Aptamer-based immunosensor on the ZnO nanorods networks

Yoonkyung Nam, Jungil Park, Youngmi Kim Pak, James Jungho Pak

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

This paper presents the fabrication and characteristics of a new aptamer-based electrochemical immunosensor on the patterned zinc oxide nanorod networks (ZNNs) for detecting thrombin. Aptamers are single-stranded RNA or DNA sequence that binds to target materials with high speci?city and af?nity. An antibody-antigen-aptamer sandwich structure was employed to this immunosensor for detecting thrombin. First, hydrothermally grown ZNNs were patterned on the patterned 0.02 cm 2 Au/Ti electrodes on a glass substrate by lift-off process. The high isoelectric point (IEP, ∼9.5) of nanostructured ZnO makes it suitable for immobilizing proteins with low IEP. Then 5 μL of the 500 nM antibody was immobilized on the ZNNs electrode. 5 μL of the mixture of 1 μM aptamer labeled by ferrocene (Fc) and thrombin was dropped on the electrode for antibody-antigen binding. The peak oxidation currents of the immunosensors at various thrombin concentrations were measured by using cyclic voltammetry. The peak oxidation current was observed at 340 mV versus Ag/AgCl electrode, and the peak oxidation current increased linearly from 62.26 nA to 354.13 nA with the logarithmic concentration of thrombin in the range from 100 pM to 250 nM. Fabrication of an aptamer-based immunosensor for thrombin detection is a new attempt and the characteristics of the fabricated immunosensors showed that the fabricated aptamer-baded immunosensor worked electrochemically well and had a low detection limit (∼91.04 pM) and good selectivity.

Original languageEnglish
Pages (from-to)5547-5551
Number of pages5
JournalJournal of Nanoscience and Nanotechnology
Volume12
Issue number7
DOIs
Publication statusPublished - 2012 Jul 1

Fingerprint

thrombin
Immunosensors
Nanotubes
Nanorods
Thrombin
nanorods
Zinc Oxide
Electrodes
Zinc oxide
antibodies
Antibodies
zinc oxides
electrodes
antigens
Antigens
Oxidation
oxidation
Immobilized Antibodies
Fabrication
fabrication

Keywords

  • Aptasensor
  • Electrochemical Immunosensor
  • Thrombin
  • ZnO Nanorods Networks

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Chemistry(all)
  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Aptamer-based immunosensor on the ZnO nanorods networks. / Nam, Yoonkyung; Park, Jungil; Pak, Youngmi Kim; Pak, James Jungho.

In: Journal of Nanoscience and Nanotechnology, Vol. 12, No. 7, 01.07.2012, p. 5547-5551.

Research output: Contribution to journalArticle

Nam, Yoonkyung ; Park, Jungil ; Pak, Youngmi Kim ; Pak, James Jungho. / Aptamer-based immunosensor on the ZnO nanorods networks. In: Journal of Nanoscience and Nanotechnology. 2012 ; Vol. 12, No. 7. pp. 5547-5551.
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