Aqueous ammonia pretreatment, saccharification, and fermentation evaluation of oil palm fronds for ethanol production

Young Hoon Jung, Sooah Kim, Taek Ho Yang, Hee Jong Lee, Doyoung Seung, Yong Cheol Park, Jin Ho Seo, In Geol Choi, Kyoung Heon Kim

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Oil palm fronds are the most abundant lignocellulosic biomass in Malaysia. In this study, fronds were tested as the potential renewable biomass for ethanol production. The soaking in aqueous ammonia pretreatment was applied, and the fermentability of pretreated fronds was evaluated using simultaneous saccharification and fermentation. The optimal pretreatment conditions were 7 % (w/w) ammonia, 80 °C, 20 h of pretreatment, and 1:12 S/L ratio, where the enzymatic digestibility was 41.4 % with cellulase of 60 FPU/g-glucan. When increasing the cellulase loading in the hydrolysis of pretreated fronds, the enzymatic digestibility increased until the enzyme loading reached 60 FPU/g-glucan. With 3 % glucan loading in the SSF of pretreated fronds, the ethanol concentration and yield based on the theoretical maximum after 12 and 48 h of the SSF were 7.5 and 9.7 g/L and 43.8 and 56.8 %, respectively. The ethanol productivities found at 12 and 24 h from pretreated fronds were 0.62 and 0.36 g/L/h, respectively.

Original languageEnglish
Pages (from-to)1497-1503
Number of pages7
JournalBioprocess and Biosystems Engineering
Volume35
Issue number9
DOIs
Publication statusPublished - 2012 Nov 1

Keywords

  • Ammonia
  • Ethanol
  • Lignocellulose
  • Oil palm fronds
  • Pretreatment

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

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