chul}",

note = "Funding Information: Abbreviations: Acyp2, acylphosphatase-2; ANOVA, analysis of variance; BAX, pro-apoptotic Bcl-2 family member; CONT, na{\"i}ve rats; DAPI, 4′,6-diamidine-2-phenylidole-dihydrochloride; HDAC6, histone deacety-lase 6; INrf2, Kelch-like ECH-associated protein 1; LENG, lengthened immobilization; MYH, myosin heavy chain; MyoD, myogenic differentiation factor D; NEUT, neutral angle (= 0°) immobilization; PARP, poly (ADP-ribose)polymerase; PBS, phosphate-buffered saline; RT, room temperature; SLC25A4, solute carrier family 25, member 4; TUNEL, terminal deoxynucleotidyl transferase (TdT) –mediated deoxyuridinetriphosphate (dUTP) nick end-labeling Key words: apoptosis, Bax, casting, lengthened immobilization, lengthening, soleus Funding: This research was supported by grants from the Chung Yang, Cha Young Sun & Jang Hi Joo Memorial Fund (Grant 20140101) and the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (Grant 2017R1D1A1B03030568 to H.-R.S.). Publisher Copyright: {\textcopyright} 2018 Wiley Periodicals, Inc.",

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doi = "10.1002/mus.26359",

language = "English",

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journal = "Muscle and Nerve",

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TY - JOUR

T1 - Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle

AU - Suh, Hye Rim

AU - Park, Eui Ho

AU - Moon, Sun Wook

AU - Kim, Ji Won

AU - Cho, Hwi Young

AU - Han, Hee Chul

N1 - Funding Information: Abbreviations: Acyp2, acylphosphatase-2; ANOVA, analysis of variance; BAX, pro-apoptotic Bcl-2 family member; CONT, naïve rats; DAPI, 4′,6-diamidine-2-phenylidole-dihydrochloride; HDAC6, histone deacety-lase 6; INrf2, Kelch-like ECH-associated protein 1; LENG, lengthened immobilization; MYH, myosin heavy chain; MyoD, myogenic differentiation factor D; NEUT, neutral angle (= 0°) immobilization; PARP, poly (ADP-ribose)polymerase; PBS, phosphate-buffered saline; RT, room temperature; SLC25A4, solute carrier family 25, member 4; TUNEL, terminal deoxynucleotidyl transferase (TdT) –mediated deoxyuridinetriphosphate (dUTP) nick end-labeling Key words: apoptosis, Bax, casting, lengthened immobilization, lengthening, soleus Funding: This research was supported by grants from the Chung Yang, Cha Young Sun & Jang Hi Joo Memorial Fund (Grant 20140101) and the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (Grant 2017R1D1A1B03030568 to H.-R.S.). Publisher Copyright: © 2018 Wiley Periodicals, Inc.

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Introduction: Lengthened immobilization may prevent muscle shortening, and help maintain normal muscle length. However, its apoptotic effects remain unclear. We evaluated the effects of long-term immobilization on apoptotic proteins. Methods: Rat soleus muscles were immobilized by casting in a neutral (NEUT) or lengthened (LENG) position for 21 days. We evaluated dynamic weight load and muscle atrophy following the 21-day period using hematoxylin and eosin staining. We measured Bax (pro-apoptotic Bcl-2 family member), MyoD (myogenic differentiation factor D), MYH (myosin heavy chain), and cleaved poly(ADP-ribose)polymerase levels and examined apoptotic nucleus expression. Results: Decreased dynamic weight load and muscle atrophy changes were observed in LENG. Both NEUT and LENG showed significantly reduced levels of MYH. LENG showed a significant increase in Bax and MyoD expression as well as in the number of apoptotic nuclei. Conclusions: Long-term lengthened immobilization may increase apoptotic changes and decrease muscle formation proteins in muscle. Muscle Nerve 59:263–269, 2019.

AB - Introduction: Lengthened immobilization may prevent muscle shortening, and help maintain normal muscle length. However, its apoptotic effects remain unclear. We evaluated the effects of long-term immobilization on apoptotic proteins. Methods: Rat soleus muscles were immobilized by casting in a neutral (NEUT) or lengthened (LENG) position for 21 days. We evaluated dynamic weight load and muscle atrophy following the 21-day period using hematoxylin and eosin staining. We measured Bax (pro-apoptotic Bcl-2 family member), MyoD (myogenic differentiation factor D), MYH (myosin heavy chain), and cleaved poly(ADP-ribose)polymerase levels and examined apoptotic nucleus expression. Results: Decreased dynamic weight load and muscle atrophy changes were observed in LENG. Both NEUT and LENG showed significantly reduced levels of MYH. LENG showed a significant increase in Bax and MyoD expression as well as in the number of apoptotic nuclei. Conclusions: Long-term lengthened immobilization may increase apoptotic changes and decrease muscle formation proteins in muscle. Muscle Nerve 59:263–269, 2019.

KW - Bax

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JO - Muscle and Nerve

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ER -

Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle

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Keywords

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