Application of near-infrared fluorescence imaging using a polymeric nanoparticle-based probe for the diagnosis and therapeutic monitoring of colon cancer

Soon Man Yoon, Seung Jae Myung, In Wha Kim, Eun Ju Do, Byong Duk Ye, Ju Hee Ryu, Kyeongsoon Park, Kwang Meyung Kim, Ick Chan Kwon, Mi Jung Kim, Dae Hyuk Moon, Dong Hoon Yang, Kyoung Jo Kim, Jeong Sik Byeon, Suk Kyun Yang, Jin Ho Kim

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Early and accurate detection of adenomatous colonic polyps is a major concern in the prevention of colon cancer. Near-infrared fluorescence (NIRF) imaging with optical probes targeting specific peptides enables the noninvasive visualization and characterization of lesions. Matrix metalloproteinases (MMPs) are known to play an important role in tumorigenesis and tumor progression. Aim: To investigate the effectiveness of NIRF imaging, with a novel MMP-activatable probe based on a polymeric nanoparticle platform, in the colon cancer models. Methods: We used an azoxymethane (AOM)-induced mouse colon cancer model resembling human sporadic colon cancer and an MMP-positive xenograft tumor model. MMP expression was evaluated by Western blotting, real-time PCR, and immunohistochemical staining. NIRF imaging was performed with a novel MMP-activatable probe, an MMP-inactivatable probe, and saline. In addition, we observed the change of NIRF signal intensity after intratumoral administration of an MMP-inhibitor. Results: Multiple tumors with various sizes developed in AOM-treated mouse colons, progressing from adenomas to adenocarcinomas, with MMP expression progressively increasing in the normal-adenoma-adenocarcinoma sequence. In mice injected with the MMP-activatable probe, the NIRF signal also increased in this sequence and was highly correlated with MMP expression (p < 0.001). Tumor-background-ratios (TBR) of adenocarcinoma to adjacent normal mucosa by a novel probe were significantly higher than that of adenoma (p < 0.001). In both the AOM and xenograft models, NIRF signals of tumors decreased after treatment with an MMP-inhibitor. Conclusions: NIRF imaging using a polymeric nanoparticle-based probe may be useful for detecting early stage disease and for assessing treatment response.

Original languageEnglish
Pages (from-to)3005-3013
Number of pages9
JournalDigestive Diseases and Sciences
Volume56
Issue number10
DOIs
Publication statusPublished - 2011 Oct 1
Externally publishedYes

Fingerprint

Optical Imaging
Matrix Metalloproteinases
Nanoparticles
Colonic Neoplasms
Azoxymethane
Adenoma
Adenocarcinoma
Matrix Metalloproteinase Inhibitors
Therapeutics
Fluorescence
Neoplasms
Heterografts
Colonic Polyps
Adenomatous Polyps
Real-Time Polymerase Chain Reaction
Colon
Carcinogenesis
Mucous Membrane
Western Blotting
Staining and Labeling

Keywords

  • Colon cancer
  • Matrix metalloproteinases
  • Nanoparticles
  • Near-infrared fluorescence

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Application of near-infrared fluorescence imaging using a polymeric nanoparticle-based probe for the diagnosis and therapeutic monitoring of colon cancer. / Yoon, Soon Man; Myung, Seung Jae; Kim, In Wha; Do, Eun Ju; Ye, Byong Duk; Ryu, Ju Hee; Park, Kyeongsoon; Kim, Kwang Meyung; Kwon, Ick Chan; Kim, Mi Jung; Moon, Dae Hyuk; Yang, Dong Hoon; Kim, Kyoung Jo; Byeon, Jeong Sik; Yang, Suk Kyun; Kim, Jin Ho.

In: Digestive Diseases and Sciences, Vol. 56, No. 10, 01.10.2011, p. 3005-3013.

