@article{d5a11a8fbb24426eb40738077eecb522,
title = "Application of M1 macrophage as a live vector in delivering nanoparticles for in vivo photothermal treatment",
abstract = "Introduction: To enhance photothermal treatment (PTT) efficiency, a delivery method that uses cell vector for nanoparticles (NPs) delivery has drawn attention and studied widely in recent years. Objectives: In this study, we demonstrated the feasibility of M1 activated macrophage as a live vector for delivering NPs and investigated the effect of NPs loaded M1 stimulated by Lipopolysaccharide on PTT efficiency in vivo. Methods: M1 was used as a live vector for delivering NPs and further to investigate the effect of NPs loaded M1 on PTT efficiency. Non-activated macrophage (MФ) was stimulated by lipopolysaccharide (LPS) into M1 and assessed for tumor cell phagocytic capacity towards NPs Results: We found M1 exhibited a 20-fold higher uptake capacity of NPs per cell volume and 2.9-fold more active infiltration into the tumor site, compared with non-activated macrophage MФ. We injected M1 cells peritumorally and observed that these cells penetrated into the tumor mass within 12 h. Then, we conducted PTT using irradiation of a near-infrared laser for 1 min at 1 W/cm2. As a result, we confirmed that using M1 as an active live vector led to a more rapid reduction in tumor size within 1 day indicating that the efficacy of PTT with NPs-loaded M1 is higher than that with NPs-loaded MФ. Conclusion: Our study demonstrated the potential role of M1 as a live vector for enhancing the feasibility of PTT in cancer treatment.",
keywords = "Cancer therapy, Live cell vector, M1 macrophage, Medical optics and biotechnology, Photothermal effect, Photothermal treatment",
author = "Im, {Nu Ri} and Yang, {Taeseok Daniel} and Kwanjun Park and Lee, {Jang Hoon} and Jonghwan Lee and {Hyuck Kim}, Yoon and Jae-Seung Lee and Byoungjae Kim and Kwang-Yoon Jung and Youngwoon Choi and Baek, {Seung Kuk}",
note = "Funding Information: We thank Dr. Chang-Min Lee for helpful comments and discussions. This research was supported by the Clinical Trial Center of Korea University Anam Hospital (I1500931), the Korea Health Technology R&D Project (HI14C0748) through the Korea Health Industry Development Institute (KHIDI) by the Ministry of Health & Welfare, and the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2016R1D1A1A02937362, NRF-2018R1D1A1A09083263, NRF-2019R1A2C4004804, and NRF-2019H1A2A1076334), and National Eye Institute (R01EY030569). Institute of Information and Communications Technology Planning and Evaluation (IITP; MSIT) (2020-0-00997). This research was also supported by a grant from Korea University. Funding Information: We thank Dr. Chang-Min Lee for helpful comments and discussions. This research was supported by the Clinical Trial Center of Korea University Anam Hospital (I1500931), the Korea Health Technology R&D Project (HI14C0748) through the Korea Health Industry Development Institute (KHIDI) by the Ministry of Health & Welfare, and the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2016R1D1A1A02937362, NRF-2018R1D1A1A09083263, NRF-2019R1A2C4004804, and NRF-2019H1A2A1076334), and National Eye Institute (R01EY030569). Institute of Information and Communications Technology Planning and Evaluation (IITP; MSIT) (2020-0-00997). This research was also supported by a grant from Korea University. All institutional and national guidelines for the care and use of animals (The Korea University Institutional Animal Care and Use Committee) were followed. Publisher Copyright: {\textcopyright} 2021",
year = "2021",
month = jul,
doi = "10.1016/j.jare.2021.01.010",
language = "English",
volume = "31",
pages = "155--163",
journal = "Journal of Advanced Research",
issn = "2090-1232",
publisher = "Cairo University",
}
