Aptamer biosensor for lable-free detection of human immunoglobulin E based on surface plasmon resonance

Yun Hwa Kim, Jun Pyo Kim, Se Jong Han, Sang Jun Sim

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Aptasensor, an aptamer-based sensor for non-labeled detection of human immunoglobulin E (IgE), was developed using surface plasmon resonance (SPR). Various oligo(ethylene glycol) mixtures of different molar ratios of EG6-COOH and EG3-OH were used for the construction of self-assembled monolayers (SAM). Direct assays and modified sandwich assays over a 1.0 nM anti-IgE, aptamer-immobilized, gold chip, consisting of a 1:3 molar ratio of EG6-COOH:EG3-OH SAM, were investigated for real-time IgE detection. The linear ranges for both were 1.0-1000 ng/mL and the lowest amounts of detectable analytes in the samples were 3.44 and 2.07 ng/mL for direct and modified sandwich assays, respectively. These results for IgE detection suggest that the aptamer offers the possibility of a simple and realistic biosensor platform based on a SAM-fabricated SPR system. Furthermore, as an optic-biosensor, an SPR aptasensor could be used for the diagnosis of allergies.

Original languageEnglish
Pages (from-to)471-475
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume139
Issue number2
DOIs
Publication statusPublished - 2009 Jun 4

Keywords

  • Aptamer
  • Immunoglobulin E (IgE)
  • Self-assembled monolayer (SAM)
  • Surface plasmon resonance (SPR)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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