Appetite suppressive effects of yeast hydrolysate on nitric oxide synthase (NOS) expression and vasoactive intestinal peptide (VIP) immunoreactivity in hypothalamus

E. Y. Jung, Hyung Joo Suh, S. Y. Kim, Y. S. Hong, M. J. Kim, U. J. Chang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

To investigate the effects of yeast hydrolysate on appetite regulation mechanisms in the central nervous system, nitric oxide synthase (NOS) expression and vasoactive intestinal peptide (VIP) immunoreactivity in the paraventricular nucleus (PVN) and ventromedial hypothalamic nucleus (VMH) of the hypothalamus were examined. Male Sprague-Dawley (SD) rats were assigned to five groups: control (normal diet), BY-1 and BY-2 (normal diet with oral administration of 0.1 g and 1.0 g of yeast hydrolysate <10 kDa/kg body weight, respectively), AY-1 and AY-2 (normal diet with oral administration of 0.1 g and 1.0 g of yeast hydrolysate 10-30 kDa/kg body weight, respectively). The body weight gain in the BY groups was less than that in the control. In particular, the weight gain of the BY-2 group (133.0 ± 5.1 g) was significantly lower (p < 0.05) than that of the control group (150.1 ± 3.7 g). Among the test groups, the BY-2 group was shown to have significantly lower triacylglycerol (TG) levels (p < 0.05) than the other groups. The staining intensities and optical densities of NOS neurons in the PVN of the AY group were significantly higher (p < 0.05) than in the control and BY groups. The staining intensities and optical densities of VIP immunoreactivity in the PVN and VMH of the BY groups were higher than those of the AY groups and the control. In conclusion, these results indicated that yeast hydrolysate of <10 kDa reduced the body weight gain and body fat in normal diet-fed rats and increased the lipid energy metabolism by altering the expression of NOS and VIP in neurons.

Original languageEnglish
Pages (from-to)1417-1422
Number of pages6
JournalPhytotherapy Research
Volume22
Issue number11
DOIs
Publication statusPublished - 2008 Nov 1

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Vasoactive Intestinal Peptide
Appetite
Nitric Oxide Synthase
Hypothalamus
Paraventricular Hypothalamic Nucleus
Yeasts
Body Weight
Ventromedial Hypothalamic Nucleus
Diet
Weight Gain
Control Groups
Oral Administration
Staining and Labeling
Appetite Regulation
Neurons
Lipid Metabolism
Energy Metabolism
Sprague Dawley Rats
Adipose Tissue
Triglycerides

Keywords

  • Food intake
  • Nitric oxide synthase
  • Vasoactive intestinal peptide
  • Yeast hydrolysate

ASJC Scopus subject areas

  • Pharmacology

Cite this

Appetite suppressive effects of yeast hydrolysate on nitric oxide synthase (NOS) expression and vasoactive intestinal peptide (VIP) immunoreactivity in hypothalamus. / Jung, E. Y.; Suh, Hyung Joo; Kim, S. Y.; Hong, Y. S.; Kim, M. J.; Chang, U. J.

In: Phytotherapy Research, Vol. 22, No. 11, 01.11.2008, p. 1417-1422.

Research output: Contribution to journalArticle

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abstract = "To investigate the effects of yeast hydrolysate on appetite regulation mechanisms in the central nervous system, nitric oxide synthase (NOS) expression and vasoactive intestinal peptide (VIP) immunoreactivity in the paraventricular nucleus (PVN) and ventromedial hypothalamic nucleus (VMH) of the hypothalamus were examined. Male Sprague-Dawley (SD) rats were assigned to five groups: control (normal diet), BY-1 and BY-2 (normal diet with oral administration of 0.1 g and 1.0 g of yeast hydrolysate <10 kDa/kg body weight, respectively), AY-1 and AY-2 (normal diet with oral administration of 0.1 g and 1.0 g of yeast hydrolysate 10-30 kDa/kg body weight, respectively). The body weight gain in the BY groups was less than that in the control. In particular, the weight gain of the BY-2 group (133.0 ± 5.1 g) was significantly lower (p < 0.05) than that of the control group (150.1 ± 3.7 g). Among the test groups, the BY-2 group was shown to have significantly lower triacylglycerol (TG) levels (p < 0.05) than the other groups. The staining intensities and optical densities of NOS neurons in the PVN of the AY group were significantly higher (p < 0.05) than in the control and BY groups. The staining intensities and optical densities of VIP immunoreactivity in the PVN and VMH of the BY groups were higher than those of the AY groups and the control. In conclusion, these results indicated that yeast hydrolysate of <10 kDa reduced the body weight gain and body fat in normal diet-fed rats and increased the lipid energy metabolism by altering the expression of NOS and VIP in neurons.",
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