Application of recombinant fluorescent mammalian cells as a toxicity biosensor

E. J. Kim, Y. Lee, J. E. Lee, M. B. Gu

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

With respect to developing a more sensitive biosensor, a recombinant fluorescent Chinese Hamster Ovary cell line was used for the monitoring of various toxicants. Both cell lines, EFC-500 and KFC-A10, were able to detect toxicants sensitively. They were characterized with mitomycin C and γ-ray as genotoxicants and bisphenol A, nonylphenol, ziram and methyl bromide as possible and known EDCs. When compared to each other, the response of KFC-A10 was generally more informative and sensitive. Compared to typical bacterial biosensor systems, these cell lines offered a sensitivity of 2- to 50-fold greater for the tested chemicals. Based on these results, the use of mammalian cells offers a sensitive biosensor system that is not only fast, cheap and reproducible but also capable of monitoring the endocrine-like characteristics of environmental toxicants.

Original languageEnglish
Pages (from-to)51-56
Number of pages6
JournalWater Science and Technology
Volume46
Issue number3
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • GFP
  • Recombinant mammalian cell
  • Toxicity

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

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