Aquaporin 3 expression is up-regulated by TGF-β1 in rat peritoneal mesothelial cells and plays a role in wound healing

Hye Myung Ryu, Eun Joo Oh, Sun Hee Park, Chan Duck Kim, Ji Young Choi, Jang Hee Cho, In-San Kim, Tae Hwan Kwon, Ho Young Chung, Min Yoo, Yong Lim Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Aquaporin 3 (AQP3) is expressed in many tissues including the peritoneum and kidney. In cultured mesothelial cells, glucose up-regulates AQP3, which may be important for water transport through the peritoneal membrane. However, there has been no research into the role of AQP3 in human peritoneal mesothelial cell (HPMC) migration or peritoneal fibrosis. We investigated the effects of transforming growth factor-β1 (TGF-β1) on AQP3 expression in HPMCs. We also investigated the role of AQP3 in the peritoneal wound healing process in rats. Chronic exposure to glucose-containing solution increased peritoneal myofibroblasts, with TGF-β1 and AQP3 expression in a model of long-term peritoneal dialysis. In vitro, TGF-β1 induced AQP3 expression in HPMCs. AQP3 knockdown by small-interfering RNA inhibited TGF-β1-induced AQP3 and α-smooth muscle actin expression and also slowed HPMC migration. AQP3 overexpression induced faster migration of HPMCs. Treatment with an extracellular signal-regulated kinase inhibitor and p38 kinase inhibitor attenuated TGF-β1-induced AQP3 expression in HPMCs. These data suggest that TGF-β1 induces AQP3 and that AQP3 has a critical role in TGF-β-induced HPMC migration. These findings provide evidence of a novel role for AQP3 in peritoneal fibrosis and wound healing. The effect of TGF-β1 on AQP3 expression in HPMCs is mediated, at least in part, by ERK and p38 signaling.

Original languageEnglish
Pages (from-to)2047-2057
Number of pages11
JournalAmerican Journal of Pathology
Volume181
Issue number6
DOIs
Publication statusPublished - 2012 Dec 1
Externally publishedYes

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Aquaporin 3
Transforming Growth Factors
Wound Healing
Peritoneal Fibrosis
Cell Movement
Glucose

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Aquaporin 3 expression is up-regulated by TGF-β1 in rat peritoneal mesothelial cells and plays a role in wound healing. / Ryu, Hye Myung; Oh, Eun Joo; Park, Sun Hee; Kim, Chan Duck; Choi, Ji Young; Cho, Jang Hee; Kim, In-San; Kwon, Tae Hwan; Chung, Ho Young; Yoo, Min; Kim, Yong Lim.

In: American Journal of Pathology, Vol. 181, No. 6, 01.12.2012, p. 2047-2057.

Research output: Contribution to journalArticle

Ryu, HM, Oh, EJ, Park, SH, Kim, CD, Choi, JY, Cho, JH, Kim, I-S, Kwon, TH, Chung, HY, Yoo, M & Kim, YL 2012, 'Aquaporin 3 expression is up-regulated by TGF-β1 in rat peritoneal mesothelial cells and plays a role in wound healing', American Journal of Pathology, vol. 181, no. 6, pp. 2047-2057. https://doi.org/10.1016/j.ajpath.2012.08.018
Ryu, Hye Myung ; Oh, Eun Joo ; Park, Sun Hee ; Kim, Chan Duck ; Choi, Ji Young ; Cho, Jang Hee ; Kim, In-San ; Kwon, Tae Hwan ; Chung, Ho Young ; Yoo, Min ; Kim, Yong Lim. / Aquaporin 3 expression is up-regulated by TGF-β1 in rat peritoneal mesothelial cells and plays a role in wound healing. In: American Journal of Pathology. 2012 ; Vol. 181, No. 6. pp. 2047-2057.
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AU - Kim, Chan Duck

AU - Choi, Ji Young

AU - Cho, Jang Hee

AU - Kim, In-San

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AU - Yoo, Min

AU - Kim, Yong Lim

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