Aptamer biosensors for label-free colorimetric detection of human IgE based on polydiacetylene (PDA) supramolecules

Jun Pyo Kim, Cheol Hee Park, Sang Jun Sim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In this study, we demonstrate an aptamer-based biosensor (apta-biosensor) using PDA liposomes for label-free detection of allergy diagnosis by hIgE detection. In order to detect the target hIgE, the surface of PDA liposome were functionalized with hIgE antibody and anti-hIgE aptamer as a receptor, and the target hIgE onto the receptors was detected by the change of fluorescence signal. The hIgE antibody-modified PDA liposome biosensor had a serious problem that the immune reaction between receptor and target could not powerfully affect the change of florescence signal on PDA liposome. In order to solve this problem, the anti-hIgE aptamer which was far smaller than whole antibody was introduced as the receptor for the PDA liposome system. An aptamer-based PDA liposome biosensor was able to measure a quantity of target protein with various concentrations and at this time the detection limit was 141 ng/mL of the hIgE concentration. These results enabled diagnosis of allergy disease by an aptamer-based PDA liposome biosensor because real allergic patients showed high concentration of hIgE in serum (greater than 290 ng/mL). Therefore, we suggest that aptamer-modified PDA supramolecules as promising candidates for development of label-free colorimetric biosensors.

Original languageEnglish
Pages (from-to)4269-4274
Number of pages6
JournalJournal of Nanoscience and Nanotechnology
Volume11
Issue number5
DOIs
Publication statusPublished - 2011 May

Keywords

  • Aptamer
  • Biosensor
  • IgE
  • Polydiacetylene

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

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