Application of an enzyme-linked immunosorbent assay for detecting mold contamination in agricultural commodities and comparison with conventional assays

Je Won Park, Dong Hwa Shon, Young Bae Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Mold contamination in agricultural commodities including grains, grain-based foods, fruits, and vegetables was investigated by two analytical methods. One method employed a mixed monoclonal antibody sandwich ELISA for detection of Aspergillus, Penicillium and Fusarium genera-specific antigens. The detection limit of ELISA for these antigens was 100 ng/g. The other protocol was HPLC-based detection of ergosterol, the predominant sterol in most molds. Recoveries of ergosterol from grains and grain-based foods at spiking levels of 50-1000 ng g-1 ranged between 82 and 86%. The detection limit of the HPLC method for ergosterol was 40 ng g-1. Ninety-two samples (59%) and 116 (75%) among 155 samples tested positive with antigens and ergosterol by the ELISA and the HPLC method, respectively. The cause of conflicting data between both detection methods found in nine dried persimmon samples were solved by using the conventional direct plating method that showed the prevalence of other genera with rarity of Aspergillus, Penicillium and Fusarium. The sandwich ELISA method offers a rapid assay for selectively tracing the potential mycotoxigenic mold, and also is a preliminary screening method for mycotoxin analysis.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalFood and Agricultural Immunology
Volume15
Issue number3-4
DOIs
Publication statusPublished - 2003 Sep 1

Fingerprint

agricultural products
molds (fungi)
Fungi
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
ergosterol
Ergosterol
assays
high performance liquid chromatography
sandwiches
antigens
Penicillium
High Pressure Liquid Chromatography
Aspergillus
Fusarium
methodology
Antigens
detection limit
Limit of Detection
Diospyros

Keywords

  • Agricultural commodities
  • Ergosterol
  • Food
  • Genera-specific mold antigens
  • Mold

ASJC Scopus subject areas

  • Food Science
  • Immunology
  • Agronomy and Crop Science

Cite this

Application of an enzyme-linked immunosorbent assay for detecting mold contamination in agricultural commodities and comparison with conventional assays. / Park, Je Won; Shon, Dong Hwa; Kim, Young Bae.

In: Food and Agricultural Immunology, Vol. 15, No. 3-4, 01.09.2003, p. 159-166.

Research output: Contribution to journalArticle

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