Application of an enzyme-based biofuel cell containing a bioelectrode modified with deoxyribonucleic acid-wrapped single-walled carbon nanotubes to serum

Jin Young Lee, Hyun Yong Shin, Seong Woo Kang, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Enzyme-based biofuel cells (EFCs) are a form of biofuel cells (BFCs) that can utilize redox enzymes as biocatalysts. Applications of an EFC to an implantable system are evaluated under mild conditions, such as ambient temperature or neutral pH. In the present study, an EFC containing a bioelectrode modified with deoxyribonucleic acid (DNA)-wrapped single-walled carbon nanotubes (SWNTs) was applied to a serum system. The protection of immobilized glucose oxidase (GOD) using DNA-wrapped SWNTs was investigated in a trypsin environment, which can exist in a serum. GOD is immobilized by masking the active site onto the anode electrode. The anode/cathode system in the cell was composed of GOD/laccase as the biocatalysts and glucose/oxygen as the substrates in serum. The electrical properties of the anode in serum according to cyclic voltammetry (CV cycle) were improved using the DNA-wrapped SWNTs. Overall, an EFC that employed DNA-wrapped SWNTs and GOD immobilization in conjunction with protection of the active site increased the stability of GOD in serum, which enabled a high level of power production (ca. 190μW/cm 2) for up to 1 week.

Original languageEnglish
Pages (from-to)80-84
Number of pages5
JournalEnzyme and Microbial Technology
Volume48
Issue number1
DOIs
Publication statusPublished - 2011 Jan 5

Keywords

  • Carbon nanotubes
  • Enzyme activity
  • Enzyme immobilization
  • Enzyme-based biofuel cell
  • Glucose oxidase
  • Serum

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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