Research output: Contribution to journalArticle

Yoon, SM, Myung, SJ, Kim, IW, Do, EJ, Ye, BD, Ryu, JH, Park, K, Kim, KM, Kwon, IC, Kim, MJ, Moon, DH, Yang, DH, Kim, KJ, Byeon, JS, Yang, SK & Kim, JH 2011, 'Application of near-infrared fluorescence imaging using a polymeric nanoparticle-based probe for the diagnosis and therapeutic monitoring of colon cancer', Digestive Diseases and Sciences, vol. 56, no. 10, pp. 3005-3013. https://doi.org/10.1007/s10620-011-1685-z
Yoon, Soon Man ; Myung, Seung Jae ; Kim, In Wha ; Do, Eun Ju ; Ye, Byong Duk ; Ryu, Ju Hee ; Park, Kyeongsoon ; Kim, Kwang Meyung ; Kwon, Ick Chan ; Kim, Mi Jung ; Moon, Dae Hyuk ; Yang, Dong Hoon ; Kim, Kyoung Jo ; Byeon, Jeong Sik ; Yang, Suk Kyun ; Kim, Jin Ho. / Application of near-infrared fluorescence imaging using a polymeric nanoparticle-based probe for the diagnosis and therapeutic monitoring of colon cancer. In: Digestive Diseases and Sciences. 2011 ; Vol. 56, No. 10. pp. 3005-3013.
@article{ea370f542d794f6aa96ae4a461888682,
title = "Application of near-infrared fluorescence imaging using a polymeric nanoparticle-based probe for the diagnosis and therapeutic monitoring of colon cancer",
abstract = "Background: Early and accurate detection of adenomatous colonic polyps is a major concern in the prevention of colon cancer. Near-infrared fluorescence (NIRF) imaging with optical probes targeting specific peptides enables the noninvasive visualization and characterization of lesions. Matrix metalloproteinases (MMPs) are known to play an important role in tumorigenesis and tumor progression. Aim: To investigate the effectiveness of NIRF imaging, with a novel MMP-activatable probe based on a polymeric nanoparticle platform, in the colon cancer models. Methods: We used an azoxymethane (AOM)-induced mouse colon cancer model resembling human sporadic colon cancer and an MMP-positive xenograft tumor model. MMP expression was evaluated by Western blotting, real-time PCR, and immunohistochemical staining. NIRF imaging was performed with a novel MMP-activatable probe, an MMP-inactivatable probe, and saline. In addition, we observed the change of NIRF signal intensity after intratumoral administration of an MMP-inhibitor. Results: Multiple tumors with various sizes developed in AOM-treated mouse colons, progressing from adenomas to adenocarcinomas, with MMP expression progressively increasing in the normal-adenoma-adenocarcinoma sequence. In mice injected with the MMP-activatable probe, the NIRF signal also increased in this sequence and was highly correlated with MMP expression (p < 0.001). Tumor-background-ratios (TBR) of adenocarcinoma to adjacent normal mucosa by a novel probe were significantly higher than that of adenoma (p < 0.001). In both the AOM and xenograft models, NIRF signals of tumors decreased after treatment with an MMP-inhibitor. Conclusions: NIRF imaging using a polymeric nanoparticle-based probe may be useful for detecting early stage disease and for assessing treatment response.",
keywords = "Colon cancer, Matrix metalloproteinases, Nanoparticles, Near-infrared fluorescence",
author = "Yoon, {Soon Man} and Myung, {Seung Jae} and Kim, {In Wha} and Do, {Eun Ju} and Ye, {Byong Duk} and Ryu, {Ju Hee} and Kyeongsoon Park and Kim, {Kwang Meyung} and Kwon, {Ick Chan} and Kim, {Mi Jung} and Moon, {Dae Hyuk} and Yang, {Dong Hoon} and Kim, {Kyoung Jo} and Byeon, {Jeong Sik} and Yang, {Suk Kyun} and Kim, {Jin Ho}",
year = "2011",
month = "10",
day = "1",
doi = "10.1007/s10620-011-1685-z",
language = "English",
volume = "56",
pages = "3005--3013",
journal = "American Journal of Digestive Diseases",
issn = "0002-9211",
publisher = "Springer New York",
number = "10",

}

TY - JOUR

T1 - Application of near-infrared fluorescence imaging using a polymeric nanoparticle-based probe for the diagnosis and therapeutic monitoring of colon cancer

