Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle

Hye Rim Suh, Eui Ho Park, Sun Wook Moon, Ji Won Kim, Hwi Young Cho, Hee Chul Han

Research output: Contribution to journalArticle

Abstract

Introduction: Lengthened immobilization may prevent muscle shortening, and help maintain normal muscle length. However, its apoptotic effects remain unclear. We evaluated the effects of long-term immobilization on apoptotic proteins. Methods: Rat soleus muscles were immobilized by casting in a neutral (NEUT) or lengthened (LENG) position for 21 days. We evaluated dynamic weight load and muscle atrophy following the 21-day period using hematoxylin and eosin staining. We measured Bax (pro-apoptotic Bcl-2 family member), MyoD (myogenic differentiation factor D), MYH (myosin heavy chain), and cleaved poly(ADP-ribose)polymerase levels and examined apoptotic nucleus expression. Results: Decreased dynamic weight load and muscle atrophy changes were observed in LENG. Both NEUT and LENG showed significantly reduced levels of MYH. LENG showed a significant increase in Bax and MyoD expression as well as in the number of apoptotic nuclei. Conclusions: Long-term lengthened immobilization may increase apoptotic changes and decrease muscle formation proteins in muscle. Muscle Nerve, 2018.

Original languageEnglish
JournalMuscle and Nerve
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Fingerprint

Immobilization
Skeletal Muscle
Muscles
Muscular Atrophy
Myosin Heavy Chains
Weights and Measures
Poly(ADP-ribose) Polymerases
Muscle Proteins
Hematoxylin
Eosine Yellowish-(YS)
Staining and Labeling
Proteins

Keywords

  • apoptosis
  • Bax
  • casting
  • lengthened immobilization
  • lengthening
  • soleus

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

Cite this

Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle. / Suh, Hye Rim; Park, Eui Ho; Moon, Sun Wook; Kim, Ji Won; Cho, Hwi Young; Han, Hee Chul.

In: Muscle and Nerve, 01.01.2018.

Research output: Contribution to journalArticle

Suh, Hye Rim ; Park, Eui Ho ; Moon, Sun Wook ; Kim, Ji Won ; Cho, Hwi Young ; Han, Hee Chul. / Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle. In: Muscle and Nerve. 2018.
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