Abstract
Mold contamination in agricultural commodities including grains, grain-based foods, fruits, and vegetables was investigated by two analytical methods. One method employed a mixed monoclonal antibody sandwich ELISA for detection of Aspergillus, Penicillium and Fusarium genera-specific antigens. The detection limit of ELISA for these antigens was 100 ng/g. The other protocol was HPLC-based detection of ergosterol, the predominant sterol in most molds. Recoveries of ergosterol from grains and grain-based foods at spiking levels of 50-1000 ng g-1 ranged between 82 and 86%. The detection limit of the HPLC method for ergosterol was 40 ng g-1. Ninety-two samples (59%) and 116 (75%) among 155 samples tested positive with antigens and ergosterol by the ELISA and the HPLC method, respectively. The cause of conflicting data between both detection methods found in nine dried persimmon samples were solved by using the conventional direct plating method that showed the prevalence of other genera with rarity of Aspergillus, Penicillium and Fusarium. The sandwich ELISA method offers a rapid assay for selectively tracing the potential mycotoxigenic mold, and also is a preliminary screening method for mycotoxin analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 159-166 |
| Number of pages | 8 |
| Journal | Food and Agricultural Immunology |
| Volume | 15 |
| Issue number | 3-4 |
| DOIs | |
| Publication status | Published - 2003 Sep 1 |
Fingerprint
Keywords
- Agricultural commodities
- Ergosterol
- Food
- Genera-specific mold antigens
- Mold
ASJC Scopus subject areas
- Food Science
- Immunology
- Agronomy and Crop Science
Cite this
Application of an enzyme-linked immunosorbent assay for detecting mold contamination in agricultural commodities and comparison with conventional assays. / Park, Je Won; Shon, Dong Hwa; Kim, Young Bae.
In: Food and Agricultural Immunology, Vol. 15, No. 3-4, 01.09.2003, p. 159-166.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Application of an enzyme-linked immunosorbent assay for detecting mold contamination in agricultural commodities and comparison with conventional assays
AU - Park, Je Won
AU - Shon, Dong Hwa
AU - Kim, Young Bae
PY - 2003/9/1
Y1 - 2003/9/1
N2 - Mold contamination in agricultural commodities including grains, grain-based foods, fruits, and vegetables was investigated by two analytical methods. One method employed a mixed monoclonal antibody sandwich ELISA for detection of Aspergillus, Penicillium and Fusarium genera-specific antigens. The detection limit of ELISA for these antigens was 100 ng/g. The other protocol was HPLC-based detection of ergosterol, the predominant sterol in most molds. Recoveries of ergosterol from grains and grain-based foods at spiking levels of 50-1000 ng g-1 ranged between 82 and 86%. The detection limit of the HPLC method for ergosterol was 40 ng g-1. Ninety-two samples (59%) and 116 (75%) among 155 samples tested positive with antigens and ergosterol by the ELISA and the HPLC method, respectively. The cause of conflicting data between both detection methods found in nine dried persimmon samples were solved by using the conventional direct plating method that showed the prevalence of other genera with rarity of Aspergillus, Penicillium and Fusarium. The sandwich ELISA method offers a rapid assay for selectively tracing the potential mycotoxigenic mold, and also is a preliminary screening method for mycotoxin analysis.
AB - Mold contamination in agricultural commodities including grains, grain-based foods, fruits, and vegetables was investigated by two analytical methods. One method employed a mixed monoclonal antibody sandwich ELISA for detection of Aspergillus, Penicillium and Fusarium genera-specific antigens. The detection limit of ELISA for these antigens was 100 ng/g. The other protocol was HPLC-based detection of ergosterol, the predominant sterol in most molds. Recoveries of ergosterol from grains and grain-based foods at spiking levels of 50-1000 ng g-1 ranged between 82 and 86%. The detection limit of the HPLC method for ergosterol was 40 ng g-1. Ninety-two samples (59%) and 116 (75%) among 155 samples tested positive with antigens and ergosterol by the ELISA and the HPLC method, respectively. The cause of conflicting data between both detection methods found in nine dried persimmon samples were solved by using the conventional direct plating method that showed the prevalence of other genera with rarity of Aspergillus, Penicillium and Fusarium. The sandwich ELISA method offers a rapid assay for selectively tracing the potential mycotoxigenic mold, and also is a preliminary screening method for mycotoxin analysis.
KW - Agricultural commodities
KW - Ergosterol
KW - Food
KW - Genera-specific mold antigens
KW - Mold
UR - http://www.scopus.com/inward/record.url?scp=12444303316&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12444303316&partnerID=8YFLogxK
U2 - 10.1080/09540100400013393
DO - 10.1080/09540100400013393
M3 - Article
VL - 15
SP - 159
EP - 166
JO - Food and Agricultural Immunology
JF - Food and Agricultural Immunology
SN - 0954-0105
IS - 3-4
ER -