AU - Yoon, Soon Man

AU - Myung, Seung Jae

AU - Kim, In Wha

AU - Do, Eun Ju

AU - Ye, Byong Duk

AU - Ryu, Ju Hee

AU - Park, Kyeongsoon

AU - Kim, Kwang Meyung

AU - Kwon, Ick Chan

AU - Kim, Mi Jung

AU - Moon, Dae Hyuk

AU - Yang, Dong Hoon

AU - Kim, Kyoung Jo

AU - Byeon, Jeong Sik

AU - Yang, Suk Kyun

AU - Kim, Jin Ho

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Background: Early and accurate detection of adenomatous colonic polyps is a major concern in the prevention of colon cancer. Near-infrared fluorescence (NIRF) imaging with optical probes targeting specific peptides enables the noninvasive visualization and characterization of lesions. Matrix metalloproteinases (MMPs) are known to play an important role in tumorigenesis and tumor progression. Aim: To investigate the effectiveness of NIRF imaging, with a novel MMP-activatable probe based on a polymeric nanoparticle platform, in the colon cancer models. Methods: We used an azoxymethane (AOM)-induced mouse colon cancer model resembling human sporadic colon cancer and an MMP-positive xenograft tumor model. MMP expression was evaluated by Western blotting, real-time PCR, and immunohistochemical staining. NIRF imaging was performed with a novel MMP-activatable probe, an MMP-inactivatable probe, and saline. In addition, we observed the change of NIRF signal intensity after intratumoral administration of an MMP-inhibitor. Results: Multiple tumors with various sizes developed in AOM-treated mouse colons, progressing from adenomas to adenocarcinomas, with MMP expression progressively increasing in the normal-adenoma-adenocarcinoma sequence. In mice injected with the MMP-activatable probe, the NIRF signal also increased in this sequence and was highly correlated with MMP expression (p < 0.001). Tumor-background-ratios (TBR) of adenocarcinoma to adjacent normal mucosa by a novel probe were significantly higher than that of adenoma (p < 0.001). In both the AOM and xenograft models, NIRF signals of tumors decreased after treatment with an MMP-inhibitor. Conclusions: NIRF imaging using a polymeric nanoparticle-based probe may be useful for detecting early stage disease and for assessing treatment response.

AB - Background: Early and accurate detection of adenomatous colonic polyps is a major concern in the prevention of colon cancer. Near-infrared fluorescence (NIRF) imaging with optical probes targeting specific peptides enables the noninvasive visualization and characterization of lesions. Matrix metalloproteinases (MMPs) are known to play an important role in tumorigenesis and tumor progression. Aim: To investigate the effectiveness of NIRF imaging, with a novel MMP-activatable probe based on a polymeric nanoparticle platform, in the colon cancer models. Methods: We used an azoxymethane (AOM)-induced mouse colon cancer model resembling human sporadic colon cancer and an MMP-positive xenograft tumor model. MMP expression was evaluated by Western blotting, real-time PCR, and immunohistochemical staining. NIRF imaging was performed with a novel MMP-activatable probe, an MMP-inactivatable probe, and saline. In addition, we observed the change of NIRF signal intensity after intratumoral administration of an MMP-inhibitor. Results: Multiple tumors with various sizes developed in AOM-treated mouse colons, progressing from adenomas to adenocarcinomas, with MMP expression progressively increasing in the normal-adenoma-adenocarcinoma sequence. In mice injected with the MMP-activatable probe, the NIRF signal also increased in this sequence and was highly correlated with MMP expression (p < 0.001). Tumor-background-ratios (TBR) of adenocarcinoma to adjacent normal mucosa by a novel probe were significantly higher than that of adenoma (p < 0.001). In both the AOM and xenograft models, NIRF signals of tumors decreased after treatment with an MMP-inhibitor. Conclusions: NIRF imaging using a polymeric nanoparticle-based probe may be useful for detecting early stage disease and for assessing treatment response.

KW - Colon cancer

KW - Matrix metalloproteinases

KW - Nanoparticles

KW - Near-infrared fluorescence

UR - http://www.scopus.com/inward/record.url?scp=80054766164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054766164&partnerID=8YFLogxK

U2 - 10.1007/s10620-011-1685-z

DO - 10.1007/s10620-011-1685-z

M3 - Article

C2 - 21465144

AN - SCOPUS:80054766164

VL - 56

SP - 3005

EP - 3013

JO - American Journal of Digestive Diseases

JF - American Journal of Digestive Diseases

SN - 0002-9211

IS - 10

ER -