Aptamers-in-liposomes for selective and multiplexed capture of small organic compounds

Yeon Seok Kim, Javed H. Niazi, Yun Ju Chae, U. Ri Ko, Man Bock Gu

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Small, organic, toxic compounds are not well eliminated by water-treatment systems and eventually become concentrated in the human body. In this study, liposomes are employed to house aptamers with their own binding buffer. When small, organic, toxic compounds in water pass through a liposome barrier, only the target molecules are captured by the DNA aptamers inside the liposomes. The capture efficiency is not high when DNA aptamers are used in tap water. When DNA aptamers in liposomes are used, the capture efficiency increases more than 80%. The simultaneous and selective elimination of target toxicants is successfully performed for tap-water samples containing toxicant mixtures. Aptamer-in-liposome composites are designed and fabricated successfully for three different model aptamers for selective capture of three different target molecules independently or together. This composite of aptamers and liposomes provides a new feature in maintaining the aptamer performance, even in unbuffered samples, and can be applied in selective capture or recovery of target molecules.

Original languageEnglish
Pages (from-to)1169-1173
Number of pages5
JournalMacromolecular Rapid Communications
Volume32
Issue number15
DOIs
Publication statusPublished - 2011 Aug 3

Keywords

  • aptamers
  • liposomes
  • nanocomposites
  • selective capture
  • separation techniques

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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